Lorraine Mosqueda
San Jose,California
Summary
Dedicated and accomplished Senior Associate Scientist with 9 years of experience in the Biotechnology industry. Proven expertise in designing and executing scientific experiments, analyzing complex data, and driving research projects to successful outcomes. Skilled in leading cross-functional teams, mentoring junior researchers, and collaborating with stakeholders to advance scientific initiatives.
Overview
9
9
years of professional experience
Work History
Associate Scientist
Hengenix
09.2022 - Current
- Routinely executed surface and intracellular staining for PBMCs and other cell lines (Jurkat, CHOK1, HEPG2, A549, A498, THP-1, 22RV1, MDA-MB-231, JEG-3,CT26, SW480, etc) to read on FACs machines
- Proficiently executed cell culture procedures, ensuring optimal maintenance of cell lines and adhered to storage practices to ensure cell viability and integrity
- Performed binding, proliferation, and ADCC Reporter assays to study MOA of antibody candidates
- Executed TDCC tumor killing assays using human PBMCs and Isolated T cells
- Detected IL-2 induced CD8 and NK cells in pSTAT5, proliferation, and activation
- Analyzed levels of TNFa, IFNG, GM-CSF, Granzyme B, IL6, IL10 and other cytokines on monocyte-derived macrophages using AlphaLISA and LegendPlex
- Routinely isolated PBMCs from buffy coats and Leukopac to maintain working bank of frozen cells to set-up experiments that require fresh PBMCs
- Regularly established detailed flow panels that included between 6-13 fluorophores and analyzed data using CellEngine software
- Familiar with different FACs Machine: Cytoflex, Cytek, and Attune
Senior Associate Scientist
Strateos
06.2019 - 09.2022
- One of the main contributors to the company's $1.5M total production revenue in 2021, executing over 200+ runs
- Onboarded and troubleshoot manual scientific processes onto automated systems
- Technical lead for automation validation post software deployment
- Worked with cross functional teams to develop more effective UX for automation platform
- Performed molecular, nucleic, and synthetic biology assays utilizing robotic instrumentations (DNA, RNA, Protein Purification using KingfisherFlex, single/multiplex qPCR using Bio-Rad thermocyclers, determined cell viability by establishing an automated cell-titer glo assay, and automating a TCID50 assay within mammalian cells to determine viral titers of viruses)
- Revised and generated new SOPs to capture accurate troubleshooting steps and trained coworkers on automated platforms
Senior Laboratory Technician
Genomic Health
01.2015 - 06.2019
- Identified, escalated, and lead an implemented process improvement for managing 800+ patient samples per day
- Managed sample pooling and disposal projects that improved efficiency of the process by reducing production time by an hour
- Calibrated, validated and maintained laboratory equipment
- Operated and performed preventative maintenance on laboratory equipment and instruments
- Accurately documented and maintained all records for equipment and instruments, contributing to ISO/CAPA audits
- Kept equipment maintained, facility clean and organized and inventory stocked to maximize performance
- Kept laboratory in compliance with applicable guidelines and laws
- Trained and supervised junior technicians in laboratory protocols
- Designed processes to increase efficiency by minimizing error and reducing repetitive tasks
QA/QC Inspector
Abbvie StemCentrx LLC, Synectics Inc
02.2018 - 03.2019
- Performed ELISA and cell-based methods to meet lot release and stability testing
- Performed qualification of bioassay methods in support of cGMP activities
- Supported various aspects of QC testing for antibody and antibody drug conjugates
- Administered tests to assess whether engineers and operators were qualified to use equipment
- Discarded and rejected products, materials and equipment not meeting specifications
- Operated, calibrated and maintained testing equipment
- Inspected products, components and materials for defects and compliance with tolerance standards
- Utilized visual inspection techniques as well as performing detailed measurements with hand-held tools and devices
Education
Bachelor of Science - Microbial Biology, Global Poverty & Practice
University of California, Berkeley
Berkeley, CA05.2014
Skills
- Lab Equipment Calibration
- Salesforce Software
- Personnel Training
- Scientific Methodologies
- Daily Logs
- Supply Ordering
- Customer Satisfaction
- Chemical Testing
- Research
- Stability Sampling
- Presentations and White Papers
- Safety Standards
- Disposal Procedures
- Clean Equipment
- Research and Publication
- Document Organization
- Instructional Strategies
- Microsoft Excel
- Electronic Lab Notebook Documentation
- Clinical Studies
- Critical Thinking
- Standard Operating Procedure Development
- Minor Repairs
- Lab Experiments and Research
- Data Analytics
- Documenting Changes
- Microsoft Project
- Social Perceptiveness
- Active Learning
- Lab Scheduling
- Excellent Written and Verbal Communication
- Test Data Summarization
- Microsoft SharePoint
- Reading Comprehension
- Packaging Equipment
- Basic Computer Skills
- Public Speaking
- Complex Problem-Solving
- Validation Protocols
- Electrical Devices
- Protocol Review
- GxP Compliance
- Lab Safety
- Assay Qualification Methods
- Administrative Support
- Safety Processes
- ELISA Development
- Data Integrity
- Laboratory Equipment Functions
- Computerized Maintenance Management Systems
- Chemical and Molecular Analysis
- Quality Production
- Corrective Actions
- FDA Regulations
- Study Protocols
- Scientific Principles Application
- Project Requirements
- Upstream and Downstream Processing
- Process Automation and Optimization
- CGMP Regulations
- Patient Information
- Results Analysis
- Vendor Contracts
- New Technology Innovation
- Data Accuracy
- Method Validation
- Assay Development
- Experiment Protocol Development
Publications
- Carlson HK, Stoeva MK, Justice NB, Sczesnak A, Mullan MR, Mosqueda LA, Kuehl JV, Deutschbauer AM, Arkin AP, Coates JD. (2015) Monofluorophosphate is a selective inhibitor of respiratory sulfate-reducing microorganisms. Environmental Science & Technology.
- Goldman RP, Moseley R, Roehner N, Cummins B, Vrana JD, Clowers KJ, Bryce D, Beal J, DeHaven M, Nowak J, Higa T, Biggers V, Lee P, Hunt JP, Mosqueda L, Haase SB, Weston M, Zheng G, Deckard A, Gopaulakrishnan S, Stubbs JF, Gaffney NI, Vaughn MW, Maheshri N, Mikhalev E, Bartley B, Markeloff R, Mitchell T, Nguyen T, Sumorok D, Walczak N, Myers C, Zundel Z, Hatch B, Scholz J, Colonna-Romano J. (2022) Highly-automated, high-throughput replication of yeast-based logic circuit design assessments. Synthetic Biology (Oxford).
- Cummins B, Vrana J, Moseley RC, Eramian H, Deckard A, Fontanarrosa P, Bryce D, Weston M, Zheng G, Nowak J, Motta FC, Eslami M, Johnson KL, Goldman RP, Myers CJ, Johnson T, Vaughn MW, Gaffney N, Urrutia J, Gopaulakrishnan S, Biggers V, Higa TR, Mosqueda LA, Gameiro M, Gedeon T, Mischaikow K, Beal J, Bartley B, Mitchell T, Nguyen TT, Roehner N, Haase SB. (2023) Robustness and reproducibility of simple and complex synthetic logic circuit designs using a DBTL loop. Synthetic Biology (Oxford).
Work Availability
monday
tuesday
wednesday
thursday
friday
saturday
sunday
morning
afternoon
evening
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Quote
The best and safest thing is to keep a balance in your life, acknowledge the great powers around us and in us. If you can do that, and live that way, you are really a wise man.
Euripides
Timeline
Associate Scientist
Hengenix
09.2022 - Current
Senior Associate Scientist
Strateos
06.2019 - 09.2022
QA/QC Inspector
Abbvie StemCentrx LLC, Synectics Inc
02.2018 - 03.2019
Senior Laboratory Technician
Genomic Health
01.2015 - 06.2019
Bachelor of Science - Microbial Biology, Global Poverty & Practice
University of California, Berkeley
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