Lucia Chea
San Diego,CA
Summary
Biochemist with 3+ years of biotechnology industry experience in Bioconjugation, validation, and quality control. Extensive skills in development of immunoassays and oligonucleotide conjugation, , and molecular biology. Proficient in establishing flow-cytometry and other cell-based assays to evaluate bioactivity/potency of biologics.
Overview
4
4
years of professional experience
Work History
Biochemist II
Revvity (BioLegend)
San Diego, CA
05.2021 - Current
- Developed high-sensitivity immunoassays, such as ELISAs for commercial manufacture in to kits.
- Purified monoclonal and polyclonal antibodies using affinity, ion-exchange, and size-exclusion chromatography on GE-Akta FPLC system.
- Characterization and QC of purified antibodies by UV-VIS spectroscopy, Iso-Electric Focusing, and Isotyping etc.
- Determined potency/activity of antibodies and proteins using suitable cell-based assays.
- Phycoerythrin, biotin conjugation of antibodies for use in immunoassays and flow cytometry applications.
- Recognized for outstanding performance with an Employee Rating of 4.1/5, consistently exceeding expectations.
Formulations Chemist
Abbott Laboratories
San Diego, CA
03.2019 - 05.2021
- Operated wide range of laboratory equipment, including pH meters, centrifuges, analytical balances, spectrophotometers, and vacuum chambers, ensuring precision in all research activities.
- Utilized advanced systems such as tangential flow filtration, membrane printing, and conjugate dispensing to enhance research outcomes.
- Expertly prepared intricate solutions, encompassing phosphate buffers, antigen conjugates, antibody conjugates, and gold-antibody conjugates, adhering to strict quality standards.
- Led optimization of gold-antibody conjugate pad formulations, enhancing performance and reliability.
- Conducted rigorous testing on gold-antibody conjugate pad samples, utilizing both drug-positive and drug-negative urine to ensure comprehensive results.
- Undertook complex experimentation, encompassing data collection, summarization, and in-depth analysis, driving product improvement and validation projects.
Undergraduate Student Researcher
Kavli Institute For Brain & Mind
San Diego, CA
06.2017 - 08.2018
- Investigating how alternative methods can be employ to create site-directed mutagenesis.
- Polymerase
Chain Reaction and other techniques
designed to introduce mutations into cloned genes
Education
B.S - Biochemistry & Cell Biology
University of California San Diego
San Diego, CA12.2018
A.S - Biological Studies
San Diego Miramar College
San Diego, CA05.2015
Skills
- Laboratory: Human plasma and serum preparation from whole Blood, Protein Dialysis, Lateral Flow immunoassay (LFIA), Tangential flow filtration (TFF), Flow cytometry (FACS), Centrifugation, Bioassay, Antibody reduction and conjugation methods, SEC, IEX, FPLC and Affinity chromatography on Gilson Akta system, western blot, IEF, UV-VIS spectrophotometry, Microscopy *Computational: JMP14, and SFT12356A , Agile, Smart Solve, Beckman Coulter Kaluza, Microsoft Office 365
Presentations
Chea A, Lucia. Kavli Institute for Brain and Mind with Academic Research Internship Program (AIP)
Understanding the relevant genes in facilitating the learning and memory behaviors of fruit flies, specifically how they learn to not work, UC San Diego, pp. 1-12 (2018).
Chea A. Lucia. Alternative methods to create site-directed mutagenesis by using PCR and other sub-experiments, UC San Diego, pp. 1-12 (2017)
Investigating how alternative methods can be employ to create site-directed mutagenesis by using Polymerase Chain Reaction and other techniques designed to introduce mutations into cloned genes.
Skills
approach, biology,, GC, IEF, Iso, marketing, exchange, Microsoft Office Suite, Optimization, quality control, real-time, research, Preparation of sample, preparing sample, troubleshoot, UV, Demonstrate drive and commitment, Work efficiently and effectively, Team membership, Resilient, Adapt and learn quickly
Work Availability
monday
tuesday
wednesday
thursday
friday
saturday
sunday
morning
afternoon
evening
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Quote
I cannot believe the purpose of life is to be happy. I think the purpose of life is to be useful, to be responsible, to be compassionate. It is, above all, to matter, to count, to stand for something, to have made some difference that you lived at all.
Leo Rosten
Timeline
Biochemist II
Revvity (BioLegend)
05.2021 - Current
Formulations Chemist
Abbott Laboratories
03.2019 - 05.2021
Undergraduate Student Researcher
Kavli Institute For Brain & Mind
06.2017 - 08.2018
B.S - Biochemistry & Cell Biology
University of California San Diego
A.S - Biological Studies
San Diego Miramar College
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