Luciana Relly

Internationally recognized expert in animal biotechnology and genetic engineering of livestock with a documented track record of success in biomedical science research. Innovator with proven ability to establish and manage collaborative research projects connecting academic and corporate partners. Experience in US and Brazilian biotechnology regulatory processes. Seeking to utilize my knowledge and experience to advance biomedical sciences in a progressive corporate environment.

• Rodrigues Willhelm B, Ticiani E, Campagnolo K, Borba G, de Mattos K, Bello CAP, Ongaratto FL, Rodriguez-Villamil P, Relly L, Alves JPM, Rondina D, Rigo Rodrigues JL, Bertolini M. Promoter-specific expression of the imprinted IGF2 gene in bovine oocytes and preimplantation embryos. Reprod Domest Anim. 2021 Mar 13. doi: 10.1111/rda.13925. Epub ahead of print. PMID: 33713470.
• Santos MJS, Canuto KM, de Aquino CC, Martins CS, Brito GAC, Pessoa TMRP, Bertolini LR, de Sá Carneiro I, Pinto DV, Nascimento JCR, da Silva BB, Valença JT Jr, Guedes MIF, Owen JS, Oriá RB. A Brazilian regional basic diet- induced chronic malnutrition drives liver inflammation with higher ApoA-I activity in C57BL6J mice. Braz J Med Biol Res. 2020;53(6): e9031. doi: 10.1590/1414-431x20209031.
• Lima AAM, Oliveira DB, Quetz JS, Havt A, Prata MMG, Lima IFN, Soares AM, Filho JQ, Lima NL, Medeiros PHQS, Santos AKS, Veras HN, Gondim RNDG, Pankov RC, Bona MD, Rodrigues FAP, Moreira RA, Moreira ACOM, Bertolini M, Bertolini LR, Freitas VJF, Houpt ER, Guerrant RL. Etiology and severity of diarrheal diseases in infants at the semiarid region of Brazil: A case-control study. PLoS Negl Trop Dis. 2019, 13(2): e0007154
• Costa Gerger RPD, Souza Ribeiro E, Zago FC, Aguiar LH, Rodriguez-Villamil P, Ongaratto FL, Ambrósio CE, Miglino MA, Rodrigues JL, Forell F, Bertolini LR, Bertolini. Effects of fusion-activation interval and embryo aggregation on in vitro and in vivo development of bovine cloned embryos. M. Res Vet Sci. 2019 123:91-98. doi: 10.1016/j.rvsc.2018.12.001.Paz MP, de Sousa FGC, Alves BG, Lobo CH, Sales AD, Tavares KCS, de Sá NAR, Guerreiro DD, Maside C, Rocha RMP, Bertolini LR, Bordignon V, de Figueiredo JR, Rodrigues APR. Effect of aquaporin 3 knockdown by RNA interference on antrum formation in sheep secondary follicles cultured in vitro. Zygote 2018, 26(5):350-358.
• Fernandes CCL, Aguiar LH, Calderón CEM, Silva AM, Alves JPM, Rossetto R, Bertolini LR, Bertolini M, Rondina D. Nutritional impact on gene expression and competence of oocytes used to support embryo development and livebirth by cloning procedures in goats. Anim Reprod Sci. 2018,188:1-12.
• Carneiro IS, Menezes JNR, Maia JA, Miranda AM, Oliveira VBS, Murray JD, Maga EA, Bertolini M, Bertolini LR. Milk from transgenic goat expressing human lysozyme for recovery and treatment of gastrointestinal pathogens. Eur J Pharm Sci. 2018, 15,112:79-86.
• Costa Gerger RPD, Souza Ribeiro E, Zago FC, Aguiar LH, Rodriguez-Villamil P, Ongaratto FL, Ambrósio CE, Miglino MA, Rodrigues JL, Forell F, Bertolini LR, Bertolini M. Effects of fusion-activation interval and embryo aggregation on in vitro and in vivo development of bovine cloned embryos. Res Vet Sci. 2018, 15;123:91-98.
• Aguiar LH, Ticiani E, Rodriguez-Villamil P, Ongaratto FL, Lazarotto CR, Rodrigues JL, Bertolini LR, Bertolini M. Probability, odds and random chance: the difficult task of modulating the epigenetic profile of cloned embryos. Animal Reproduction 2017, 14:102-123.
• Gerger RPC, Rossetto R, Ribeiro ES, Ortigari Jr. I, Zago FC, Aguiar LH, Willhelm BR, Sanabria GH, Mentz DA, Costa UM, Lopes RFF, Ambrósio CE, Miglino MA, Rodrigues JL, Forell F, Bertolini LR, Bertolini M. Impact of cummulative gain in expertise on the efficiency of Handmade Cloning in cattle. Theriogenology 2017, 95:24-32.
• Gerger RPC, Zago F, Ribeiro ES, Gaudencio Neto S, Martins LT, Aguiar LH, Rodrigues VHV, Furlan FH, Ortigari I Jr, Sainz RD, Ferrell CL, Miglino MA, Ambrosio CE, Rodrigues JL, Rossetto R, Forell F, Bertolini LR, Bertolini M. Morphometric developmental pattern of bovine Handmade Cloned concepti in late pregnancy. Reproduction, Fertility and Development 2017, 29(5):950-967.
• Martins LT, Gaudencio Neto S, Tavares KCS, Calderon CEM, Aguiar LH, Lazzarotto C, Ongaratto FL, Rodrigues VHV, Carneiro IS, Rossetto R, Almeida AP, Fernandes CCL, Rondina D, Dias ACO, Chies JM, Polejaeva I, Rodrigues JL, Forell F, Bertolini LR, Bertolini M. Developmental outcome and related abnormalities in goats: comparison between somatic cell nuclear transfer- and in vivo -derived concepti during pregnancy through term. Cellular Reprogramming 2016, 18:264-279.
• Bertolini LR, Meade H, Lazzarotto CR, Martins LT, Tavares KCS, Bertolini M, Murray JD. Prospective and perspective views for the use of the transgenic animal platform for biopharming. Transgenic Research 2016, 25:329-343.
• Garas LC, Feltrin C, Hamilton MK, Hagey JV, Murray JD, Bertolini LR, Bertolini M, Raybould HE, Maga EA. Milk with and without lactoferrin can influence intestinal damage in a pig model of malnutrition. Food & Function 2016, 7:665- 678.
• Tavares KCS, Carneiro IS, Rios DB, Feltrin C, Ribeiro AK, Gaudencio Neto S, Martins LT, Lazzarotto C, Calderon CEM, Lopes FEM, Teixeira LPR, Bertolini M, Bertolini LR. A fast and simple method for the PCR-based sexing of livestock embryos. Genetics and Molecular Research 2016, 22:15
• Tavares KCS, Dias ACO, Lazzarotto CR, Gaudencio Neto S, Carneiro IS, Ongaratto FL, Pinto AFM, Aguiar LH, Calderon CEM, Toledo Jr, Castro FO, Santos DS, Chies JM, Bertolini M, Bertolini LR. Transient expression of functional glucocerebrosidase for treatment of Gaucher’s Disease in the goat mammary gland. Molecular Biotechnology 2016, 58:47-55.
• Fernandes CCL, Martins LT, Gaudêncio Neto S, Tavares KCS, Aguiar LH, Calderón CEM, Silva AM, Alves JPM, Silva CMG, Rossetto R, Bertolini LR, Bertolini M, Rondina D. Gene expression, oocyte quality and embryo production by cloning in goats supplemented with different diets. Small Ruminant Research 2016, 144:255-262.
• Alves JPM, Fernandes CCL, Lazzarotto CR, Aguiar LH, Calderon CEM, Tavares KCS, Bertolini LR, Bertolini M, Rondina D. Comparative expression profiles of genes related to cellular stress and development in oocyte of goats fed with distinct concentrations of lipids. Acta Scientiae Veterinariae 2015, 43:1296.
• Araújo CV, Lazzarotto CR, Aquino CC, Figueiredo IL, Costa TB, Oliveira Alves LA, Ribeiro RA, Bertolini LR, Lima AAM, Brito GAC, Oriá RB. Alanyl-glutamine attenuates 5-fluorouracil-induced intestinal mucositis in apolipoprotein E- deficient mice. Brazilian Journal of Medical and Biological Research 2015, 48:493-501.
• Alves JPM, Bertolini M, Bertolini LR, Silva CMG, Rondina D. Lipotoxicity: impact on oocyte quality and reproductive efficiency in mammals. Animal Reproduction 2015, 12:291-297.
• Martins LT, Tavares KCS, Teixeira LPR, Lopes FEM, Gaudencio Neto S, Calderon CEM, Aguiar LH, Carneiro IS, Lazzarotto CR, Bertolini M, Bertolini LR. Production of recombinant therapeutic proteins in genetically engineered animals: the dawn of a new era. Acta Scientiae Veterinariae 2015, 43:1-27. [Portuguese]
• Feltrin C, Cooper CA, Mohamad-Fauzi N, Rodrigues VHV, Aguiar LH, Gaudencio- Neto S, Martins LT, Calderón CEM, Morais AS, Carneiro IS, Almeida TM, Silva ING, Rodrigues JL, Maga EA, Murray JD, Libório AB, Bertolini LR, Bertolini M. Systemic immunosuppression by methylprednisolone and pregnancy rates in goats undergoing the transfer of cloned embryos. Reproduction in Domestic Animals 2014, 49:648-656.
• Silva LM, Lazzarotto C, Tavares KCS, Oliveira CHA, Silva AM, Fernandes CCL, Aguiar LH, Martins LT, Gaudencio Neto S, Rios DB, Bertolini LR, Bertolini M, Silva CMG, Rondina D. Comparative expression profiles of genes related to oocyte development in goats after long-term feeding with biodiesel castor industry residues. Animal Reproduction Science 2014, 148:32-41.
• Silva LM, Oliveira CHA, Silva A, Fernandes CCL, Duarte SS, Lima IMT, Silva CMG, Martins LT, Calderon CEM, Tavares KCS, Lazzarotto C, Bertolini LR, Bertolini M, Rondina D. Gene expression and embryo quality in superovulated goats supplemented with crude glycerin after mating. Small Ruminant Research 2014, 120:71-77.
• Pereira AF, Feltrin C, Almeida KC, Carneiro IS, Avelar SRG, Alcântara Neto AS, Sousa FC, Melo CHS, Moura RR, Teixeira DIA, Freitas VJF, Bertolini LR, Bertolini M. Analysis of factors contributing to the efficiency of the in vitro production of transgenic goat embryos (Capra hircus) by handmade cloning (HMC). Small Ruminant Research 2013, 109:163-172.
• Bertolini LR, Feltrin C, Gaudencio Neto S, Martins LT, Tavares KCS, Rodrigues, VHV, Aguiar LH, Calderon CEM, Almeida JL, Bertolini M. Animal cloning: survival of the fittest. Ciencia Animal 2012, 22:82-105. [Portuguese]
• Vieira FK, Forell F, Gerger RPC, Aguiar LH, Feltrin C, Gaudencio Neto S, Calderon CEM, Carneiro IS, Bressan FF, Urio M, Costa UM, Bertolini LR, Meirelles FV, Bertolini M. In vitro development and cell allocation after aggregation of syngeneic wild type and fluorescence-expressing bovine cloned embryos. Acta Scientiae Veterinariae 2012, 40:1057.
• Carvalho EB, Maga EA, Quetz J, Lima IFN, Magalhaes HYF, Rodrigues FAR, Silva AVA, Prata MMG, Cavalcante PA, Havt A, Bertolini M, Bertolini LR, Lima AAM. Goat milk with and without increased concentrations of lysozyme improves repair of intestinal cell damage induced by enteroaggregative Escherichia coli. BMC Gastroenterology 2012, 12:106.
• Tavares KCS, Pinho RM, Carneiro IS, Aguiar LH, Calderon CEM, Martins LT, Ambrósio CE, Bertolini M, Maga EA, Murray JD, Bertolini LR. Efficient RNAi-induced protein knockdown in somatic cells using diced or chemically produced Small Interfering RNAs (siRNA). Acta Scientiae Veterinariae 2012, 40:1048.
• Ambrosio CE, Wenceslau CV, Nogueira JL, Abreu DK, Rodrigues EAF, Lessa LB, Martins DS, Bertolini LR, Miglino MA. Fetal membranes stem cells application in pets. Acta Scientiae Veterinariae 2011, 39:97-101.
• Almeida KC, Pereira AF, Alcântara Neto AS, Bertolini LR, Bertolini M, Freitas VJF, Melo LM. Real-time qRT-PCR analysis of EGF receptor in cumulus- oocyte complexes recovered by laparoscopy in hormonally treated goats. Zygote 2010, 27:1-10.
• Gerger RPC, Ribeiro ES, Forell F, Bertolini LR, Rodrigues JL, Ambrosio CE, Miglino MA, Mezzalira A, Bertolini M. In vitro development of cloned bovine embryos produced by handmade cloning using somatic cells from distinct levels of cell culture confluence. Genetics and Molecular Research 2010, 9:295-302.
• Ribeiro ES, Gerger RPC, Ohlweiller LU, Ortigari I, Mezzalira JC, Forell F, Bertolini LR, Rodrigues JL, Ambrosio CE, Miglino MA, Mezzalira A, Bertolini M. Developmental potential of bovine Hand-Made clone embryos reconstructed by aggregation or fusion with distinct cytoplasmic volumes. Cloning & Stem Cells 2009, 11:377-386.
• Bertolini M, Bertolini LR. Advances in reproductive technologies in cattle: from artificial insemination to cloning. Revista de la Facultad de Medicina Veterinaria y Zootecnia 2009, 56:184-194.
• Bertolini LR, Bertolini M, Maga EA, Madden KR, Murray JD. Increased gene targeting in Ku70 and Xrcc4 transiently deficient human somatic cells. Molecular Biotechnology 2009, 41:106-114.
• Bertolini M, Bertolini LR, Gerger RPC, Batchelder CA, Anderson GB. Developmental problems during pregnancy after in vitro embryo manipulations. Revista Brasileira de Reprodução Animal 2007, 31:391-405.
• Bertolini LR, Bertolini M, Anderson GB, Maga EA, Madden KR, Murray JD. Transient depletion of Ku70 and Xrcc4 by RNAi as a means to manipulate the non-homologous end-joining pathway. Journal of Biotechnology 2007, 128:246- 257.
• Oliveira WG, Teixeira DG, Morini AC, Morini JC, Ambrosio CE, Martins DS, Bertolini LB, Miglino MA, Prada ILS. Estudo anatômico do cerebelo do macaco-prego (Cebus apella Linnaeus, 1758). Biotemas 2007, 20:49-58.
• Souza MF, Assis neto AC, Miglino MA, Bertolini LR, Ambrosio CE, Mariano AN. Disposicao e morfologia externa dos pelos de uma lontra femea da especie Lutra longicaudis (Olfers, 1818) (Carnivore, Mustelidae). Biotemas 2007, 20:73-82.
• Bertolini M, Moyer AL, Mason JB, Batchelder CA, Hoffert KA, Bertolini LR, Carneiro GF, Cargill SL, Famula TR, CC Calvert, Sainz RD, Anderson GB. Evidence of increased substrate availability to in vitro derived bovine fetuses and association with accelerated conceptus growth.eproduction 2004, 128:341-354.
• Andrade MR, Penedo MT, Ward T, Silva VF, Bertolini LR, Roberts JA, Leite JP and Cabello PH. Determination of genetic status in a closed colony of rhesus monkeys (Macaca mulatta). Primates 2004, 45:183-6.
• Bertolini M, Beam SW, Shim H, Bertolini LR, Moyer AL, Famula TR, Anderson GB. Growth, development and gene expression by in vivo - and in vitro - produced day-7 and day-16 bovine embryos. Molecular Reproduction and Development 2002, 63:318-328.
• Chen LR, Shiue YL, Bertolini LR, Medrano JF, BonDurant RH and Anderson G.B. Establishment of pluripotent cell lines from porcine preimplantation embryos. Theriogenology. 1999, 52:195-212.