Luke Berry
Apex,NC
Summary
Accomplished Scientist with a robust background in analytical method development and troubleshooting, honed at KBI Biopharma. Expert in laboratory techniques like LC-MS and proficient in interdepartmental communication, led over 47 projects to successful completion, ensuring compliance and enhancing client satisfaction through innovative solutions and effective team mentorship.
Overview
5
5
years of professional experience
Work History
Scientist II
KBI Biopharma
07.2023 - Current
- Oversaw the development and transfer of methods used to determine large molecule purity and characterization assays (IEX, SEC, RP, HIC, Titer, CGE-SDS, icIEF, residuals, HTRF, ELISA Potency, Ellman's, N-Glycan, Peptide Mapping, LC-MS) for over 12 projects
- Coordinated between clients and GMP manufacturing groups about the status of pending methods and aligned on paths forward if any issues occurred.
- Mentored Scientist I's and Research Associates on how to design and troubleshoot development experiments
- Ensured compliance of Scientist I's and research associates with trackers, dashboards, and functional area workflows
- Reviewed results and technical documents for data integrity and corrections (transcription errors, flagging out of trend results, technical troubleshooting, report corrections, etc.) prior to sharing information with clients
- Proper escalation of method issues and timeline delays that could cause complications with Method Qualifications and GMP manufacturing
- Developed comprehensive training modules and served as lead trainer for LC-MS based methods, including N-Glycan and Peptide Map Analysis
- Assisted in the development of a comprehensive training modules and served as a lead trainer for based methods, including SEC, Titer, IEX, RP, and HIC
- Maintained accurate records of all methods and research conducted and wrote in-depth reports.
Scientist I
KBI Biopharma
05.2021 - 07.2023
- Developed, transferred, and prequalified analytical methods for large molecule purity and characterization assays (IEX, SEC, RP, HIC, CGE-SDS and icIEF) for over 35 projects
- Authored method development (MD)/establishment reports, analytical test methods, and method development presentations for clients and GMP manufacturing
- Authored MD guides to assist troubleshooting and development for challenging molecules
- Served as technical lead for Waters Xevo G2-XS LC-MS and Waters H-Class UPLC instrumentation (served as primary troubleshooter and kept current on new instrumentation)
- Aligned data processing and reporting among Analytical Development teams through use of custom fields and data templates
- Served as a continuous improvement representative to suggest and implement internal improvement
- Served as lead contact for hazardous waste disposal group, ensuring the proper and timely disposal of waste products.
- Assisted in the training and troubleshooting of methods with Research Associates to ensure compliance and increase frequency of right first time completion.
Postdoctoral Researcher Associate
University Of Nebraska Lincoln
06.2020 - 05.2021
- Worked in the lab of Associate Professor Toshihiro Obata
- Conducted independent research focused on plant metabolomics to identify small molecules involved in drought resistance
- Operated and maintained Agilent GC-MS instrument to ensure acquisition of accurate data.
- Assisted and trained graduate and undergraduate research associates in the design of experiments, operation of instrumentation, and data analysis
- Served as lab manager to ensure key reagents and supplies are stocked to prevent delays in testing
Postdoctoral Research Associate
University of Nebraska Lincoln
06.2019 - 06.2020
- Worked in the lab of Jiri Adamec, PhD
- Responsible for the operation and maintenance of a Bruker Solarix FT-ICR LC-MS and Bruker Compact QqTOF
- Assisted fellow postdoctoral research associates, graduate students, and undergraduates in the operation and acquisition of data using LC-MS instruments
- Participated in training at Bruker Daltonics in Billerica, MA to learn about MALDI MS imaging
- Implemented protocols for MALDI MS Imaging
- Collaborated with multiple labs on campus to design LC-MS methods for the acquisition and analysis of a variety of data types including MRM, proteomics, peptide mapping, and intact mass analysis
Education
Ph.D. - Biochemsitry
Montana State University - Bozeman
Bozeman, MT05.2019
Skills
- Laboratory techniques (HPLC, UPLC, CGE-SDS, SEC, icIEF, RP, HIC, SoloVPE, Potency, N-Glycan, Peptide Mapping, LC-MS)
- Software Operation (Waters Empower, Waters MassLynx, Softmax Pro, Electronic Laboratory Notebook)
- Technical Document Drafting
- Analytical Method Development and Troubleshooting
- Interdepartmental Communication
- Compliance with safety and data integrity requirements
- Management of multiple projects and communication with clients
Publications
Tokmina-Lukaszewska, M., Huang, Q., Berry, L., Kallas, H., Peters, J.W., Seefeldt, L. C., Raugei, S., and Bothner, B. (2023). "Fe protein docking transduces conformational changes to MoFe nitrogenase active site in a nucleotide-dependent manner." Communications Chemistry 6(1): 254.
Sun, G., Wase, N., Shu, S., Jenkins, J., Zhou, B., Torres-Rodriguez, J. V., Chen, C., Sandor, L., Plott, C., Yoshinga, Y., Daum, C., Qi, P., Barry, K., Lipzen, A., Berry, L., Pedersen, C., Gottilla, T., Foltz, A., Yu, H., O'Malley, R., Zhang, C., Devos, K. M., Sigmon, B., Yu, B., Obata, T., Schmutz, J., and Schnable, J. C. (2022). "Genome of Paspalum vaginatum and the role of trehalose mediated autophagy in increasing maize biomass." Nature Communications 13(1): 7731.
Lakshmanan, R. V., Hull, J.A., Berry, L., Burg, M., Bothner, B., McKenna, R., and Agbandje-McKenna, M. (2022). "Structural dynamics and activity of B19V VP1u during the pHs of cell entry and endosomal trafficking." Viruses 14(9): 1922.
Sun, G., Wase, N., Shu, S., Jenkins, J., Zhou, B., Chen, C., Sandor, L., Plott, C., Yoshinga, Y., Daum, C., Qi, P., Barry, K., Lipzen, A., Berry, L., Gottilla, T., Foltz, A., Yu, H., O'Malley, R., Zhang, C., Devos, K. M., Sigmon, B., Yu, B., Obata, T., Schmutz, J., and Schnable, J. C. (2021). "The genome of stress tolerant crop wild relative Paspalum vaginatum leads to increased biomass productivity in the crop Zea mays." bioRxiv: 2021.2008. 2018.456832.
Walsh, D. J., Livinghouse, T., Durling, G. M., Arnold, A D., Brasier, W., Berry, L., Goeres, D. M., and Stewart, P. S. (2021). "Novel phenolic antimicrobials enhanced activity of iminodiacetate prodrugs against biofilm and planktonic bacteria." Chemical Biology & Drug Design 97(1): 134-147.
Berry, L., Patterson, A., Pence, N., Peters, J.W., and Bothner, B. (2018). "Hydrogen deuterium exchange mass spectrometry of oxygen sensitive proteins." Bio-protocol 8(6): e2769-e2769.
Berry, L., Poudel, S., Tokmina-Lukaszewska, M., Colman, D.R., Nguyen, D.M., Schut, G.J., Adams, M.W.W., Peters, J.W., Boyd, E.S., and Bothner, B. (2018). "H/D exchange mass spectrometry and statistical coupling analysis reveal a role for allostery in a ferredoxin-dependent bifurcating transhydrogenase catalytic cycle." Biochimica et Biophysica Acta (BBA)-General Subjects 1862(1): 9-17.
Tokmina-Lukaszewska, M., Patterson, A., Berry, L., Scott, L., Balasubramanian, N., and Bothner, B. (2018). "The role of mass spectrometry in structural studies of flavin-based electron bifurcating enzymes." Frontiers in microbiology 9: 1397. van Erp, P. B., Patterson, A., Kant, R., Berry, L., Golden, S. M., Forsman, B. L., Carter, J., Jackson, R. N., Bothner, B., and Widenheft, B. (2018). "Conformational dynamics of DNA binding and Cas3 recruitment by the CRISPR RNA-guided cascade complex." ACS chemical biology 13(2): 481-490.
Lubner, C. E., Jennings, D. P., Mulder, D. W., Schut, G. J., Zadvornyy, O.A., Hoben, J.P., Tokmina-Lukaszewska, M., Berry, L., Nguyen, D. M., Lipscomb, G. L., Bothner, B., Jones, A. K., Miller, A. F., King, P. W., Adams, M. W. W., and Peters, J. W. (2017). "Mechanistic insights into energy conservation by flavin-based electron bifurcation." Nature chemical biology 13(6): 655-659.
Instrumentation
- Bruker Daltonics Solarix 7.5 T FT-ICR
- Bruker Daltonics Compact QqTOF
- Bruker Daltonics Autoflex III Smartbeam MALDI-TOF/TOF
- Bruker Daltonics micrOTOF ESI-Q-TOF
- Agilent Technologies 6410 Triple Quadrupole
- Agilent Technologies 6538 Ultra High Definition Accurate-Mass ESI-Q-TOF
- Agilent Technologies 1290 Infinity UPLC
- Agilent Technologies 7200 Accurate Mass Q-TOF GC/MS
- Thermo Scientific Dionex Ultimate 3000 Nano LC
- Waters H-Class UPLC
- Waters Xevo G2-XS Q-TOF
Software
- Bruker Daltonics: HyStar, DataAnalysis, ApexControl, TOF Control, flexControl, flexAnalysis, and flexImaging.
- Agilent Technologies: Qualitative Analysis and Quantitative Analysis
- Sierra Analytics: HDExaminer
- UCSF Chimera.
- Waters Empower
- Waters MassLynx
- Waters Biopharmalynx
- SoftMax Pro
- Electronic Laboratory Notebook (ELN)
Timeline
Scientist II
KBI Biopharma
07.2023 - Current
Scientist I
KBI Biopharma
05.2021 - 07.2023
Postdoctoral Researcher Associate
University Of Nebraska Lincoln
06.2020 - 05.2021
Postdoctoral Research Associate
University of Nebraska Lincoln
06.2019 - 06.2020
Ph.D. - Biochemsitry
Montana State University - Bozeman
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