MAHMOUD ALHAJ, MB (ASCP) CM

● Perform Ion Torrent NGS testing for Oncomine & Myeloseq Focus Gene Panel for detection of mutations, copy number gains, and gene fusions in 50 cancer genes.
● Perform NYU Langone Genome PACT (Profiling of Actionable Cancer Targets), to detect tumor gene alterations in exons and selected promoter of 607 genes.
●Perform Quantitative PCR for BCR/ABL1 p210 transcript (international scale), using 7500 DX qPCR, and BCR/ABL1 p190.
●Testing Acute myeloid leukemia: FLT3-ITD, FLT3-TKD mutations, PML/RARA fusions t(15;17), RUNX1/RUNX1T1 fusion t(8;21).
●Testing Myeloproliferative neoplasms single gene mutations: JAK2 V617F mutation, Calreticulin mutations (CALR), T- and B-cell clonality assay.
●Testing Lymphoma: IGH/CCND1 t(11;14)(CYCLIN D1) fusion, IGH/BCL2 t(14;18) fusion, EBV, HTLV1, HHV8.
●Perform 1p19q Loss of Heterozygosity for diagnosis of oligodendroglioma and predicting chemosensitivity.
●Perform Prosigna for 50 gene RNA expression profiling of invasive breast cancer using PAM-50 prediction score.
●Perform MSI PCR for Microsatellite instability with mismatch repair and as a biomarker for response to immunotherapy.
●Perform Pyrosequencing for MGMT Glicomas.
●Perform Covid 19, HPV and CT/NG Viruses detection using Cobas 4800 from Roche, and Cobas SARS-Co-V-2 using Cobas 6800 system.
●Perform Routine real-time PCR molecular testing and data analysis using 3500 XL Genetic Analyzer.
●Perform DNA, RNA and Plasma extraction from various specimen types, using either manual or automated methods using Promega Maxwell RSC, Qiacube and EZ1).
●Involve in Mixes preparation, Troubleshooting, Instruments calibration, validation & development of new assays, and training new staff on various assays & procedures following the SOP.

●Perform daily Nucleic acid extraction for DNA/ RNA from various specimen types, using either manual or automated methods.
● Run Routine PCR assays, qPCR, RT-PCR and NGS assays.
● Validate new assays, help in writing protocols and submit to NYS for approval.

• Assistance at Nephrology laboratory at Massachusetts General Hospital and Harvard Medical School working on Stem Cells based Therapy project on regulation of TGFβ Signaling by Stem Cell Antigen-1 in Acute Kidney Injury, and also study the role of Sca-1 after ischemia/reperfusion injury in adult mice.
  ●Worked both independently and collaboratively in fast-paced Harvard Medical School laboratory environment.
  ●Attended seminars and symposiums to improve overall knowledge and understanding like Experimental Biology Conference in Boston, MA.

• Perform Genomic DNA extraction, RT PCR and agarose gel analysis for the purposes of genotyping knockout and transgenic mouse.
  ● Perform Immunofluorescent antibody staining of frozen tissue sections used for both Confocal microscopy and fluorescent slide scanning.

●Operate regular domestic/ international flights from and to Saudi Arabia, mainly for Hajj and Umrah.
●Ensured safety and comfort of customers onboard aircraft.
●Verified aircraft and passenger compliance with policies, regulations, and safety guidance.
●Resolved passenger conflicts and medical emergencies during flights.
●Facilitated communication between flight deck and cabin crew prior to and during flights to promote smooth operations.
●Clearly explained and demonstrated safety and emergency procedures to passengers prior to takeoff.
●Attended workshops and trainings in customer service, conflict resolution tactics, and safety procedures to keep abreast of all new requirements and procedures.
●Greeted passengers, assisted with carry-on baggage storage and delivered onboard announcements.
●Performed pre-flight safety checks to verify oxygen masks, life jackets and other emergency equipment in good working order.

Working in Blood Bank and Molecular Lab to help extract the DNA/RNA and set up the Agarose gel and SDS/PAGE for protein analysis.

• Stem Cell Training on Magnetic separation of Stem Cells for research & clinical applications, and trained on CliniMACS and AutoMACS pro purification systems for Stem Cells.

• Trained and worked in a research project (Surveillance Study of Small ruminant Lentivirus infection in large animals in Jordan) by prepare the samples, extract the genomic DNA and test it with visitor professor from Pisa University- Italy.

• Trained and worked In a medical laboratory sciences from Routine Blood Bank to Immunology to Endocrinology and BioChemistry and Forensics Science.