Marie Lomax
Hockley,TX
Summary
Medical Microbiologist & Molecular Geneticist with over 30 years of expertise conducting microbiological testing protocols and analyzing genetic data generated through whole genome sequencing. Skilled in laboratory management of equipment and of CAP Procedures, maintaining daily logs, corrective action logs, equipment record bookkeeping, training and supervising staff, ordering of supplies and budget. Efficiently manage daily routines of DNA extraction and isolation of organisms, proficient in MALDI and STREK testing. Design and program PCR, pour and perform gel electrophoresis. Tape station DNA analysis of both genomic and DNA libraries. Maintain growth media, testing of media, gram staining, isolation of cultures from patient samples presented weekly to clinic. WGS of foodborne illness, HAI, STEC, COVID-SARS and Waste Water using Illumina MiSeq technology. Preparation of laboratory materials and solutions, SOP compliant. In addition to being a Secondary Composite Certified Science Educator and Curriculum developer, designer of differentiated hands on lab experience for all learners. Specializing in Advanced Placement Biology, Environmental Science, Chemistry, Physics. Biology.
Overview
35
35
years of professional experience
1
1
Certification
Work History
Microbiology Laboratory Technician
CITY OF HOUSTON HEALTH DEPARTMENT
10.2022 - Current
- thePerformance of microbiological testing protocols and analysis of data generated through whole genome sequencing
- Skilled in laboratory management of CAP Procedures, maintaining daily logs, corrective action logs, equipment records.
- Efficiently manage daily routines of DNA extraction, streaking, isolation of organisms, Gram staining, PCR and Gel Electrophoresis testing.
- WGS PulseNet Certified for foodborne illness, HAI, STEC, COVID SARS, and Listeria anlaysis perfomed weekly up to 45 samples.
- Improved sample analysis turnaround time through organization of daily tasks and efficient use of resources.
- Troubleshooted and resolved equipment process issues, cleaned and maintained Miseq equipment.
- Ensured high-quality results by following strict protocols for various microbiological testing procedures including Qubit, Nanodrop, and Tape Station Bioanalysis.
- Prepared samples for analysis by performing SOP for Illumina and Nextera DNA Library Preparations.
- Collaborated with team members to troubleshoot issues and optimize lab operations for successful project completion.
- Assisted in identification and characterization of microorganisms through advanced microscopic techniques such as MALDI.
- Recorded and analyzed data to produce reports of results, weekly use of Clarity LIMS system for both bacterial and viral analysis.
- Prepared reagents and solutions following standard laboratory formulas and procedures.
Microbiologist & Molecular Biology Clinical Lab
Baylor College of Medicine
04.1989 - 06.1993
- Department of Gastroenterology, Supervise and training of staff in Helicobacter pylori identification, growth and identification of clinical patient samples from the VA Hospital
- Gram staining, culture isolation, Immuno-assay, Gel electrophoresis, ELISA testing, maintenance of lab equipment & incubators, media testing and preparation, ordering of supplies, and preparation of solutions.
- DNA Forensic Laboratory, Cystic Fibrosis, DNA isolation, genetic analysis through PCR gel electrophoresis and SDS Gel electrophoresis for patient identification and genotyping, pedigree generation.
- Department of Immunology, PCR identification of p53 mutation and insertion in Hepatitis B positive tumors research, maintenance of p53 mutant mice. Primer generation using DNA sequencer, production of clones and recombinant plasmids.
- Operated genetic analyzer to sequence DNA.
- Enabled successful grant applications by providing accurate experimental data as part of a team effort in securing funding sources for ongoing research projects.
- Maintained all laboratory robotics.
- Provided technical support for the implementation of cutting-edge molecular diagnostic tools such as PCR analyses.
- Assisted in the development of new laboratory protocols, resulting in increased efficiency and cost savings.
- Facilitated research into genetic causes of disease.
- Ordered supplies, supervised and trained three staff members.
- Kept accurate databases of specimens collected and stored in repository.
- Developed innovative assays for rapid pathogen detection, reducing turnaround time for critical diagnostic tests such as blood spot testing for genetic diseases.
- Conducted experiments related to cell culture including DNA isolation from cultured villi samples, and amniocentesis.
- Designed and executed robust experiments to investigate the effects of various environmental factors on microbial growth patterns, protein assays, radiological tracing, tissue cultures.
- Interpreted research findings and summarized data into reports.
- Reduced contamination risk by maintaining a sterile laboratory environment and adhering to safety protocols.
- Extracted DNA and genotype samples using SNP technology.
- Collected and processed specimens for clinical protocols.
- Ensured CAP compliance with regulatory standards by maintaining detailed records of laboratory data and results, temperature and humidity logs.
- Reviewed scholarly articles pertaining to microbiology trends, staying up-to-date on emerging technologies and incorporating them into current methodologies.
Secondary Science Composite Educator & Curriculum Designer
Katy, Fortbend & Waller ISD
08.1995 - 11.2022
- Science Composite Educator with over 25 years of teaching experience
- Secondary ESL Certified, Gifted & Talented Certified
- Facilitated and Designed curriculum for Secondary courses for all levels of learning, ESL, Gifted & Talented, Advanced Placement, Inclusion learners, differentiation of lessons and alignment of curriculum for science courses from kindergarten to Secondary AP Science Curriculum
- Specialize in test writing, interactive learning and science lesson design, analysis of data, immediate feedback design for Biology, Chemistry, AP Biology, AP Environmental Science, Physics.
ETS College Board Grader & Table Leader AP Biology
ETS
06.2005 - Current
- Consult with College and High School Educators to create a grading rubric for the AP Biology College Board Exam
- Training College and High School Educators, managing and supervising 10 educators, back reading, and supporting instructors.
- Managed deadlines effectively, ensuring timely completion of all required tasks while maintaining high-quality standards throughout the evaluation process.
- Developed and maintained courteous and effective working relationships.
- Conducted regular performance reviews to identify areas for improvement and provide constructive feedback.
Educational Consultant and Professional Trainer
NMSI Consulting
08.2006 - 08.2019
- consultantPrepared learning materials for Educators and students across the Nation for success on the AP Biology and AP Environmental Science exams administered through the College Board.
- Trained 100 Secondary Science Teachers through professional learning in AP Biology and Environmental Science as a consultant.
Education
Bachelor of Science - BS - Microbiology
Texas A&M University
College Station, TX12.1988
Master of Arts - Teacher Education
Alternative Teacher Certification
Houston, Texas06.1995
Skills
- Clarity LIMS
- MALDI
- DNA & RNA Extraction
- Specimen Preparation, Plate Streaking and Isolation
- Cell Culture Techniques
- Equipment Calibration
- Enzyme Assays & Protein Purification
- Media Preparation and Testing
- Antibiotic Susceptibility Testing
- Plasmid Isolation
- Asceptic & Sterilization techniques
- Gram Staining
- Illumina Miseq and NexSeq
- ELISA & Immunological assays
- PCR, RFLP, SNP, Cloning, Gel Electrophoresis Techniques
Certification
Secondary Composite Science & English as a Second Language, Certified lifetime, Texas Education Agency
CDC PulseNet Certified WGS Campylobacter, Vibrio, Listeria, E.coli, Salmonella, HAI
MALDI Certified
APHL Bioinformatic Analysis Certified
CPR certified
Honors Awards
- Presidents Leadership Scholarship - McNeese State University, 01/1985
- Houston Dow Chemical Science Excellence Award - Dow, 05/1984
- National Science Honor Society - Alpha Lambda Delta Academic Honor Society, 01/1985
Timeline
Microbiology Laboratory Technician
CITY OF HOUSTON HEALTH DEPARTMENT
10.2022 - Current
Educational Consultant and Professional Trainer
NMSI Consulting
08.2006 - 08.2019
ETS College Board Grader & Table Leader AP Biology
ETS
06.2005 - Current
Secondary Science Composite Educator & Curriculum Designer
Katy, Fortbend & Waller ISD
08.1995 - 11.2022
Microbiologist & Molecular Biology Clinical Lab
Baylor College of Medicine
04.1989 - 06.1993
Bachelor of Science - BS - Microbiology
Texas A&M University
Master of Arts - Teacher Education
Alternative Teacher Certification
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