Mary Gargano
Clinton Township,MI
Summary
Detail-oriented, resourceful, and adaptable Research Assistant with hands-on experience performing clerical and laboratory work to advance research goals. Comfortable handling complex issues and adjusting to rapidly changing conditions.
Overview
39
39
years of professional experience
Work History
Research Assistant
Wayne State University, IEHS/CURES
01.1999 - Current
- Stewardship of Cell Culture Facility: function as a core within the department - provide cell culture services, supplies, training, and sterile environment for IEHS/CURES members. Maintain cell culture and cryo-storage equipment. Work both independently and collaboratively.
- Provide animal procedure services either as a function of the Cell Culture Facility or as a temporary assignment to an alternative lab (mouse and rat hepatocyte isolation, mouse sciatic nerve injection, mouse partial hepatectomy, mouse anesthetic and tissue harvest, Drosophila colony maintenance, Zebra fish care).
- Lab of Dr. Thomas Kocarek: General lab procedures including RNA extraction, reverse transcription, real-time PCR, human primary hepatocyte work.
- Lab of Dr. Melissa Runge-Morris: General lab procedures, human cell line work, Western gel, mouse survival surgery, PCR- based genotyping, dual Luciferase assay.
- For both labs and Cell Culture Facility: Order supplies via Procurement cards and WayneBuy and maintain records, acquire quotes, repair equipment, maintain controlled substance records.
Research Assistant
Wayne State University, IEHS
08.1992 - 01.1999
- Lab of Dr. Ye-Shih Ho: generation of dozens transgenic mouse lines via embryo microinjection and surgical implantation, mouse breeding, DNA isolation and analysis, CAT assay, protein assay and blot of MnSOD, tissue harvest
Lab Animal Technician
Wayne State University, DLAR, DLAR Hospital
12.1984 - 01.1992
- Administered injections, oral medications and topically applied medications prescribed by veterinarian. Prepared animals for surgical procedures.
- Assisted veterinarian during immunologic, medical, surgical, and diagnostic procedures.
- Kept detailed records of animal treatments and medications.
- Cleaned, sterilized and maintained surgical instruments and equipment.
- Cleaned, sterilized and maintained kennels, operating rooms, and surgical equipment.
Licensed Veterinary Technician
Beverly Hills Veterinary Associates
05.1985 - 07.1986
- Educated clients on animal health, preventive care and procedures.
- Provided direct care to patients and assisted with procedures such as radiology, dentistry, critical care and anesthesia. Collected patient biopsies and blood samples for laboratory analysis.
- Prepared surgical instrument packs and drapes for use in sterile environments by processing in autoclave..
- Analyzed bloodwork and sample results.
Education
Bachelor of Arts - Interdisciplinary Studies
Wayne State University
Detroit, MI12.2010
Associate of Applied Science - Veterinary Technology
International Academy Of Macomb
Clinton Township, MI05.1995
Associate of Arts - General Education
Macomb Community College
Warren, MI05.1985
Skills
- Lab Operations, Administrative Duties, Teaching Students
- Biomedical Science, Specimen Handling, Protein Isolation, Conducting Assays, RNA/DNA Extraction
- Lab Safety
- Equipment Management and Maintenance, Managing Laboratory Supplies, Multitasking and Organization
Timeline
Research Assistant
Wayne State University, IEHS/CURES
01.1999 - Current
Research Assistant
Wayne State University, IEHS
08.1992 - 01.1999
Licensed Veterinary Technician
Beverly Hills Veterinary Associates
05.1985 - 07.1986
Lab Animal Technician
Wayne State University, DLAR, DLAR Hospital
12.1984 - 01.1992
Bachelor of Arts - Interdisciplinary Studies
Wayne State University
Associate of Applied Science - Veterinary Technology
International Academy Of Macomb
Associate of Arts - General Education
Macomb Community College
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