Matthew Piron

Doctoral thesis project (Brady Lab)- Metabolic profiling and dietary interventions pertaining to the disease states induced by chronic social isolation induced stress in a mouse model.

2010-2014

• Maintained several animal colony's (mouse). This included: tagging, breeding/weening, housing, feeding and sacrificing animals.
• Genotyped established transgenic animal models. Also designed genotyping protocols for evaluation of in-house (PTG) transgenic mouse model.
• Learned and become proficient in multiple microbiology research techniques: Western blotting, qPCR, luciferase assays, ELISA, sample extractions and preparations (nucleic, protein, lipid and metabolites).
• Mentored a variety of colleagues and students (undergraduate, graduate , and MD research fellows) in microbiology research techniques.

Key Achievements:

Publications Contributed to (non-author):

• Bailey KA, Et Al. (2015) Evidence of non-pancreatic beta cell-dependent roles of Tcf7l2 in regulation of glucose metabolism in mice. Hum Mol Genet 24(6) 1646-54 PMID:25398947
• Carmean CM, Huang YH, Brady MJ. Glycogen Repletion in Brown Adipose Tissue upon Refeeding Is Primarily Driven by Phosphorylation-Independent Mechanisms. PLoS One. 2016 May 23;11(5):e0156148. doi: 10.1371/journal.pone.0156148. PMID: 27213961; PMCID: PMC4877058.

2015-Present:

• Became the principle technician for the Cell Biology Core at the University of Chicago; responsibilities include: Assisting collaborators/clients with experimental design, processing of biological samples, running a wide array of biological assays on users samples, data analysis, and instruction on proper use of Core equipment.
• Trained on metabolic cage use and DEXA scanning of mice.
• Became proficient with Next Gen Sequencing techniques and bioinformatics analysis of gene expression in RNA-seq experiments.
• Trained on use of Immulite 2000 Immuno Assay System.
• Was a teaching assistant for the Endocrinology Specialization (I,II,II) courses at the University of Chicago for several years. Consistently received high ratings by students on post quarter performance reviews.

Key Achievements:

Publications (co-author):

• White JM, Piron MJ, Rangaraj VR, Hanlon EC, Cohen RN, Brady MJ. Reference Gene Optimization for Circadian Gene Expression Analysis in Human Adipose Tissue. J Biol Rhythms. 2020 Feb;35(1):84-97. doi: 10.1177/0748730419883043. Epub 2019 Oct 31. PMID: 31668115; PMCID: PMC7409766.
• Casimiro I, Hanlon EC, White J, De Leon A, Ross R, Moise K, Piron M, Brady MJ. Reduction of IL-6 gene expression in human adipose tissue after sleeve gastrectomy surgery. Obes Sci Pract. 2020 Jan 15;6(2):215-224. doi: 10.1002/osp4.396. PMID: 32313680; PMCID: PMC7156876.
• Goddi A, Carmona A, Schroedl L, White JM, Piron MJ, De Leon A, Casimiro I, Hoffman A, Gonzalez Porras MA, Brey EM, Brady MJ, Cohen RN. Laminin-α4 Is Upregulated in Both Human and Murine Models of Obesity. Front Endocrinol (Lausanne). 2021 Jul
• Vujović N, Piron MJ, Qian J, Chellappa SL, Nedeltcheva A, Barr D, Heng SW, Kerlin K, Srivastav S, Wang W, Shoji B, Garaulet M, Brady MJ, Scheer FAJL. Late isocaloric eating increases hunger, decreases energy expenditure, and modifies metabolic pathways in adults with overweight and obesity. Cell Metab. 2022 Oct 4;34(10):1486-1498.e7. doi: 10.1016/j.cmet.2022.09.007. PMID: 36198293; PMCID: PMC10184753.

• Preformed cell culture work.
• Preformed microscopy profusion experiments that measured intracellular Ca++ flux in store operated calcium channels.
• Preformed electrophysiology experiments via patch-clamp and voltage clamp experiments.
• Was a TA for the course Cancer Biology.

Key Achievement:

Publication (first author):

Piron M, Villereal ML. Chronic exposure to stress hormones alters the subtype of store-operated channels expressed in H19-7 hippocampal neuronal cells. J Cell Physiol. 2013 Jun;228(6):1332-43. doi: 10.1002/jcp.24289. PMID: 23169006.