Michele Larsen
Madison
Summary
Accomplished Scientist with expertise in assay development, data analysis, and laboratory management. Demonstrated success in developing GMP-compliant protocols and mentoring emerging scientists.
Overview
2027
2027
years of professional experience
Work History
Scientist III
University of Wisconsin-Madison
Madison, WI
03.2023 - Current
- Developed GMP-compliant protocols for the isolation of primary human mesenchymal stem cells (MSCs) from labile salivary gland biopsy samples for a regenerative clinical pre-trial.
- Developed protocols for the isolation and differentiation of human monocytes for co-culture experiments with human labile salivary gland MSCs.
- Developed protocols for the isolation of human epithelial cells from labile salivary gland biopsy tissues.
- Developed protocols for the establishment of 3-D organoid cultures from the human epithelial cells.
- Mentored undergraduate and graduate student lab members as well as visiting scientists in laboratory techniques and procedures.
- Maintained the animal breeding colony and associated records.
- Assisted with the initial processing of tissues for the McCoy Lab Biobank.
- Completed all purchasing for the lab and maintained all purchasing records.
Scientist III
University of Wisconsin-Madison
Madison, WI
2021 - 03.2023
- Completed ELISA analyses studying cholesterol mobilization from the plasma membrane or late endosomes for steroid hormone synthesis.
- Maintained IACUC and Biosafety Protocols for Dr. Colin Jefcoate's lab.
- Mentored an undergraduate honors Biochemistry student through her thesis project.
- Closed the lab upon Dr. Jefcoate's retirement.
Senior Scientist
University of Wisconsin-Madison
Madison, WI
2019 - 2021
- Examined the role of a high fat diet in the expression of Cytochrome P450 1B1 (CYP1B1) and its role as a modulator of fatty acid homeostasis in the cell.
- Analyzed the role of CYP1B1 metabolism in polycyclic aromatic hydrocarbon bone marrow toxicity.
- Used Flow Cytometry, real-time PCR and microarray technology to examine the cell-specific targets of toxicity and gene responses.
Associate Scientist
University of Wisconsin-Madison
Madison, WI
2001 - 2019
- Completed studies examining the role of CYP1B1 bioactivation of environmental contaminants in breast cancer initiation.
- Examined bone marrow progenitor suppression mediated by CYP1B1 bioactivation of environmental contaminants.
- Studied human breast tissue epithelial and stromal cell CYP1B1 metabolism with respect to the extracellular matrix environment.
- Established a 3-D breast epithelial organoid model to study the critical role of cellular polarization in Cytochrome P450 expression and function.
Assistant Scientist
University of Wisconsin-Madison
Madison, WI
1997 - 2001
- Examined inter-individual differences in Cytochromes P450 expression in cultured human breast epithelial cells.
- Examined inter-individual differences in Cytochrome P450-mediated polycyclic aromatic hydrocarbon bioactivation in cultured human breast epithelial cells.
- Supervised undergraduate students in research methodologies and practices.
Education
Ph.D. - Chemistry
University of Nebraska
Lincoln1991
Bachelor of Science - Biology, Medical Technology And Chemistry
University of Wisconsin-Superior
Superior1985
Skills
- Student Mentoring
- Assay & Methods Development/Critical Thinking
- Experimental Design
- Quantitative Skills/Data Analysis
- Scientific Writing/Research Publication
- Laboratory Management
- IACUC and Biosafety Protocol Preparation and Management
- Animal Husbandry/Experimental Animal Handling
- Primary Cell Culture (Stem Cells and Multi-Potential Progenitor)/3-D Cell Culture (organoid)/ECM
- Histology (IHC/IF)
- ELISA
- Flow Cytometry
- Large Database Management
Timeline
Scientist III
University of Wisconsin-Madison
03.2023 - Current
Scientist III
University of Wisconsin-Madison
2021 - 03.2023
Senior Scientist
University of Wisconsin-Madison
2019 - 2021
Associate Scientist
University of Wisconsin-Madison
2001 - 2019
Assistant Scientist
University of Wisconsin-Madison
1997 - 2001
Ph.D. - Chemistry
University of Nebraska
Bachelor of Science - Biology, Medical Technology And Chemistry
University of Wisconsin-Superior