Miguel Santos-Fernandez

Passionate and detail-oriented Ph.D. candidate with a strong foundation in mass spectrometry, analytical chemistry, and instrumentation. Seeking a position to apply technical skills and research experience in driving innovation and delivering impactful results. Committed to contributing to a dynamic team and advancing scientific discoveries, while continuously growing expertise and making meaningful contributions to the organization's objectives.

• Santos-Fernandez, M., Jeanne Dit Fouque, K., Zirah, S., Chapagain, P., Hegemann, J.D., Fernandez-Lima, F. (2024). Investigation of the Underlying Mechanism of Lasso Peptide Thermal Unthreading using Liquid Chromatography - Trapped Ion Mobility - Tandem Mass Spectrometry. (Pending Submission)
• Santos-Fernandez, M., Jeanne Dit Fouque, K., Karki, U., Chapagain, P., Fernandez-Lima, F. (2024).Characterization of the lasso and branched-cyclic Syanodin I using Hydrogen-Deuterium Exchange, Trapped Ion Mobility Spectrometry, and tandem Electron Capture Dissociation Mass Spectrometry. (Pending Submission)
• Neupane, D., Santos-Fernandez, M., Fernandez-Lima, F., Meier, K. K. (2024). Multiple Copper Ions Bind to and Promote the Aggregation of Huntingtin Protein with Non-pathological Repeat Expansions. Submitted to ACS Chemical Biology
• Su, L., Deng, Z., Santos-Fernandez, M., Jeanne Dit Fouque, K., Chapagain, P., Chambers, J., Fernandez Lima, F., Leng, F., (2024). Inhibition of HMGA2 Binding to AT-rich DNA by Its Negatively Charged C-Terminus. Submitted to Nucleic Acid Research
• Santos-Fernandez, M., Jeanne Dit Fouque, K., & Fernandez-Lima, F. (2023). Integration of Trapped Ion Mobility Spectrometry and Ultraviolet Photodissociation in a Quadrupolar Ion Trap Mass Spectrometer. Analytical Chemistry., 95(22), 8417–8422. https://doi.org/10.1021/acs.analchem.3c01220
• Jeanne Dit Fouque, K., Hegemann, J. D., Santos-Fernandez, M., Le, T. T., Gomez-Hernandez, M., van der Donk, W. A., & Fernandez-Lima, F. (2021). Exploring structural signatures of the lanthipeptide prochlorosin 2.8 using tandem mass spectrometry and trapped ion mobility-mass spectrometry. Analytical and Bioanalytical Chemistry, 413(19), 4815–4824. https://doi.org/10.1007/s00216-021-03437-x
• Jeanne Dit Fouque, K., Wellmann, M., Leyva Bombuse, D., Santos-Fernandez, M., Cintron-Diaz, Y. L., Gomez-Hernandez, M. E., Kaplan, D., Voinov, V. G., & Fernandez-Lima, F. (2021). Effective discrimination of gas-phase peptide conformers using TIMS-ECD-ToF MS/MS. Analytical methods: advancing methods and applications, 13(43), 5216–5223. https://doi.org/10.1039/d1ay01461g
• Hegemann, J. D., Jeanne Dit Fouque, K., Santos-Fernandez, M., & Fernandez-Lima, F. (2021). A Bifunctional Leader Peptidase/ABC Transporter Protein Is Involved in the Maturation of the Lasso Peptide Cochonodin I from Streptococcus suis. Journal of Natural Products., 84(10), 2683–2691. https://doi.org/10.1021/acs.jnatprod.1c00514
• Le, T., Jeanne Dit Fouque, K., Santos-Fernandez, M., Navo, C. D., Jiménez-Osés, G., Sarksian, R., Fernandez-Lima, F. A., & van der Donk, W. A. (2021). Substrate Sequence Controls Regioselectivity of Lanthionine Formation by ProcM. Journal of the American Chemical Society., 143(44), 18733–18743. https://doi.org/10.1021/jacs.1c09370

• Structural characterization of Syanodin I topoisomers using Hydrogen-Deuterium Exchange, Trapped Ion Mobility Spectrometry, and tandem Electron Capture Dissociation Mass Spectrometry. Miguel Santos-Fernandez, Kevin Jeanne Dit Fouque, Julian D. Hegemann, Ukesh Karki, Prem Chapagain, Francisco Fernandez-Lima. 33rd ISIMS Conference, Miami, Florida, 07/20-26/24, Oral Presentation
• Characterization of the lasso and branched-cyclic Syanodin I using Hydrogen-Deuterium Exchange, Trapped Ion Mobility Spectrometry, and tandem Electron Capture Dissociation Mass Spectrometry. Miguel Santos-Fernandez, Kevin Jeanne Dit Fouque, Julian D. Hegemann, Ukesh Karki, Prem Chapagain, Francisco Fernandez-Lima. 72nd ASMS Conference, Anaheim, California, 06/02-06/24, Poster
• Lasso Peptide Multistep Unthreading by LC-TIMS-UVPD-MS/MS. Miguel Santos-Fernandez, Kevin Jeanne Dit Fouque, Julian D. Hegemann, Francisco Fernandez-Lima. 71st ASMS Conference, Houston, Texas, 06/04-08/23. Poster
• Integration of Trapped Ion Mobility Spectrometry with Quadrupole Ion Traps. Miguel Santos-Fernandez, Kevin Jeanne Dit Fouque, Gordon Anderson, Francisco Fernandez Lima. 98th Florida Annual Meeting and Exposition, ACS FAME, Palm Harbour, Florida, 08/04-08/22. Oral Presentation
• Implementation of a novel Tandem TIMS-QIT-MSn methodology. Miguel Santos-Fernandez, Kevin Jeanne Dit Fouque, Gordon Anderson, Francisco Fernandez Lima. 31st ISIMS Conference, Memphis, Tennessee, 07/24-29/2022, Oral Presentation
• On the coupling of Trapped IMS and QIT MSn. Miguel Santos-Fernandez, Kevin Jeanne Dit Fouque, Gordon Anderson, Francisco Fernandez Lima. 70th ASMS Conference, Minneapolis, Minnesota, 06/05-09/2022. Poster Presentation
• Characterization of Peptide-Peptide complexes using tandem Trapped Ion Mobility, Electron Capture dissociation, and ToF Mass Spectrometry. Miguel Santos-Fernandez, Kevin Jeanne Dit Fouque, Linjia Su, Fenfei Leng, Francisco Fernandez-Lima. 69th ASMS Conference, Philadelphia, Pennsylvania, 10/31/21. Poster Presentation

• Trapped Ion Mobility Spectrometry (TIMS)
• (Nano)-Electrospray Source Ionization (ESI)
• Top-Down Proteomics
• Matrix Assisted Laser Desorption Ionization (MALDI)
• Structure for Lossless Ion Manipulation (SLIM)
• Collision Induced Dissociation (CID)
• Electron Capture Dissociation (ECD)
• Infrared Multiple Photon Dissociation (IRMPD)
• Ultraviolet Photon Dissociation (UVPD)
• Quadrupolar Ion Trap (IT)
• Time-of-Flight (ToF)
• Fourier Transform Ion Cyclotron Resonance (FT-ICR) mass spectrometers
• Infrared Spectroscopy (IR)
• High Performance Liquid Chromatography (HPLC)
• Gas Chromatography (GC)
• Peptide/Protein Purification

• CREST Center for Aquatic Chemistry and Environment Fellowship, 05/01/22 – 06/01/22
• ISIMS Travel Award, 07/01/22

• Google Scholar: https://scholar.google.com/citations?user=C_FshXgAAAAJ&hl=en
• ORCID: https://orcid.org/0000-0003-4233-0203
• Research Gate: https://www.researchgate.net/profile/Miguel-Santos-Fernandez
• Linkedin: https://www.linkedin.com/in/miguel-santos-88a06322b/