Mihika Sharma

Took a deliberate gap year to explore personal interests, focus on health and gain valuable life experiences. During this time:

• Focused on physical and mental well-being
• Learnt and honed skills for handmade products to harness creative skills
• Launched a small business to sell handmade products
• Gained important experience with business, marketing and customer-base
• Contributed to fundraising with knitting for Children's Research
• Developed strong communication and interpersonal skills through interactions with diverse communities

The sabbatical served as a transformative and productive period, providing insights to business, enhancing people skills and giving an outlet to creative side. Excited to bring the gained skills and enriched outlook to new professional opportunities.

• Conducted comprehensive analysis of multi-omics Next-Generation Sequencing (NGS) datasets, both in-house and publicly available, to support various research projects.
• Applied single-cell analysis techniques to investigate ~40 cases of Idiopathic Pulmonary Fibrosis (IPF) and other disease states, aiming to unveil Gene Regulatory Networks (GRNs) for potential drug repurposing strategies.
• Performed transcriptomic analysis on COVID-19 datasets to identify differential regulators implicated in disease progression.
• Employed CellChat and Niche-Net methodologies to elucidate cell-cell communication patterns in IPF and other disease states, comparing them with wild-type conditions.
• Utilized spatial single-cell and transcriptomic analyses in tandem to resolve the spatial-expression complexities of cells within the lung.
• Implemented Weighted Gene Correlation Network Analysis (WGCNA) to identify significant modules of genes involved in expression-based network construction, particularly in Ulcerative Colitis and other disease models.
• Integrated outputs from image analysis to derive gene correlation network regulations, contributing to a comprehensive understanding of cellular interactions and regulatory mechanisms.

• Conducted single-cell RNA sequencing (scRNA-seq) to assess the induction of human stem cells through co-culturing human induced pluripotent stem cells (iPSCs) and mouse embryonic stem cells (ESCs), elucidating the cellular dynamics and gene expression changes involved in this process.
• Employed organoid models to conduct multi-drug screening analyses, aiming to evaluate the efficacy and toxicity of various compounds and identify potential candidates for therapeutic intervention.
• Investigated the lineage diversification within gastro-hepatic cells to develop in vitro model boundary organoids, leveraging single-cell RNA sequencing (scRNA-seq) techniques to characterize cell populations and their differentiation trajectories.
• Analyzed SNP-Chip Microarray data obtained from pooled iPSC samples, identifying subpopulations harboring disease-associated Single Nucleotide Polymorphisms (SNPs) and discerning their potential implications in disease susceptibility and pathology.
• Conduct experiments following research protocols, maintaining accurate records, and analyzing data for statistical insights.
• Manage laboratory operations, troubleshoot equipment issues, and oversee inventory and cell cultures.
• Serve as a knowledgeable resource within the department, fostering teamwork and providing excellent customer service to stakeholders.

• Proficient in creating analysis datasets and generating Tables, Listings, and Figures from raw data, ensuring accuracy and compliance with study protocols.
• Demonstrated comprehension of study design and related documents such as the Report and Analysis Plan (RAP) and Protocol.
• Experienced in respiratory clinical data analysis across various domains including Adverse Events, Lab, Vital Signs, ECG, Concomitant Medications, and Subject Disposition.
• Skilled in handling parallel, blinded, unblinded, and device studies, ensuring adherence to study protocols and regulatory requirements.
• Developed SAS Macros to streamline processing and standardize code, enhancing efficiency in data analysis.
• Actively involved in maintaining and updating the Web Forum, contributing to the enhancement of the website's functionality.
• Recognized for innovation and collaboration, winning the in-house competition 'Innovation Growth League' for developing a drug dispensing device controlled by a Telegram bot, and contributing to AI processing for its development and training.
• Received a Bronze recognition award for significant contributions to a high-priority study, showcasing dedication and excellence in work performance.