Summary
Overview
Work History
Education
Skills
Websites
Timeline
Generic

MITALI GUPTA

Medford,MA

Summary

Skilled and Dedicated researcher enthusiastic about supporting advancements in research with 3 years of industry experience. Organized, independent and successful at managing multiple priorities with a positive attitude. Willingness to take on added responsibilities to meet team goals.


Overview

3
3
years of professional experience

Work History

Research Associate (Biologist IV)

AbbVie
01.2022 - Current
  • Mastered standard methods of in vitro cell culture using cell lines, primary human cells and cancerous cells.
  • Responsible for Target identification and validation in multiple projects in fibrosis.
  • Performed RNA and protein analysis using qRT-PCR, immunoassays such as Western blot, Jess/ Wes, ELISA, alphaLISA, HTRF in lung and skin fibroblasts for internal target genes/proteins for idiopathic pulmonary fibrosis and systemic sclerosis.
  • Worked extensively on characterizing and studying the gene knockdown efficiency with siRNA.
  • Tested siRNA-mediated gene silencing to assess potential fibrosis target genes in human primary fibroblasts.
  • Evaluated the effects of inhibitors of internal fibrosis targets on cell viability and fibrotic responses in primary fibroblasts by CellTiter-Glo and ELISA analysis.
  • Provided cross-functional support and collaborated with other teams across multiple fibrotic diseases including IPF and SSc.
  • Managed multiple projects simultaneously, ensuring timely completion and high-quality deliverables.
  • Independently designed and executed experiments, analyzed data, and presented findings to a multi-disciplinary team of scientists on a weekly basis.
  • Maintained a safe and efficient laboratory environment by adhering to strict safety protocols and guidelines.
  • Maintained detailed records of project data and materials, updating information when changes arose.

Co-Op Student

AbbVie
05.2021 - 11.2021
  • Provided support in Assay development for early IBD and dermatology target identification and validation.
  • Performed assays on polarized epithelial cell lines (T84) to support early IBD targets. Used target specific CRISPR/Cas9 KO and shRNA KD to monitor the regulatory mechanisms on barrier function and formation. Applied TEER (transepithelial electrical resistance) assay and fluorescent dye permeability measurements to monitor barrier integrity.
  • Cultured and maintained lab stock of colon organoids from human and cynomolgus macaques and assessed drug-induced transcriptional responses in the organoid cultures by qRT-PCR
  • Established techniques for the differentiation and culture of mouse bone marrow-derived mast cells in dermatology projects. Evaluated the purity of mast cells by performing FACs.
  • Gained experience with mouse handling and got trainings for drug administration using IV, IP, SC.

Research Volunteer

Ralph H. Loring Lab
11.2020 - 08.2021
  • Gained hands on experience with mammalian cell culture and aseptic techniques.
  • Supported research on how RIC-3 and other chaperones facilitate α7 nicotinic receptor folding and assembly by performing transfection and binding assays.

Teaching Assistant

Northeastern University
01.2021 - 05.2021
  • Helped students by answering questions related to curriculums, assessments, and projects.
  • Helped with grading assignments and tests, providing constructive feedback to students based on results.
  • Handled class records for attendance, assignment grades and course participation scores.

Notetaker

Disability Resource Center, Northeastern University
09.2020 - 05.2021
  • Summarized the contents of lectures in courses, including Protein Chemistry, Molecular Biology, Drug Product Processes, to help the disabled students for learning.

Education

Master of Science - Biotechnology

Northeastern University
Boston, MA
12.2021

Bachelor of Science - Zoology

Delhi University
Delhi, India
04.2019

Skills

  • Lab Techniques: Cell Culture , Organoid culture, Polarized cell cultures on Transwell insert, qRT-PCR (384 well & 96 well format), Western Blot, ELISA, AlphaLISA, HTRF assay, multicolor FACS, JESS immunoblot, BCA Protein Assay, Transfection, Binding Assay, Gel Electrophoresis, Cell viability assay, Pipetting, Cell counting
  • Lab Equipment: Biosafety Cabinet, ProteinSimple’s Jess, QuantStudio 7 Pro Real-Time PCR System, NanoDrop One/OneC Microvolume UV-Vis Spectrophotometer, Biorad Chemidoc MP, Spectramax ID3, Biorad C100 thermal cycler, Perkin Elmer microbeta, Autoradiogram, Survey meter, Centrifuge, Hemocytometer, Fortessa, Vi-cell counter
  • Lab Safety & Management: General safety in BL2; general lab inventory management; operation of flow hoods; handling, storage & disposal of radioactive and infectious materials
  • Animal work: Euthanizing of mouse, Bone marrow extraction, Sub-cutaneous and Intra-peritoneal injections
  • Softwares: Graphpad Prism, Flowjo, Imagelab, Design and Analysis 2 (QuantStudio 6 and 7 Pro Real-Time PCR System Software), Biorad CFX, Compass, Unicorn 7, MS Excel, MS Word, MS PowerPoint
  • Leadership and Management skills: Successful completion of the teaching assistant in a course in the master's program, helped students familiarize themselves with the laboratory space and teaching lab work during their internship

Websites

Timeline

Research Associate (Biologist IV)

AbbVie
01.2022 - Current

Co-Op Student

AbbVie
05.2021 - 11.2021

Teaching Assistant

Northeastern University
01.2021 - 05.2021

Research Volunteer

Ralph H. Loring Lab
11.2020 - 08.2021

Notetaker

Disability Resource Center, Northeastern University
09.2020 - 05.2021

Master of Science - Biotechnology

Northeastern University

Bachelor of Science - Zoology

Delhi University

Similar Profiles

CREATE PROFILE
MITALI GUPTA