MOHAMED HASSAN

• Automated Chemistry & Immunoassay: Managed daily operations on the AU480 and Access 2, performing routine metabolic panels and complex thyroid/cardiac marker assays with high turnaround efficiency.

• Hematology & Morphology: Conducted complete blood counts (CBC) and white cell differentials using Sysmex technology; validated results by correlating automated data with peripheral blood smear morphology.

• Protein Analysis: Performed Western Blot assays for [mention purpose, e.g., confirmatory infectious disease testing or research], ensuring precise protein separation and antibody binding.

• Quality Assurance: Monitored instrument performance using internal controls and participated in proficiency testing to ensure compliance with CAP/CLIA standards.

• Conducted laboratory tests to analyze samples for medical diagnoses.
• Operated and maintained laboratory equipment and instruments for accurate results.
• Collaborated with healthcare professionals to discuss test findings and implications.

TECHNICAL EXPERTISE & INSTRUMENTATION

Molecular & Genomic Platforms (NGS & PCR)

* Next-Generation Sequencing (NGS): Expert operation and loading of Illumina MiSeq and NextSeq systems

for routine and STAT testing.

* Library Preparation: Skilled in indexing, cleaning, and pooling samples for sequencing.

* Specialized PCR & Mutation Analysis: Performance of complex assays including FLT3 (ITD & TKD) and

CTL.

* Real-Time & RT-PCR: Proficient in Real-Time PCR for Human Leukocyte Antigen (HLA) analysis and RT-

PCR sample processing.

* Rapid Molecular Diagnostics: Utilization of the Solana Molecular Platform and Luminex xMAP Technology

for multiplex pathogen detection.* Quantification: Precise measurement of nucleic acid concentrations using the Qubit Fluorometer and

Spectrophotometry.

Biomarker & Specialized Clinical Testing

* Biomarker Testing: Detecting specific molecules, including proteins and genes, to indicate disease etiology

and pathology.

* Cancer Genomics: Dedicated experience within specialized Cancer Genomics departments assisting with

disease etiology determination and pathology.

* Advanced Diagnostics: Experienced in Nucleic Acid Testing (NAT), Serology, Immunofluorescence

microscopy, and minor Blood Banking.

* Pathology Consultation: Consulted with staff pathologists to complete diagnoses for abnormal cells.

Nucleic Acid Processing & Extraction

* Automated Systems: Advanced use of the QIAsymphony for nucleic acid extraction and automated

normalization.

* Manual Techniques: Expert in manual DNA/RNA isolation and manual normalization.

* Sample Management: Proficient in accessioning, sample preparation, and maintaining strict environmental

monitoring systems.

Lab Operations, Quality & Leadership

* Quality Systems: Execution of QC/QA protocols, proficiency testing, and recording control values to ensure

result reliability.

* Documentation & Compliance: Assisted in creating and revising SOPs, policies, and procedures while

complying with federal and state regulations.

* Training & Mentorship: Demonstrated and trained new laboratory personnel on testing procedures and

equipment.

* Research & Validation: Performed complex procedures to provide data for clinical research and assisted in

the validation of new assays.

* Resource Management: Managed inventory, supplies, and the preparation of testing reagents.

* Equipment Maintenance: Properly disinfected work areas and maintained laboratory instrumentation required

for research and process control.

Professional Experience

Chemistry Laboratory Technologist | MetPath Laboratories

Technical Expertise & Key Responsibilities:

* Clinical Chemistry & Immunoassay (Core Speciality):

* Expertly operated and maintained the Beckman Coulter AU480 analyzer, ensuring high-throughput processing of routine and specialized biochemistry profiles.

* Mastered the Beckman Coulter Access 2 Immunoassay system for precise testing of hormones, cardiac markers, and tumor markers.

* Managed the fully automated ThunderBolt® Analyzer, streamlining ELISA and CLIA workflows with a focus on accuracy and rapid turnaround time (TAT).

* Molecular Virology & Specialized Testing:

* Performed advanced viral diagnostics using Luminex xMAP Technology, specializing in multiplex pathogen detection.

* Utilized the Solana Molecular Platform for rapid nucleic acid amplification, ensuring high sensitivity in virology results.

* Hematology:

* Conducted automated CBC and differential analysis using the Sysmex XN-330, including result validation and monitoring of hematological flags.

* Quality Systems & Operations:

* Executed daily/weekly preventive maintenance and calibration for all platforms (AU480, Access 2, Luminex, etc.).

* Ensured 100% compliance with Internal Quality Control (IQC) and External Quality Assurance (EQA) standards to maintain lab accreditation.

Technical Skills Summary

* Chemistry & Immunoassay: Beckman Coulter AU480, Access 2, ThunderBolt (ELISA/CLIA).

* Molecular & Virology: Luminex (Multiplexing), Solana (Molecular Diagnostics).

* Hematology: Sysmex XN-330.

* Core Competencies: Clinical Chemistry, Immunoassay Validation, Molecular Virology, Equipment Troubleshooting.

• Maintained quality control policies and documented activities such as reagent validations.
• Uphold OSHA and other safety standards to prevent harm to personnel or equipment.
• Completed regular inventories of reagents and supplies.
• Maintained strict quality assurance standard to keep laboratory results accurate.

• Clearly recorded all patient data, including history, exams performed, and past and current medications in corresponding charts.
• Recommended medicines, wrote prescriptions, and observed patient progress.
• Recommended and managed treatment, medication, therapy, and vaccinations to prevent or treat diseases, injuries, and illnesses.

• Conducting the clinical trial according to Good Clinical Practice (GCP).
• Ensure adherence to protocol.
• Conducting study feasibility assessments.
• Onsite initiation visits, monitoring visits and close-out visits.
• Worked within three observational clinical trials in Hypertension, Acute coronary syndrome, and Chronic obstructive pulmonary disease.