Mohammad Rahman
Walpole,MA
Summary
Highly motivated and skillful automation specialist with a proven track record of delivering quality results in Life Sciences. Strong ability to multitask and prioritize workloads with minimal supervision. Looking for a position that combines biology and automation skills with a collaborative and high performing team.
Overview
6
6
years of professional experience
Work History
Automation Specialist
Exact Sciences
05.2023 - 03.2024
- Setup and PM'd Hamilton STAR & STARlet instrumentation via service macro's to quarter Hamilton factory specifications
- Configured Hamilton STAR & STARlet instrumentation to support NGS workflows according to R&D's requirements including sample tracking and data integration
- Created custom liquid classes utilizing Liquid Verification Kit (LVK) for Pico-green fluorescent probes, Proteinase K, and blood buffy coat
- Integrated and assessed small devices, including Inheco Plate Heater/Cooler (CPAC) and On-Deck Thermocyclers (ODTC), Hamilton Heater Shakers (HHS) and Media Fill Modules
- Utilized GitLab for automation project development, from code management with version control through system software deployment via GitBash.
- Provided rapid response to lab automation support requests, initiating troubleshooting and triaging system issues to Process Engineering, Metrology, and IT
- Conducted COGS experiments to further advance automation process by implementing different consumables to reduce reagent dead volumes and increase throughput.
- Developed and deployed a Hamilton method to monitor the accuracy and precision of the 96 channel head and 8 channel pipettes via the Artel Multichannel Verification System.
Senior Research Associate
Exact Sciences
07.2022 - 03.2024
- Lead development for the Pico-Green Fluorescence quantification method on the Hamilton, including creating dilution curves for both post extraction and post Library Construction quantification using the FLOUstar Omega Analysis software.
- Achieved project objectives by producing a Hamilton Venus gDNA extraction method that resulted in a two-fold increase in gDNA yields.
- Conducted experiments ranging from user enzyme titration in library construction to optimizing fragment lengths for target enrichment.
- Designed and executed studies to support usability of reagents, analyze data and provide actionable recommendations to project team.
- Delivered comprehensive training sessions on laboratory techniques and equipment usage, enhancing team members skill sets.
- Tested, edited, and established SOPs concerning gDNA extraction, and Pico-green quantification to support RnD studies.
Molecular Technologist 1 3rd Shift
Foundation Medicine
10.2021 - 06.2022
- Pooled and Clustered libraries to for sequencing on Hi-Seq 4000 and Nova-Seq systems.
- Troubleshot problems with Biomek NXP and provided IQOQ for recently calibrated Thermocycler X50s, also brought serious concerns to The Automation engineering team for problems out of my troubleshooting scope.
- Monitored and diagnosed failing sequencing runs based on Quality Control metrics on Illumina SAV software.
- Over four years of processing human DNA/RNA samples in CLIA/CAP regulated environment.
- Conducted wide range of laboratory tests such as Pico and Oli-Green Quantification and recorded results in regulated batch records.
Research Associate II Contractor
Sanofi
04.2021 - 10.2021
- Extracted and Prepared RNA, WGS, TCR, and Ribodepleted Libraries for sequencing on Nova-Seq and Mi-Seq studies concerning potential therapeutics.
- Utilized Tapestation, q-PCR, and Qubit assays to quantify and normalize DNA and RNA libraries for cancer cell line testing.
- Collaborated with Senior scientist to create a bio-sample management repository.
- Analyzed FASTQ files from Nova-Seqs for Quality Metrics like Pass Filter, Gene Duplications, Gene Error Rates, and Gene Expression
- Gathered, arranged, and corrected research data in Benchling and JMP to create representative graphs and charts highlighting results for presentations
Associate Molecular Technologist Contractor
Ginkgo Biowork
08.2020 - 04.2021
- Performed magnetic bead-based assays to extract viral RNA for Covid-19 diagnostics for sequencing on Nova-Seqs
- Experience in revising SOPS in CLIA regulated environment.
- Performed DNA and RNA quantification assays using q-PCR, Pico-Green and Oli-Green, Qubit, and Tapestation 4200
- Helped management optimize laboratory processes for maximum efficiency and performance.
- Specialized in conducting Concentric branded tests with accuracy and precision.
Lab Operations Assistant
Foundation Medicine
06.2019 - 08.2020
- Supporting personnel responsible for daily maintenance of Agilent BRAVO, Biomek NXP, Spectramax, and HamiltonSTARsystems
- Experience troubleshooting fluidic issues on Hi-Seq 4000 and Nova-Seq DNA Illumina Sequencing Systems
- Worked with reagent QC team to produce and allocate Bait sets for library production.
- Over one year of experience in revising SOPs in CAP, CLIA regulated laboratory.
- Managed and maintained batch records, NCRs, and support documentation.
Sample Management Technician Internship
IDEXX reference Laboratory
05.2018 - 01.2019
- Patient data entry and extensive use of LIMS to track and receive veterinarian samples.
- Logged average of five hundred samples per shift
- Experience in grossing and prepping veterinarian samples for Histopathologists
- Prepared reagents and solutions following standard laboratory formulas and procedures.
Education
Bachelor of Science - Biology
University of Massachusetts Amherst
Amherst, MA12.2018
Skills
- HamiltonSTAR
- Integra VIAFLO 96
- Biomek NXP
- Agilent BRAVO
- Molecular Devices Spectramax
- BMG Labtech FLOUstar Omega
- Agilent Tapestation 4200
- NovaSeq, HiSeq 2500, MiSeq, and NextSeq 1000
SOFTWARE SKILLS
- Venus Hamilton Scripting
- Python Hamilton Integration
- Integra VIALAB
- GitLab, GitHub, and GitBash
- Azure DevOps
- SolidWorks
- JMP and R Statistical Analysis
- Inheco SiLA PMS
- Benchling
- FLOUstar Omega Analysis
- Artel MVS Data Manger
- Illumina SAV
- Microsoft Office Suite
- Confluence
- Jira
Wet Lab Skills
- Assay Development
- DOE and Data presentation
- Reagent QC and stability studies
- Familiarity with NGS workflows
- Statistical analysis
- Optimizing and miniaturizing assays
Hobbies
- Car enthusiast and self-taught mechanic. Currently working on turbo upgrade project.
- Aspiring Linux OS user, only self-instructed at this time. Currently exploring new ways to perform tasks to aid me in the development process.
- Practice Muy Thai and Jujitsu.
Timeline
Automation Specialist
Exact Sciences
05.2023 - 03.2024
Senior Research Associate
Exact Sciences
07.2022 - 03.2024
Molecular Technologist 1 3rd Shift
Foundation Medicine
10.2021 - 06.2022
Research Associate II Contractor
Sanofi
04.2021 - 10.2021
Associate Molecular Technologist Contractor
Ginkgo Biowork
08.2020 - 04.2021
Lab Operations Assistant
Foundation Medicine
06.2019 - 08.2020
Sample Management Technician Internship
IDEXX reference Laboratory
05.2018 - 01.2019
Bachelor of Science - Biology
University of Massachusetts Amherst