Summary
Overview
Work History
Education
Skills
Awards
Lectures
Book Chapter
Publication
Timeline
Generic

Mohammad Shahidullah

Philadelphia,PA

Summary

Analytical method development Expert in biologics characterization, with strong background in HPLC and mass spectrometry for the analysis of antibodies, proteins, and peptides. Besides the above experiences, I have extensive experience studying neuronal ion-channel proteins on cell membranes using a variety of techniques such as patch-clamp and current/voltage-clamp.

Overview

28
28
years of professional experience

Work History

Senior Scientist

Eurofins Lancaster Labs
Malvern, PA
12.2020 - Current
  • The comparability study aims to ensure the quality, safety, and efficacy of a drug product by a changed manufacturing process.
  • This study is being pursued by HPLC technique in a GMP environment.
  • Review the technical documents, and verify the entry data with the source document.
  • Biovia sample management, where drug samples are organized and stressed accordingly (with heat/UV lights), is submitted for further testing to check stability.
  • Ensure GMP compliance for laboratory work, documentation, validation, qualification, and training.

Scientist Lll

Eurofins Lancaster Labs
Malvern, PA
01.2018 - 01.2020
  • Peptide Mapping: Peptide mapping was carried using LC-MS system (in different phases of drugs), analysis data and its documentation using ELN (electronic notebook

Scientist Lll

Eurofins Lancaster Labs
Malvern, PA
09.2016 - 01.2018

Analytical method development: HPLC chromatography and electrophoresis to release samples and characterize drug products under aggressive timelines in a GMP environment, and documentation with ELN.

Research Specialist

University of Pennsylvania School of Medicine
01.2015 - 01.2016
  • Study: cochlear olfactory science using brain slices

Instructor (Research Faculty)

Thomas Jefferson University
01.2010 - 01.2014
  • Study: Synaptic Physiology in ALS disease model

Senior Research Investigator

University of Pennsylvania School of Medicine
01.2006 - 01.2009
  • Study: Ion-channel modulation using in vivo patch-clamp method

Research Associate

University of Pennsylvania School of Medicine
01.2003 - 01.2006
  • Study: M-potassium channel in hippocampal neuron

Post-Doc Training

Thomas Jefferson University
01.1997 - 01.2003
  • Study: Ion-channel gating mechanism using patch-clamp method

Education

Post-Doctoral Training - Neuroscience

Thomas Jefferson University
Philadelphia, PA
05-2003

Ph.D. - Neuroscience

Kanazawa University School of Medicine
Kanazawa, Japan
04-1997

Skills

  • Separation techniques
  • HPLC
  • UPLC
  • Size Exclusion Chromatography
  • Ion Exchange Chromatography
  • Imaged Capillary Isoelectric Focusing
  • LC-MS/MS
  • Analytical Techniques
  • UV-Vis Spectrophotometry
  • SoloVPE
  • Fluorescence Spectroscopy
  • Biochemical Techniques
  • PCR
  • Cloning
  • Protein Expression and Purification
  • SDS-PAGE
  • Data Analysis & Lab Tools
  • Biovia Electronic lab notebook
  • Empower
  • Xcalibur
  • GraphPad prism
  • Origin
  • JUMP
  • Microsoft Office Suite
  • Antibody Characterization
  • Post-translational modifications
  • Characterization of charge variants
  • Disulfide mapping
  • Neuroscientific Assay
  • Patch-clamp recording
  • Current/Voltage-clamp recording
  • Recording on brain slice

Awards

  • Collaborative Professor, Kanazawa University School of Medicine
  • NRSA (National Research Service Award)
  • MONBUSHO, Japanese Government Scholarship

Lectures

Invited Speaker, Innovative Brain Science for Development, Learning, Memory and Autism, Kanazawa, Japan

Book Chapter

Smitha Reddy, Mohammad Shahidullah, Irwin B. Levitan. In Vivo Roles of Ion Channel Regulatory Protein Complexes in Neuronal Physiology and Behavior Structure, Function, and Modulation of Neuronal Voltage-gated Ion Channels Edited by Valentin K. Gribkoff and Leonard K. Kaczmarek; published by John Wiley & Sons, Inc.,

Publication

1) James E.C. Jepson*, Mohammad Shahidullah*, Die Liu, Sylvan le Marchand, Sha Liu, Mark N  Wu, Matthew Dalva, Irwin Levitan, and Kyunghee Koh: Regulation of synaptic development and function by the Drosophila PDZ protein DYSCHRONIC

Development 141(23), 4548-57, 2014  [*First authorship shared]

2) Mohammad Shahidullah, Sylvain LeMarchand, Hong Fei, Jiaming Zhang, Udai Bhan Pandey, Matthew Dalva, Piera Pasinelli and Irwin B. Levitan: Defects in synapse structure and function precede motor neuron degeneration in Drosophila models of FUS-related ALS

Journal of Neuroscience  33(50), 19590-8, 2013

3) Amanda Crocker, Mohammad Shahidullah, Irwin B. Levitan and Amita Sehgal: Identification of a neural circuit that underlies the effects of octopamine on sleep:wake behaviour

Neuron Vol. 65(5): 670-81, 2010

4)  Mohammad Shahidullah, Smitha Reddy, Hong Fei and Irwin B. Levitan: In vivo role of a potassium channel-binding protein in regulating neuronal excitability

Journal of Neuroscience Vol. 29(42): 13328-3, 2009

5) Mohammad Shahidullah, Lindsey Ciali Santarelli, Hua Wen, and Irwin B. Levitan: Expression of a Calmodulin-binding KCNQ2 potassium channel fragment modulates neuronal M-current and membrane excitability

Proc. Natl. Acad. Sci USA Vol. 102(45):16454-16459, 2005

6)  Mohammad Shahidullah, Thanawath Harris, Markus W. Germann and Manuel Covarrubias: Molecular Features of an Alcohol Binding Site in a Neuronal Potassium Channel.

Biochemistry Vol. 42, 11243-11252, 2003

7) Mohammad Shahidullah and Manuel Covarrubias: The link between ion permeation and inactivation gating of Kv4 Potassium channels.

Biophysical Journal; Vol. 84, No 2: 928-41, 2003

8) Mohammad Shahidullah, Naoto Hoshi, Shigeru Yokoyama and Haruhiro Higashida: Microheterogeneity in heteromultimeric assemblies formed by Shaker (Kv1) and Shaw (Kv3) subfamilies of voltage-gated K+ channels.

Proc. R. Soc. Lond. B 261, 309-317; 1995

9) Mohammad Shahidullah, Naoto Hoshi, Shigeru Yokoyama, Tetsuro Kawamura, Haruhiro Higashida: Slow inactivation conserved in heteromultimeric voltage-dependent K+ channels between Shaker (Kv1) and Shaw(Kv3) subfamilies.

FEBS Lett. 371, 307-310; 1995

Timeline

Senior Scientist

Eurofins Lancaster Labs
12.2020 - Current

Scientist Lll

Eurofins Lancaster Labs
01.2018 - 01.2020

Scientist Lll

Eurofins Lancaster Labs
09.2016 - 01.2018

Research Specialist

University of Pennsylvania School of Medicine
01.2015 - 01.2016

Instructor (Research Faculty)

Thomas Jefferson University
01.2010 - 01.2014

Senior Research Investigator

University of Pennsylvania School of Medicine
01.2006 - 01.2009

Research Associate

University of Pennsylvania School of Medicine
01.2003 - 01.2006

Post-Doc Training

Thomas Jefferson University
01.1997 - 01.2003

Post-Doctoral Training - Neuroscience

Thomas Jefferson University

Ph.D. - Neuroscience

Kanazawa University School of Medicine
Mohammad Shahidullah