Mohd Farhan Siddiqui

Biomedical Engineer with 5 yrs of postdoctoral experience in technology and method development to study human-specific biology and diseases.

Productive researcher with proven communication, entrepreneurial and management skills

Reviewer of scientific literature in Biochimica et Biophysica Acta (BBA), and International Journal of Biological Macromolecules

Recipient of highly competitive Scholarship in India for pursuing Master's Graduate Aptitude Test in Engineering (GATE)

Published fourteen articles with >550 citations, Developed patentable technologies and methods, and Filed 5 patens

Biochemistry/Molecular Biology RNA & DNA extraction, RNA purification, cDNA synthesis, quantitative RT-PCR, Gel Electrophoresis, Western Blot, ELISA, Plasmid preps and plasmid DNA extraction, PCR, Absorbance/Fluorescence/Luminescence cell viability/metabolic/differentiation assays, immunohistochemistry, cryopreservation, Cloning, antibody staining, NGS, library preparation, RNA-seq, ScRNA-Seq, CRISPR/Cas9, multiplex assay (luminex)

Microbiological skills Isolation and culture of bacteria, Acid-fast Gram staining, DNA, RNA, protein isolation, MTT assay, DOPA assay, DCFDA assay, Disk diffusion, AlamarBlue assay

In Vitro cultures of mammalian cells (human), isolation of immune cells cells from liver (human), primary cell line; (muscle, liver, adipocytes, PBMCs, pancreas) bacterial culture, electroporation, transfection in mouse cell lines, generating knockout cell lines using gene editing

Nanotechnology techniques Synthesis of nanoparticles/nanocluster, surface characterization, bioconjugation, colorimetric, and fluorescence properties characterization nanoparticle antibody/aptamer/peptide-based assay, and EDC-NHS conjugation chemistry

Imaging and spectroscopic techniques Confocal imaging, 3D thunder live imager, Bright field and fluorescence microscopy, DLS, Zeta potential, AFM, FE-SEM, TEM, UV-vis spectroscopy, Microplate reader

Software MS Office (Word, Excel, Powerpoint), Adobe Photoshop, ImageJ, AutoCAD, SolidWorks, COMSOL Multiphysics (CFD), MS Office, Origin, ImageJ, ChemBioDraw, Adobe Photoshop, RGB-CMYK image processing

1. Siddiqui Mohd Farhan, Uslu Merve and Trapecar Martin. "Development of multiorgan microphysiological system to study the interaction between Gut-Liver-Pancreas-Muscle-Adipocytes for systemic metabolism (2024) Submitted

2. Uslu Merve, Siddiqui Mohd Farhan, Uslu Merve and Trapecar Martin. "Deciphering the role of mucosa-associated invariant (MAIT) T cells in an engineered model of the gut-liver. (2024) Submitted

3. Siddiqui, Mohd Farhan, and Moon-Moo Kim "CRISPR/Cas9 System Mediated SIRT7 Gene Knockout Promotes Melanogenesis by MITF via MAPKS and BMP Activation in Melanoma Cells " Applied Biochemistry and Microbiology (2023)

4. Siddiqui, Mohd Farhan, and Moon-Moo Kim “SIRT7 gene knockout using CRISPR/Cas9 system enhances melanin production in the melanoma cells” Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease 1867 (11), 166219 (2021)

5. Siddiqui, Mohd Farhan, Sojeong Jeon, and Moon-Moo Kim. "Rapid and sensitive detection of melanin using glutathione conjugated gold nanocluster-based fluorescence quenching assay."Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 247 (2021).

6. Siddiqui, Mohd Farhan, Sojeong Jeon, and Moon-Moo Kim. "Monitoring of whitening agent for skin analysis using tyrosinase gold nanoparticle-based colorimetric assay " Asia‐Pacific Journal of Chemical Engineering (2021).

7. Siddiqui, Mohd Farhan, Zeeshan Ahmad Khan, and Seungkyung Park. "Detection of C-Reactive Protein Using Histag-HRP Functionalized Nanoconjugate with Signal Amplified Immunoassay." Nanomaterials 10.6 (2020): 1240.

8. Siddiqui, Mohd Farhan, Zeeshan Ahmad Khan, and Seungkyung Park. "SPE-based soil processing and aptasensor integrated detection system for rapid on-site screening of arsenic contamination in soil." Ecotoxicology and Environmental Safety 196 (2020): 110559.

9. Hanif, Zahid, Siddiqui, Mohd Farhan, and Sung Jea Park. "Hierarchical growth of nickel oxyhydroxide on bacterial cellulose hydrogel: role of water channels in hydrogel to form a hierarchical structure." Asia‐Pacific Journal of Chemical Engineering 15.2 (2020): e2415.

10. Hanif, Zahid, Siddiqui, Mohd Farhan and Sung Jea Park. "Tannic acid-mediated rapid layer-by-layer deposited non-leaching silver nanoparticles hybridized cellulose membranes for point-of-use water disinfection." Carbohydrate Polymers 231 (2020): 115746.

11. Hanif, Zahid, Siddiqui, Mohd Farhan and Sung Jea Park. "Polypyrrole-based Conducting and Antibacterial Hybrid Cellulose Membranes: A Study on the Effect of UV Exposure on the Conductivity and Formation of Silver Nanoparticles." Sensors and Materials 31.6 (2019): 1927-1938.

12. Siddiqui, Mohd Farhan, Khan, Zeeshan A., and Seungkyung Park. "Progress in antibiotic susceptibility tests: a comparative review with special emphasis on microfluidic methods." Biotechnology Letters 41.2 (2019): 221-230.

13. Siddiqui, Mohd Farhan, Khan, Zeeshan A., and Seungkyung Park. "Current and emerging methods of antibiotic susceptibility testing." Diagnostics 9.2 (2019): 49.

14. Siddiqui, Mohd Farhan, et al. "Miniaturized sample preparation and rapid detection of arsenite in contaminated soil using a smartphone." Sensors 18.3 (2018): 777.

15. Kakoti, Ankana, Siddiqui, Mohd Farhan, and Pranab Goswami. "A low-cost design and fabrication method for developing a leak-proof paper-based microfluidic device with customized test zone." Biomicrofluidics 9.2 (2015): 026502.

16. Siddiqui Mohd Farhan and Asif Ishfaque. "Microfluidics approaches for detection of heavy metal ions" Korean Society for Molecular and Cellular Biology conference

1. Martin Trapecar*, Mohd Farhan siddiqui (2023). Multiorgan Tissue Interaction Vessel (MOTIVE). D17854. (USA)

2. Seung Kyung Park*, Mohd Farhan Siddiqui, Zeeshan Ahmad Khan (2019) Bio-probe for detecting target protein, manufacturing method thereof, analysing apparatus having the same and analysing method thereof. Application no. 10-2019-0012873 (South Korea)

3. Seung Kyung Park*, Zeeshan Ahmad Khan, Mohd Farhan Siddiqui (2019) The label-free impedimetric measurement method. Application no. 10-2019-0012052 (South Korea)

4. Pranab Goswami*, Ankana Kakoti, Mohd Farhan Siddiqui (2015). Paper-Based Microfluidic Analytical Device with Prefabricated Customized Test Zone. Application no. 1365/KOL/2014 (India)

5. Pranab Goswami*, Ankana Kakoti, Mohd Farhan Siddiqui (2014). Leak Proof Paper-Based Analytical Microfluidic Device and Process for its Preparation. Application no. 1126/KOL/2014 (India)

Jing Liang                                                                                                        

Research Specialist

Johns Hopkins University School of Medicine (USA)

Department of Biomedical Engineering, USA

jliang62@jh.edu

Martin Trapecar

Assist. Prof. of Medicine and Biomedical Engineering

Johns Hopkins University School of Medicine

Laboratory of Human Biomimetics, USA

+1 (727) 767-8935

mtrapec1@jhmi.edu

Vertica Agnihotri

Postdoctoral Associate

City of Hope

Department of Medicine, USA

vagnihotri@coh.org