Monica (Jushchyshyn) Chu

Extensive knowledge of LC-MS techniques including HRMS, QQQ, SWATH, IDA and EAD methods to analyze small molecules, PROTACs and proteins. Fluent in in vitro DMPK techniques including CYP DI/TDI, protein binding, metabolic stability, reaction phenotyping and SSID. Experienced with managing CRO activities. Hands on involvement with troubleshooting, maintaining, and repairing various laboratory instrumentation.

Qualitative and quantitative LC-MS/MS (Sciex 4000 through 6500 and QTOF 7600, ThermoQuest LC-Q, Deca, TSQ), HPLC (Waters, Agilent, Shimadzu), spectrophotometry, fluorophotometry, electrochemistry, P450 kinetics, metabolite identification, solid phase extraction, Fenton chemistry, SDS-PAGE, Western blot analysis, immunoprecipitation of proteins, proteolytic digestion of proteins, protein expression and purification, protein preparation from animal tissue. Liquid handling platforms such as Tecan, Integra Assist Plus, Echo. Fluent with Sciex OS and Analyst software (Sciex) and Microsoft Office. Proficient with Oniro Massmeta Site.

• Pharmacological Sciences Training Program Fellowship recipient, 1998-2001.
• Thomas Baum Travel Award, February 2001.
• 62nd Intercollegiate Student Chemists Conference, 1st Place, Physical Chemistry, 1998.
• National Science Foundation Research Experience for Undergraduates Fellowship, 1997.
• Chemistry Merit Scholarship, Ursinus College, 1994-1998.

• Jushchyshyn, M.I.; Kent, U.M.; and Hollenberg, P.F. (2001) Electrospray liquid chromatography-mass spectrometry analysis of phencyclidine-inactivated human P450 2B6 and identification of a phencyclidine-adducted peptide. 20th Annual Graduate Student Symposium in the Pharmacological Sciences and Bio-related Chemistry, Ann Arbor, MI.
• Hollenberg, P.F.; Jushchyshyn, M.I.; Chun, J.; Sayre, L.M.; and Kent, U.M. (2000) Metabolic inactivation of rat and human cytochromes P450 by phencyclidine. 219th American Chemical Society Meeting, San Francisco, CA.
• Jushchyshyn, M.I.; Kent, U.M.; and Hollenberg, P.F. (1999) Mechanism-based inactivation of human P450 2B6 by phencyclidine. 28th Annual Pharmacology Research Colloquium, East Lansing, MI.

• Lin HL, Zhang H, Jushchyshyn M, Hollenberg PF. (2010) Covalent modification of Thr302 in cytochrome P450 2B1 by the mechanism-based inactivator 4-tert-butylphenylacetylene. Journal of Pharmacology and Experimental Therapeutics 333: 663-669.
• Jushchyshyn, M.I.; Wahlstrom, J.L.; Hollenberg, P.F.; Wienkers, L.C. (2006) Mechanism of Inactivation of Cytochrome P450 2B6 by Phencyclidine. Drug Metabolism and Disposition 34: 1523-1529.
• Shebley, M.; Jushchyshyn, M.I.; Hollenberg, P.F. (2006) Selective Pathways for the Metabolism of Phencyclidine by Cytochrome P450 2B Enzymes: Identification of Electrophilic Metabolites, Glutathione and N-Acetylcysteine Adducts. Drug Metabolism and Disposition 34: 375-383.
• Jushchyshyn, M.I.; Hutzler, J.M.; Schrag, M.L.; Wienkers, L.C. (2005) Catalytic Turnover of Pyrene by CYP3A4: Evidence that Cytochrome b5 Directly Induces Positive Cooperativity. Archives of Biochemistry and Biophysics 438: 21-28.
• Jushchyshyn, M.I.; Kent, U.M.; and Hollenberg, P.F. (2003) The Mechanism-Based Inactivation of Human Cytochrome P450 2B6 by Phencyclidine and Investigations of the Identity of the Covalently Modified Peptide. Drug Metabolism and Disposition 31: 46-52.
• Kent, U.M.; Jushchyshyn, M.I.; and Hollenberg, P.F. (2001) Mechanism-based Inactivators as Probes of Cytochrome P450 Structure and Function. (Review) Current Drug Metabolism 2: 215-243.

• Handler of a therapy certified dog, Chipper. We visit libraries, schools, and doctors’ offices.
• Three years on the planning committee for the Bear2Bear emergency student fund at Ursinus College. Helped with planning all aspects of this annual fundraising event including obtaining sponsorships, program ads and auction items as well as bolstering faculty involvement.
• Currently serving on the Major Gifts Committee at Ursinus College. Involved with communications to Ursinus community members to raise funds for annual giving.
• Science Fair judge: Judged numerous student science fairs.