Moushumi Hossain-Gjukis
Portage,USA
Summary
Professional ready to contribute to organizational success with strong operational and administrative skills. History of delivering impactful support and fostering team collaboration to meet dynamic needs. Led and conducted investigations and performed root cause analyses using DMAIC and PHP tools. Expert in analyzing and interpreting experimental data, results, and methodology, as well as documenting and communicating results in laboratory notebooks, technical reports, and oral presentations. Reliable and adaptable, with focus on effective communication and problem-solving.
Overview
20
20
years of professional experience
Work History
Bioanalytical Testing Lab Staff Colleague
Pfizer Global Supply
04.2022 - 04.2024
- Company Overview: Quality Operations – Kalamazoo, MI
- Led complex analytical testing initiatives to bolster quality programs within the division, collaborating with cross-functional teams to meet project deliverables; conducted investigations and root cause analyses (CAPA) using DMAIC and PHP tools
- Proven subject matter expertise (SME) on various bioanalytical assays ranging from histopathology to capillary gel electrophoresis (mRNA and Protein) by data analysis, troubleshooting, providing guidance and problem-solving technical issues to successfully launch product into the market
- Orchestrated the integration of R&D and CRO technologies for on-site quality control, delivering a $500K cost reduction annually
- Managed equipment qualification, validation, and compliance ensuring PQS alignment and successful co-validation processes
- Collaborated with site cross-functional areas and vendors to support project goals, objectives and timelines, which included understanding needs in establishing a new technology, method transfers, hands on feasibility and validation assays, itemizing budget needs and projection of timelines
- Oversaw and trained lab analysts on cutting-edge technology to build company’s technology footprint
- Supported technology (mRNA and protein analytical testing) tour presentations and regulatory audits
- Authored, updated and reviewed technical documentation associated with Good Manufacturing Practices {also cGMP}, method validation, investigation of change controls and technical reports
- Quality Operations – Kalamazoo, MI
Group Leader/Supervisor
Eurofins Lancaster Laboratories
08.2018 - 04.2022
- Company Overview: Kalamazoo, MI
- Led, mentored, and developed up to sixteen scientists with diverse backgrounds, providing new employees mid-year and annual reviews
- Created hiring initiatives resulting in the recruitment of the best scientists across cross-functional areas
- Ensured quality and efficiency metrics were set and met for each direct report via weekly updates with specifics using a spreadsheet to capture work completed, allowing identification of areas for cross-training
- Professional Scientific Staffing - Kalamazoo, MI
Senior Scientist
Eurofins Lancaster Laboratories
10.2012 - 04.2022
- Company Overview: Kalamazoo, MI
- Performed literature research on basic biology of drug targets, molecular pathways, and relevant disease states
- Designed, implemented, and delivered drug discovery and development assays and for multiple research projects
- Developed and established cell-based assays for phenotypic screening, functional pathway analysis and potency assays with mammalian cell lines and primary cells for protein and small molecule therapeutics
- Led and established in vivo and cell-based model assessment, initial drug screening funnel development using RT-qPCR
- Developed and established in situ hybridization in-house within six months, producing high quality results used for upstream data packages within 10 months
- Created and proposed Epitope mapping plan using hydrogen exchange and mass spectrometry to outline drug-target interaction
- The method was adopted by the team and is used regularly
- Designed, developed, and optimized assays for antibody discovery, library generation initiatives and cloning using various PCR approaches, identification, characterization, and screening for therapeutic and diagnostics research
- Expressed recombinant proteins in bacterial and HEK mammalian cell lines
- Kalamazoo, MI
Graduate Assistant, Chemistry and Biochemistry
University of Notre Dame
01.2004 - 01.2012
- Company Overview: South Bend, IN
- Oversaw design of experiments and utilized a variety of laboratory equipment
- Performed research data collection and documentation
- Researched scientific literature and summarized relevant findings
- Directed, designed, and solved rare protein crystal structures of two antigen-bound t-cell receptors (TCR) and of the unbound TCR structure
- Developed a high affinity TCR for potential use in melanoma therapies
- Mentored and managed two undergraduate students and two graduate students
- South Bend, IN
Teaching Assistant, Department of Chemistry and Biochemistry
University of Notre Dame
01.2005 - 01.2007
- Company Overview: South Bend, IN
- Organic Chemistry Lab and Biochemistry Lectures
- Led weekly discussion/question sessions on material covered in class and evaluated/graded examinations
- South Bend, IN
Education
Bachelor of Arts (BA) - Biochemistry and Environmental Science
Olivet College
Olivet, MIDoctor of Philosophy (Ph.D.) - Chemistry and Biochemistry
University of Notre Dame
Notre Dame, INSkills
- Diagnostic & Analytical Technologies
- Project Management
- Group Leader
- Immunology
- Cell Biology
- Statistical Analysis
- Molecular Biology
- Biochemistry & Protein Chemistry
- Good Laboratory Practice (GLP)
- Good Manufacturing Practice (GMP)
- Drug Discovery
- Quality Control
- Method and Technology Transfer
Publishedproteinstructures - Rcsbpdbcodes
- 4EUP
- 4EUQ
- 4EUR
Posterspresentations
- T-cell receptors used in gene therapy of cancer engage and cross-react via distinct peptide-driven mechanisms, Mike and Josie Harper Cancer Research Institute Dedication Ceremony, Notre Dame, IN, 03/01/11
- Molecular description of how different MART-1 antigenic peptides influence T cell receptor, Indiana Clinical and Translational Research Institute (CTSI), Indianapolis, IN, 06/01/11
- A Comparative Study of Different MART-1 Specific TCRs Interacting With Structurally Distinct Ligands, Autumn Immunology Conference (AIC), Chicago, IL, 11/01/09
- The specificity of MART-1 peptide-MHC recognition by a clonally distinct TCR, Autumn Immunology Conference (AIC), Chicago, IL, 11/01/08
- Understanding the specificity of MART-1 peptide-MHC recognition by a clonally distinct TCR, Chemistry, Biology, Biochemistry Interface (CBBI) Program, Notre Dame, IN, 05/01/08
- Expression of the MART-126/27-35 specific TCR JKF6, University of Notre Dame. Biochemistry Departmental Retreat, Notre Dame, IN, 05/01/07
- Regulation of cell function by modified surface chemistries, Center of Fundamental Materials Research Symposium, East Lansing, MI, 05/01/04
Publications
- Computational Design of the Affinity and Specificity of a Therapeutic T Cell Receptor, Pierce B.G; Hellman L.M.; Hossain M.; Singh N.K; Kooi C.W.V.; Weng Z.; Baker B.M., PLOS Computational Biology, 2014, 10, 2, 10.1371/journal.pcbi.1003478
- T-cell Receptor Specificity Maintained by Altered Thermodynamics, Madura F, Rizkallah PJ, Miles KM, Holland CJ, Bulek AM, Fuller A, Schauenburg A.J.A., Miles J.J., Liddy N., Sami M., Li Y., Hossain M., Baker B.M., Jakobsen B.K, Sewell A.K., and Cole D.K., J Biol Chem, 2013, Jun 28, 288, 26, 18766-75
- Evidence for a dynamically driven, allosteric T cell signaling mechanism, Hawse W.F., Champion M.M., Joyce M.V., Hellman L.M., Hossain M., Ryan V., Pierce B.G., Weng, Z., and Baker B.M., The Journal of Immunology, 2012, 188, 12, 5819-23
- TCRs used in Gene Therapy Cross-React with MART-1/Melan-A Tumor Antigens via Distinct Mechanisms, Borbulevych O.Y., Santhanagopalan S.M., Hossain M., and Baker B.M., The Journal of Immunology, 2011, 187, 5, 2453-63
- Loss of T cell antigen recognition arising from changes in peptide and major histocompatibility complex protein flexibility: implications for vaccine design, Insaidoo F.K., Borbulevych, O.Y., Hossain M., Santhanagopalan, S. M., Baxter, T.K., and Baker, B. M., Journal of Biological Chemistry, 2011, 286, 40163-40173
- Regulation of cell function by modified surface chemistries, Porter K., Hossain M., Wang M., Baker G.L., Smith M.R., McCabe L.R., 2006, Molecular Biology Reports, 33, 1, 1-12
- Hepatocyte growth factor (HGF) adsorption kinetics and enhancement of osteoblast differentiation on hydroxyapatite surfaces, Hossain M., Irwin R., Baumann M.J., McCabe L.R., 2005, Biomaterials, 26, 15, 2595-02
Expertise
Mammalian Cell Culture (Cell lines, primary and pluripotent) including co-cultures, Bacterial Cultures, Fluorescence Microscopy, Gene Expression using PCR, RT-PCR, qPCR, RACE PCR, Flowcytometry, Molecular Pharmacology, Mammalian and bacterial protein/antibody engineering, Purification and biophysical characterization, Protein Binding Assays, Protein Electrophoresis, Capillary Gel Electrophoresis (CGE), Western Blot, ELISA, Solid Phase Peptide Synthesis, MALDI-TOF Mass Spectrometry, Chromatography, Protein Crystallization and Structure Determination, Gene Sub-Cloning, Immunohistochemistry (IHC), In Situ Hybridization (ISH), Immunofluorescence Assays (IFA), Immunocytochemistry (ICC), DNA, RNA, and protein extraction and analysis
Timeline
Bioanalytical Testing Lab Staff Colleague
Pfizer Global Supply
04.2022 - 04.2024
Group Leader/Supervisor
Eurofins Lancaster Laboratories
08.2018 - 04.2022
Senior Scientist
Eurofins Lancaster Laboratories
10.2012 - 04.2022
Teaching Assistant, Department of Chemistry and Biochemistry
University of Notre Dame
01.2005 - 01.2007
Graduate Assistant, Chemistry and Biochemistry
University of Notre Dame
01.2004 - 01.2012
Bachelor of Arts (BA) - Biochemistry and Environmental Science
Olivet College
Doctor of Philosophy (Ph.D.) - Chemistry and Biochemistry
University of Notre Dame
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