Nathan Greve
Madison,WI
Summary
Adept at supporting project success and innovation, I leveraged technical troubleshooting and data analysis skills at Promega, significantly contributing to high-priority R&D projects. My expertise in laboratory techniques and collaborative experiment design, honed across roles, underscores my ability to deliver impactful results and support key developments in the biotechnology sector.
Overview
19
19
years of professional experience
Work History
Research Scientist, R&D Flex Team
Promega
05.2022 - 10.2024
- Moved between various different R&D teams to support the development of Promega's most high profile and high priority projects. (e.g. Participated in Method Validation for OncoMate® MSI Dx Analysis System)
- Utilized expertise in assay design, cell culture, luminescent and fluorescent assays, PCR, protein purification, lab automation, and a wide variety of other techniques and tools.
- Collaborated with project partners on experiment design and priority.
- Analyzed experimental results and contributed to data presentation on intra-team meetings, as well as meeting with outside collaborators.
Research Scientist, Nucleic Acid Tech Group
Promega
05.2008 - 05.2022
- Added to Promega's nucleic acid purification portfolio by contributing to the development of the ReliaPrep FFPE gDNA and Total RNA systems.
- Explored several technical avenues to developing a DNA integrity assay, including fluorescent end-labeling, fluorescence polarization of labeled DNA, and ultimately a multiplexed qPCR assay.
- Evaluated Advanced Analytical's Fragment Analyzer (since purchased by Agilent) for possible development into Promega's Spectrum Instrument.
- Used experience with PCR technology to create DNA contamination and nuclease activity QC protocols for several buffers and enzymes manufactured under cGMP conditions.
- Collaborated with QC and Production departments to perform Method Transfer from R&D.
- Developed and launched gene expression assays to monitor differentiation of human and mouse stem cells.
- Developed an RT-qPCR assay to differentiate the source of small drops of bodily fluid. Intended for the forensics market.
Undergraduate Research Assistant
Michael Thomas Lab, UW-Madison
05.2005 - 12.2007
- Assisted a graduate student in the study of a biosynthetic gene cluster in Pseudomonas syringae.
- Generated deletion mutant of Streptomyces coelicolor for use in future metagenomic studies.
- Performed gene function assays.
Education
Bachelor of Science - Biochemistry
University of Wisconsin - Madison
Madison, WI05.2008
Bachelor of Arts - Theology
Immanuel Lutheran College
Eau Claire, WI05.2002
Skills
- Technical Troubleshooting
- Project Support
- Data Analysis
- Experimental Design
- Microsoft Office
- PCR
- Spectrophotometry
- Cell Culture
- Capillary Electrophoresis
- Nucleic Acid Purification
Timeline
Research Scientist, R&D Flex Team
Promega
05.2022 - 10.2024
Research Scientist, Nucleic Acid Tech Group
Promega
05.2008 - 05.2022
Undergraduate Research Assistant
Michael Thomas Lab, UW-Madison
05.2005 - 12.2007
Bachelor of Science - Biochemistry
University of Wisconsin - Madison
Bachelor of Arts - Theology
Immanuel Lutheran College
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