Nathaniel Pontefract

• Conducted functional validation of suspected nodulation-relateing genes in peanuts via cas9 editing.
• Managed bacterial cultures of Agrobacterium and E Coli for plant tissue inoculation.
• Executed molecular biology protocols, including DNA extraction, PCR, and gel electrophoresis.
• Collaborated in troubleshooting experiments and refining bacterial culture protocols to enhance transformation efficiency.
• Delivered data-driven presentations in weekly lab meetings, showcasing research progress and findings.
• Mentored new lab members in molecular biology techniques, including bacterial transformation and gene editing methods.

University Teaching Assistant: PLS 3421C - Hydroponics Systems Course (Summer B)

• Constructed and calibrated hydroponic systems for student experiments on nutrient uptake and plant growth.
• Guided students in maintaining nutrient delivery systems, monitoring sensors, and interpreting real-time data for class projects.
• Developed troubleshooting guides and provided individualized support to students encountering technical issues.

• Filled and performed VIP inspections (certification #32197) on SCUBA cylinders to ensure structural integrity and compliance.
• Built and tested SCUBA gear prior to purchase to verify quality control.
• Completed minor repairs on customer equipment.
• Educated customers on daily weather conditions, spearfishing/lobstering regulations, and local resources such as boat ramps.
• Maintained and organized merchandise.