Nicholas Makogonov

Immunofluorescence

• Designed and executed staining protocols for a variety of animal and patient tissue samples. Managed antibody inventory and purchasing.
• Imaged samples, used ImageJ and QuPath to generate data from slides.
• Used R and MatLab to analyze the data, create graphics for publications.

Genetic Testing and Animal Management

• Optimized protocols for PCR for a variety of primers and tissue samples.
• qPCR
• RNASeq
• Managed all research animals, collected tissue samples, tracked all breeding and the test results for each animal.
• Made major improvements to the computer system used for animal tracking.

• Wrote and executed cutting patterns.
• Routine maintenance on CNC laser machines and associated equipment.
• Maintained a safe working environment by following proper safety protocols and guidelines for CNC laser operation.
• Managed inventory levels of raw materials,