Ph.D. - Microbiology (Virology)
Japan National Institute of Public Health
Tokyo, Japan
Ning Tong
Rochester,NY
Summary
Highly dependable biomedical scientist with a solid academic background and extensive research experience in Neurosciences, Cell/Molecular Biology, CRISPR gene editing, and Biochemistry. Skilled in diverse experiments with strong organizational and communication skills. Demonstrates robust multitasking, problem-solving, and adaptability in challenging environments, excelling both independently and collaboratively. Seeking a senior scientist position in the biomedical industry to apply proven skills and contribute significantly.
Skills
- Experienced in Primary neuronal cell and PBMC isolations, glia-neuron co-culture, Matrigel 3D-culture, CLARITY for brain mapping, single cell cloning.
- Proficient in generating knockout/in cell lines using CRISPR-Cas/lentiviral systems.
- Skilled in Phase contrast/fluorescent microscopy; familiar with confocal and multiphoton microscopy.
- Expertise in small animal handling, surgery, stereotaxic microinjection breeding, back-crossing, genotyping.
- Strong background in RT-PCR, qRT-PCR, DNA/RNA extraction, DNA cloning, RNA FISH/Flow-FISH, RCA, RNA in vitro transcription.
- Proficient in the assays of ATP, MTT TUNEL, ELISA, luciferase, cytokine, EMSA, and Western blot, ICC, IHC, IP, Flow cytometry.
- Software: MS Office, Sigma Plot, Adobe Photoshop, GenBank, Snap Gene, Benchling, FCS Express, Flowjo, GraphPad.
Education
M.D. - Medicine
Tongji Medical University
Wuhan, ChinaWork History
Research Assistant Professor/Founding Lab Manager
Department of Biochemistry, University of Rochester
2017.06 - Current
- Contributed to study of impact of THUMP-D1 on tRNA processing
- Conducted research on CRISPR alternative splicing based on RNA-targeting CRISPR-Cas systems
- Generated CRISPR-Cas-based THUMP-D1 Knockout cell lines
- Developed CRISPR RNA flow cytometry protocol
- Contributed to successful grant fundings, managed lab routine operations, and organized laboratory meetings and journal clubs
- Mentored students, predoctoral trainees, and junior lab members in diverse experimental techniques.
Post-doctoral Research Fellow/Research Assistant Professor
Department of Neurology/Center for Neurotherapeutics Discovery (CND), University of Rochester
1997.06 - 2017.06
- Studied the soluble factors associated with activation of innate immune cells by HIV-1
- Conducted and participated in studies of neuronal toxicities of platelet-activating factor and HIV-1 Tat
- Involved in developing disease models of the central nervous system using confocal and multiphoton microscopy
- Engaged in developing the mouse CLARITY system for studying HIV-1 associated neurocognitive disorders (HAND)
- Contributed to successful grant fundings
- Mentored students, predoctoral trainees, and junior lab members in diverse experimental techniques.
Timeline
Research Assistant Professor/Founding Lab Manager
Department of Biochemistry, University of Rochester
2017.06 - Current
Post-doctoral Research Fellow/Research Assistant Professor
Department of Neurology/Center for Neurotherapeutics Discovery (CND), University of Rochester
1997.06 - 2017.06
Ph.D. - Microbiology (Virology)
Japan National Institute of Public Health
M.D. - Medicine
Tongji Medical University
PUBLICATIONS
- Broly M, Polevoda BV, Awayda KM, Tong N, Lentini J, Besnard T, Deb W, O'Rourke D, Baptista J, Ellard S, Almannai M, Hashem M, Abdulwahab F, Ganesh A, Al-Mamari W, Lim SC, Pais LS, Brown N, Riazuddin S, Bézieau S, Fu D, Isidor B, Cogné B, O'Connell MR. THUMPD1 bi-allelic variants cause loss of tRNA acetylation and a syndromic neurodevelopmental disorder. Am J Hum Genet. 2022 Apr 7;109(4):587-600. PMID: 35196516
- Seth Perry, Justin Barbieri, Ning Tong, Oksana Polesskaya, Santosh Pudasaini, Angela Stout, Rebecca Lu, Michelle Kiebala, Sanjay Maggirwar, and Harris Gelbard. Human immunodeficiency virus-Tat enhances surface levels of the dopamine transporter to increase transporter-specific uptake and Vmax. J. Neurosci. 2010 Oct 20;30(42):14153-64. PMID: 20962236.
- Lu SM, Tong N, Gelbard HA. The phospholipid mediator platelet-activating factor mediates striatal synaptic facilitation. J Neuroimmu. Pharmacol. 2007 Jun;2(2):194-201. 2007 Feb 27. PMID: 18040844.
- Tong, N., Sanchez, J.F., Maggirwar, S.B., Ramirez, S., Dewhurst, S., and H.A. Gelbard. Activation of Glycogen Synthase Kinase 3 beta (GSK-3b) by Platelet Activating Factor Mediates Migration and Cell Death in Cerebellar Granule Neurons. Eur. J. Neurosci. 2001, 13:1913-1922. PMID: 11403684.
- Guo, H., Tong, N., Turner, T., Epstein, L., McDermott, M.P., Kilgannon, P., and H.A. Gelbard. Release of the Neuronal Glycoprotein ICAM-5 in Serum after Hypoxic-Ischemic Injury. Ann. Neurol., 2000, 48:590-602. PMID: 11026442
- Maggirwar, S.B., Ramirez, S., Tong, N., Gelbard, H.A., and S. Dewhurst. Functional interplay between NF-kB and c-Jun integrated by coactivator p300 determines the survival of nerve growth factor-dependent PC12 cells. J. Neurochem., 2000, 74:527-539. PMID: 10646503
- Tong, N., Perry, S., Zhang, Q., James, H.J., Guo, H., Brooks, A., Bal, H., Kinnear, S.A., Fine, S., Epstein, L.G., Dairaghi, D., Schall, T.J., Gendelman, H.E., Dewhurst, S., Sharer, L., and Gelbard, H.A. Neuronal Fractalkine Expression in HIV-1 Encephalitis: Roles for Macrophage Recruitment and Neuroprotection in the CNS. J. Immunol., 2000, 164:1333-1339. PMID: 10640747
- S. B. Maggirwar, N. Tong, S. Ramirez, H.A. Gelbard and S. Duwhurst. HIV-1 Tat Mediated Activation of Glycogen Synthase Kinase-3 (GSK-3) Contributes to Tat-Mediated Neurotoxicity. J Neurochem. 73(2):578-86, 1999. PMID: 10428053
- N. Tong, E. Nobusawa, M. Morishita, S. Nakajima and K. Nakajima. M protein Correlates with the Receptor-binding Specificity of Haemagglutinin Protein of Reassortant Influenza A (H1N1) Virus. J. Gen. Virology, 79:2425-2434, 1998. PMID: 9780048
- N. Tong, K. Nakajima, and S. Nakajima. Identification of the Site for Suppressor Mutations on the Haemagglutinin Molecule to Temperature-sensitive Phenotype of the Influenza Virus. Microbiol. Immunol., 39:687-692, 1995. PMID: 8577282
References
Available upon request
Citizenship
US Citizen.
Languages
English
Full Professional
Chinese (Mandarin)
Native or Bilingual
Japanese
Full Professional
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