Oluwatomisin Atolagbe

Department of Immunology and Microbiology

Center for Cell and Gene Therapy

• Routinely work on the preclinical development of chimeric antigen receptor (CAR) T-cell products for glioblastoma and medulloblastoma treatments
• Goal is to improve CAR T cell therapies for pediatric patients with solid tumors

Department of Immunology

Lab mice techniques

• Assisted the team on projects that investigate and exploit T cell mechanisms in the context of immune checkpoint therapy
• Extracted mice bone marrow and cultured them into bone marrow-derived macrophages using interferon gamma (M1 macrophages) or interleukin-4 (M2 macrophages)
• Handled and restrained mice for purposes including but not limited to tumor implantation, tumor measurement, and treatment
• Dissected and processed mice tissues for experiments such as single-cell RNA sequencing and CyTOF
• Ordered mice, updated, and kept track of laboratory animal inventory

Cell culture

• Used sterile technique to maintain cell lines such as the B16F10 melanoma and MC38 colon cancer lines
• Ordered and restocked tissue culture supplies

Lab maintenance

• Ordered and restocked lab supplies
• Maintained lab equipment
• Ensured proper functioning of lab for daily operations
• Documented standard operating procedures

Program in Bioinformatics/Department of Physiology and Pharmacology

• Goal of thesis project was to identify and validate genes involved in the differential actions of TRPC3 (transient receptor potential canonical 3) in the macrophages
• Performed RNA-sequencing analysis of the transcriptome of macrophage subsets M1 and M2
• Extracted mice bone marrow and cultured them into bone marrow-derived macrophages
• Macrophages were derived from wild type mice or mice with macrophage-specific loss of TRPC3 function

Department of Biochemistry and Molecular Biology

• Tested the expression of Wnt proteins - a set of proteins crucial in embryonic development- in Escherichia coli to produce proteins on a larger scale
• Organized and kept detailed records of data
• Documented standard operating procedures in laboratory notebook

• Worked with a team of researchers to assess levels of free radicals in untreated or OKN-007 treated F98 rat gliomas
• Obtained rodent models' data for research project
• Analyzed and kept record of data

• Prepared media used in laboratory experiments
• Set up and sterilized equipment used in weekly laboratory experiments
• Organized and maintained biological supplies in laboratory
• Inspected laboratory emergency units such as eye-wash and shower stations for proper functioning weekly/bi-weekly