Oumie Secka
New York,NY
Summary
Results-driven Senior Research Technician with expertise in T cell manufacturing, cell culture management, and laboratory documentation. Proven ability to conduct impactful experiments and ensure compliance with GLP and GMP standards.
Overview
6
6
years of professional experience
1
1
Certification
Work History
Senior Research Technician
MEMORIAL SLOAN KETTERING CANCER CENTER
01.2021 - 06.2025
- PBMC separation using the Ficoll method.
- Set up, feed, and passage cells in a contamination-free culture.
- T-cells are modified and armed T cells (CAR-T) with capabilities to better fight cancerous cells in patients.
- Generate viral-specific CTLs (vCTLs) against a single or multiple viruses.
- Use the CliniMACS Prodigy device, which is an automated cell manufacturing system on a closed and scalable platform.
- Run flow cytometry using MACSQuant 10 for cell immunophenotyping.
- Immune cell enrichment using beads.
- Freeze and thaw cells for research and clinical use.
- Conducted laboratory experiments to support cancer research initiatives and studies.
- Analyzed research data and documented findings for scientific publications.
- Maintained laboratory equipment and ensured compliance with safety regulations.
- Managed inventory of supplies and ordered materials for ongoing experiments.
- Maintained lab equipment including calibration of instruments according to manufacturer's specifications.
- Participated in departmental meetings to discuss research progress and share insights.
Senior CMC Operator
MEMORIAL SLOAN KETTERING CANCER CENTER
08.2021 - 12.2022
- Independently follow detailed instructions, as outlined in SOPs for GMP processes.
- Prepare EVB and CMV cytotoxic T cells from liquid nitrogen for patient infusion.
- Perform non-GMP procedures for preclinical research under general instructions, using prior knowledge and experience.
- Make observations of the outcomes or pitfalls of the procedures performed, and communicate them to the supervisor.
- Provide training and oversight to the junior CICT staff, as per the assignment of the CMC Manager.
- Keep records of supplies, reagents, and equipment use.
- Generate detailed documentation (paper-based and electronic) for GMP and non-GMP processing in CICT according to SOP.
Research Technician
RUTGERS UNIVERSITY
Newark, USA
04.2019 - 11.2019
- Company Overview: Rutgers University (New Jersey Medical School, Department of Infectious Diseases)
- Run cell culture and macrophage infection assays.
- Run cell cytotoxicity assays.
- Culture and perform MIC assays of BSL-2 pathogens.
- Works safely in a BSL-3 environment.
- Keeps detailed records of procedures and experiments.
- Maintain the day-to-day running of the laboratory.
- Maintained laboratory equipment and ensured proper functioning and safety standards.
Education
Master's - Immunology and Immunogenetics
UNIVERSITY OF MANCHESTER
Manchester, United Kingdom09.2013
Bachelor's - Microbiology
UNIVERSITY OF MANCHESTER
United Kingdom06-2010
Skills
- T cells Manufacturing
- Cell culture management
- T-cell modification
- Inventory management
- Laboratory documentation
- Experimental protocol development
- Effective communication
- GLP and GMP knowledge
- ELISA and western blot
- Cell culture expertise
- Microbiological techniques
- Conducting experiments
- Training
- Research
- Multitasking
- Time Management
- Problem Solving
- Immunophenotyping
- Laboratory Safety
- Cell Therapy
- Flow Cytometry
Certification
- Clinical laboratory Technologist (Restricted to stem cell process)
- The University of the State of New York Education Department
- Issued: 02/2025
Timeline
Senior CMC Operator
MEMORIAL SLOAN KETTERING CANCER CENTER
08.2021 - 12.2022
Senior Research Technician
MEMORIAL SLOAN KETTERING CANCER CENTER
01.2021 - 06.2025
Research Technician
RUTGERS UNIVERSITY
04.2019 - 11.2019
Master's - Immunology and Immunogenetics
UNIVERSITY OF MANCHESTER
Bachelor's - Microbiology
UNIVERSITY OF MANCHESTER
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