Hi, I’m
Patrick W. Gutschow
Director Of Assay Development
San Diego,CA
Summary
Dedicated scientific professional with a history of meeting company goals utilizing consistent and organized practices. Skilled in working under pressure and adapting to new situations and challenges to best enhance product performance. Detailed-oriented, organized and dependable candidate successful at managing multiple projects with a positive attitude and willing to take on added responsibilities to meet team goals. To seek and maintain a full-time position that offers professional challenges utilizing interpersonal communication, excellent time management and problem-solving skills. Proficient in using independent decision-making skills and sound judgment to positively impact company success.
Overview
18
years of professional experience
5
years of post-secondary education
Work History
Intrinsic LifeSciences, LLCLa Jolla, CA
Director of Assay Development
05.2016 - Current
Job overview
- Managed development efforts to expand product catalog of ELISA kits
- Served as project manager on more than 15 contract research projects including data transfer agreement negotiation, sample management, workflow planning, data integrity, and reporting
- Principle writer including peer-reviewed publications, NIH grant applications (Phase I and Phase II), marketing material, and whitepapers
- Manufacturing and quality control of specialty reagents for kit production
- Oversaw operations and provided corrective feedback to achieve daily and long-term goals
- Worked closely with organizational leadership and board of directors to guide operational strategy
- Drafted and distributed reports to assist board members with critical business decisions
Intrinsic LifeSciences, LLCLa Jolla, CA
Scientist II
09.2011 - 05.2016
Job overview
- Hybridoma antibody production, purification, and ELISA based screening
- Developed 6 novel competitive and direct ELISA assays for clinical and RUO applications
- CAP/CLIA laboratory derived test (LDT) validation principles
- Authored and revised SOPs
- Established manufacturing and quality control protocols for commercial kit reagents
- Conducted experiments in laboratory environment for product development purposes
- Performed complex troubleshooting and assisted in leading initiatives to solve challenging scientific problems with assays
- Simultaneously managed several complex projects, meeting all pertinent milestones
Rancho Tissue Technologies, IncRancho Santa Fe, CA
Research Associate
01.2009 - 05.2011
Job overview
- Combined current literature research with prior colleague data to establish aseptic culture protocols for initiation, multiplication, and rooting of horticulture plants
- Managed media database and weekly media production
Dr. David Traver, UCSDSAN DIEGO, CA
Staff Research Associate
09.2007 - 08.2008
Job overview
- Designed, cloned, and expressed fusion proteins in vitro
- Managed and manipulated cell culture lines
- Screened cDNA library via in situ hybridization for novel markers of hematopoietic lineage
- Independently executed and optimized experimental procedures
- Performed research into study topics to increase knowledge and to provide valuable contributions
- Participated in lab meetings and project presentations
- Recorded and analyzed data to produce reports of results
Dr. David Traver, UCSDSAN DIEGO, CA
Master's Degree Program
07.2005 - 09.2007
Job overview
- Developed recombineering-based strategy to clone large promoter elements used to drive reporter fluorophores in transgenic zebrafish lines
- Analyzed stable transgenic lines with fluorescence microscopy and RT-PCR
- Performed sequence analysis using bioinformatics tools (e.g
- BLAST, Sequencer)
- Managed time efficiently in order to complete all tasks within deadlines
- Self-motivated, with strong sense of personal responsibility
Education
University of California - San DiegoLa Jolla, CA
Master of Science from Biology
07.2006 - 12.2007
University Overview
GPA: 3.96
- Thesis: A Zebrafish Transgenic Model to Study Antigen Presentation
University of California - San DiegoLa Jolla, CA
Bachelor of Science from Molecular Biology
08.2002 - 06.2006
University Overview
GPA: 3.55
- Sigma Chi Member
Skills
Interpersonal communication and teamwork skills developed through group collaboration
Analysis, interpretation and presentation of data collected through independent experimentation
Develop and clinically validate novel ELISA assays
Transition assays into commercial products
Trouble shoot quality control failures and manufacturing of product reagents
Mammalian Tissue Culture: sterile technique, culture maintenance, cryo-preservation, lipophilic transfection, recombinant protein expression, and production of hybridomas
PCR: primer design, optimize and troubleshoot stringency, gel electrophoresis, product purification and subcloning
Recombinant DNA Techniques: DNA/RNA extraction and purification, cloning and vector construction, site-directed mutagenesis, BLAST sequence analysis
Plant tissue culture practices, zebrafish care and husbandry, microinjection, whole mount in situ hybridization, fluorescence and light microscopy, spectrophotometry, Western blot analysis
Project Management: Review and approve Data Transfer Agreements, organize sample storage and workflow planning, analyze and report data, customer correspondence and satisfaction
Scientific Writing: peer reviewed publications, NIH grant writing (awarded Direct-to-Phase II 1R44DK135139-01, pending Phase I GRANT13849059), method comparison whitepapers, scientific conference poster design and presentation
Publications
- Gutschow P, Han H, Olbina G, et al. Clinical Immunoassay for Human Hepcidin Predicts Iron Deficiency in First-Time Blood Donors. J Appl Lab Med. 2020 Sep 1;5(5):943-953.
- Heeney MM, Guo D, De Falco L, Campagna DR, Olbina G, Kao PP, Schmitz-Abe K, Rahimov F, Gutschow P, et al. Normalizing hepcidin predicts TMPRSS6 mutation status in patients with chronic iron deficiency. Blood. 2018 Jul 26;132(4):448-452.
- Gutschow P, Schmidt PJ, et al. Competitive enzyme linked immunosorbent assay specific for murine hepcidin-1: correlation with hepatic mRNA expression in established and novel models of dysregulated iron homeostasis. Haematologica. 2015; 100(2): 167-177.
- Valerie Wittamer, Julien Y Bertrand, Patrick W Gutschow, David Traver. Characterization of the mononuclear phagocyte system in zebrafish. Blood. 2011;117(26): 7126-7135.
Poster Presentations
- Mark Westerman, Vaughn Ostland, Huiling Han, Patrick Gutschow, et al. Development and Early Validation of a New Competitive ELISA Highly Specific for Murine Hepcidin-1 in Serum and Urine. Poster session presented at: Bio-Iron 2013. Fifth Congress of the International Bioiron Society. 2013 April 14-18; London, England.
- Gutschow P., Westerman K., Han H., et al. Validation of the Intrinsic Hepcidin IDx™ Test, an LDT for Clinical Hepcidin Assessment. Poster session presented at: ASH 2016. 58th Annual Conference of the American Society of Hematology; 2016 Dec 3-6; San Diego, CA.
- Huiling Han, MD, Keith Westerman, BS, Vaughn Ostland, PhD, Patrick Gutschow, MS, et al. A Novel Dual Monoclonal Sandwich ELISA for Human Erythroferrone. Poster session presented at: ASH 2016. 58th Annual Conference of the American Society of Hematology; 2016 Dec 3-6; San Diego, CA.
- Patrick Gutschow, MS, Huiling Han, MD, Gordana Olbina, PhD, et al. A Novel Sandwich ELISA to Quantify Erythroferrone in Mouse Serum. Poster session presented at: ASH 2019. 61st Annual Conference of the American Society of Hematology; 2019 Dec 7-10; Orlando, FL.
Availability
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