Research scientist with over 14 years of successful experience and training in biomedical research, mainly focused on developing therapy utilizing the expertise of molecular biology, cell biology, immunology, genomics, and RNA biology. My research interests and contributions are highly relevant to studying human diseases cardiovascular, pulmonary, and inflammatory diseases. Developed target delivery of modified RNA (mod-IVT) and AAV9 gene therapy, Mitochondrial replacement therapy, and genetic modification (CRISPR) technology to mitigate heart, lung, and inflammatory diseases.
Maintaining different mice lines and generating tissue and cell-specific knockout and overexpressing inducible and non-inducible mice
Gene Therapy: in-vivo delivery
Protocol Developed
Molecular Biology
Isolation and purification of DNA/RNA, Mod-mRNA synthesis (IVT), Invitro translation, MeRIP assay Sanger Sequencing, Genotyping, Microsatellite Marker Epigenetics regulation of DNA/RNA (methylation), Real-time PCR, MicroRNA assays, RNA Splicing, Protein-RNA interaction, Immuno-precipitation (IP), Dot blot, Knockdown (siRNA, shRNA), Molecular Cloning, Site-directed mutagenesis, Crispr cas9 system, Adenovirus/Lentivirus/AAV handling, Immunohistochemistry (IHC), Immunofluorescence (IF), Protein purification, SUNset assay, Western Blotting, EMSA, ELISA, a Luciferase reporter assay, Ribo trapping, RNA immunoprecipitation (RIP assay), CHIP assay, FACS analysis, RNA sequencing (Sample preparation), cell proliferation and death assay, DNA damage (Comet Assay), Tunel Assay, Oxidative stress and ROS production, Mitochondrial DNA damage, Bioenergetics (Seahorse assay)
Cell isolation and culture
Immune cells: Expertise in isolation and culturing different immune cells (Macrophage, T cells, splenocyte, dendritic (DCs), Plasmolysis dendritic cells pDCs Alveolar Macrophage, BMDM, Neutrophils, lymphocytes, Dendritic cells, T naïve cell BMDEM, etc) Cardiomyocyte and fibroblast Neonatal and adult cardiomyocyte, fibroblast isolation, and culture Knockout and stable cell line generation: Different knockout and overexpressing stable cells were generated Cell line handled: Different human, rat, and mouse cell lines were handled Exosome: isolation, measurement, labeling, and transduction Isolation and characterization of mitochondria
Microscopy: Fluorescence and confocal microscopy
Animal model:
Endotoxin shock, intraperitoneally (LPS), and heart-killed P acne
Cecal Ligation Puncture (CLP) model for sepsis
DSS-induced colitis, EAE etc
Lung injury and fibrosis: chemical-induced lung injury, Bleomycin induced lung fibrosis
Animal model of heart diseases: Ischemic Injury/Reoxygenation (I&R), Myocardial infarction (MI), and Transverse aortic constriction (TAC), AngII, Isoproterenol, Measure heart function (Echocardiogram, Vevo)
Bioinformatics and computer proficiency for data analysis Vevo, Blast, Gene mapper, IPA, KEGG, Heat map, Statistical analysis, BioEdit, Codon Code Aligner, Lasergene Core Suite, Arlequin, MEGA, CLC workbench, DNASP, NETWORK, HaploView, Sequence data analysis, MODELLER for homology or comparative modeling of three-dimensional protein structure etc
Writing
Book Chapter
Dubey, PK, Dubey, S, Gahlawat, SK, and Kataria, RS (2021) Toll- Like Receptor in livestock species Advances in Animal Disease Diagnosis, CRC Press LLC, Taylor & Francis, Chapter 5, Page no 50-58
AWARDS AND HONORS