PRIYANKA BAPAT
Palo Alto,CA
Summary
Biologist and a researcher with over 3+ years of professional experience specializing in laboratory research, in vitro assays, and cell-based assays. Committed, results-driven and collaborative scientist with experience in cancer molecular biology, fungal biology and genome editing.
Overview
16
16
years of professional experience
Work History
Postdoctoral Research Fellow
Revolution Medicines
09.2021 - Current
- Led a team to manage project milestones
- Pursued independent and complementary research interests. Independently designed and executed a cancer signaling pathway research project by utilizing established and innovative experimental procedures to evaluate mechanisms of action of novel precision oncology targets.
- Enhanced research quality by designing and executing rgorous experiments in a timely manner.Knowledge and hands-on experience with cell-based assay development like phosphoprotein MSD, AlphaLisa, cell viability assays, combination synergy studies, and qPCR
- Troubleshooted and optimized workflows for PIP3 lipid extraction and ELISA, optimized difficult to detect antibodies for immunoblots
- Experienced in live cell imaging with Incucyte, fluorescence, and brightfield microscopy
- Routinely performed mammalian cell culture and collaborated with CRO’s to generate mutant cell lines
- Performed data analysis using tools such as Graphpad Prism, Licor imaging, Image J, Biorender, and maintained Benchling ELN’s to document data and protocols
- Collected, analyzed data, and routinely communicated insights to key stakeholders within the organization and at major conferences
- Working knowledge of lentiviral transfection and tumor organoid
- Working knowledge of reference databases and datasets like DepMap and cBioPortal
- Collaborated with multidisciplinary team members to accomplish research goals.
Graduate Student Researcher
University of California, Merced
01.2016 - 08.2021
- Independently designed and executed several multi-year novel research projects from conception to publications that have been heavily focused on assay development and improvement
- Routinely performed RNA and DNA isolation, utilized QC platforms like Qubit, Nanodrop, Agilent Bioanalyzer
- Experienced in NGS library preparation
- Performed forward genetics and genome-wide approaches like RNA-seq and ChIP-seq to identify an interwoven transcriptional network of chlamydospore formation in fungal pathogen C albicans
- Routinely designed primers and developed CRISPR-Cas9 genome editing to create GFP tagged Candida albicans transcription factor and gene knock out strains
- Developed novel non-drug therapeutic approach using photodynamic therapy with photosensitizing compounds against multi drug-resistant C albicans and C auris biofilms
- Mentored junior team members fostering their growth as future researchers and collaborated with lab members to complete project deliverables
- Managed and maintained laboratory including inventory of stocks and supplies, safety documentation, inspection scheduling, SOP creation and maintenance, and lab relocation.
- Preseented research findings at conferences and symposia showcasing the significance of work to a wider audience.
Teaching Fellow
University of California, Merced
01.2016 - 05.2018
- Courses taught: The Cell, Bacterial Pathogenesis, Microbiology.
Graduate Research Assistant
University of Missouri-St. Louis
02.2011 - 08.2013
- Performed in vitro experiments for understanding the mechanism of action and characterization of antiviral drug candidates against Human Papilloma Virus
- Routinely utilized common molecular biology and biophysical techniques like DNAse footprinting, DNA and RNA extraction, gel electrophoresis, UV-Visible Spectroscopy, Dynamic Light scattering, aggregation and formulation studies
- Managed and maintained laboratory including inventory of stocks and supplies
- Mentored a team of three junior team members.
Lecturer
University of Mumbai, India
06.2009 - 07.2010
- Courses Taught: Molecular Cell Biology, Industrial Microbiology.
Research Intern
Biogenomics Pvt. Ltd, India
05.2008 - 09.2009
- Synthetic cloning and expression of envelope glycoprotein 160 (gp160) of HIV-1 in a prokaryotic system to be used for diagnostic ELISA.
Education
Ph.D. - Quantitative and Systems Biology
University of California, Merced
Merced, CA08.2021
M.S. - Biochemistry and Biotechnology
University of Missouri-St. Louis
St. Louis, MO05.2012
M.S. + B.S. - Biotechnology
University of Mumbai, India
05.2009
Skills
- Laboratory techniques
- Experimental Work Plans
- Teaching experience
- Problem-Solving
- Research Methodologies
Presentations
- The RAS(ON) multi selective inhibitor RMC-7977 blocks downstream MAPK and PI3K pathway activation in KRASG12X-mutant cancers, American Association for Cancer Research, San Diego, April 2024
- Discovering the chlamydospore regulatory network in Candida albicans, Candida and Candidiasis meeting, March 2021
- Discovering the regulatory network controlling a unique morphology in pathogenic fungus Candida albicans, Molecular Cell Biology seminar, University of California, Merced, Nov 2019
- Discovering the chlamydospore regulatory network in Candida albicans, University of California, Berkeley, Microbiology Symposium, March 2019
- Discovering the chlamydospore regulatory network in Candida albicans, Bay Area Microbiology and Pathogenesis Symposium, University of California, San Francisco, March 2019
- Discovering the chlamydospore regulatory network and light sensing in Candida albicans, Northern California Branch Annual Society for Microbiology, Pleasanton, CA, March 2018
- Discovering the transcriptional network controlling chlamydospore formation and light sensing in Candida albicans, Bay Area Microbiology and Pathogenesis Symposium, University of California, San Francisco, March 2017
Awards
- QSB USAP fellowship (Univ of California, Merced) Summer 2019
- Distinguished Scholars Fellowship (Univ of California, Merced) 2018
- Graduate Student Marshal awarded to outstanding student (Univ of Missouri-St. Louis) May 2012
Publications
- The RAS(ON) multi selective inhibitor RMC-7977 blocks downstream MAPK and PI3K pathway activation in KRASG12X-mutant cancers, Manuscript in preparation
- An interwoven transcriptional regulatory network controls chlamydospore formation in Candida albicans, Manuscript in preparation
- A Computational Workflow for Analysis of 3' Tag-Seq Data, Current Protocols 3 (2): e664, 2023
- Photodynamic therapy is effective against Candida auris biofilms, Frontiers in Cellular and Infection Microbiology. 11:713092, 2021
- AddTag, a two-step approach with supporting software package that facilitates CRISPR/Cas-mediated precision genome editing, G3: Genes, Genomics and Genetics. 11(9), 2021
- Visible lights combined with photosensitizing compounds are effective against Candida albicans biofilms, Microorganisms, 9, 500, 2021
- The protein kinase Ire1 impacts pathogenicity of Candida albicans by regulating homeostatic adaptation to endoplasmic reticulum stress, Cellular Microbiology, e13307, 2021
- In Vitro Culturing and Screening of Candida albicans Biofilms, Current Protocols in Microbiology. Vol 50, Issue 1, e60, 2018
- Distinct roles of the 7-transmembrane receptor protein Rta3 in regulating the asymmetric distribution of phosphatidylcholine across the plasma membrane and biofilm formation in Candida albicans, Cellular Microbiology.19: e12767, 2017
References
References available upon request
Timeline
Postdoctoral Research Fellow
Revolution Medicines
09.2021 - Current
Graduate Student Researcher
University of California, Merced
01.2016 - 08.2021
Teaching Fellow
University of California, Merced
01.2016 - 05.2018
Graduate Research Assistant
University of Missouri-St. Louis
02.2011 - 08.2013
Lecturer
University of Mumbai, India
06.2009 - 07.2010
Research Intern
Biogenomics Pvt. Ltd, India
05.2008 - 09.2009
Ph.D. - Quantitative and Systems Biology
University of California, Merced
M.S. - Biochemistry and Biotechnology
University of Missouri-St. Louis
M.S. + B.S. - Biotechnology
University of Mumbai, India
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