Rafaella Bezerra de Lima Henrique
Redmond,WA
Summary
Quality Control Scientist with a PhD in Biological Sciences and extensive hands-on experience in cell-based assays, fluorescence/confocal microscopy, nanoparticle uptake studies, and quantitative data analysis. Strong foundation in GLP/BSL‑2 laboratory compliance, aseptic technique, and precise documentation practices. Skilled in flow cytometry data analysis (FlowJo) and experienced in developing, optimizing, and executing cell characterization assays. Brings meticulous attention to detail, strong troubleshooting ability, and readiness to operate in GMP-regulated QC environments, supporting in‑process testing, release assays, and analytical method development for biologics products.
Overview
4
4
years of professional experience
Work History
PhD in Biological Sciences
Nano Lab
, Brazil
04.2022 - 12.2022
- Executed cell-based assays with glucose-derived fluorescent nanoprobes in breast cancer models, ensuring reproducibility and adherence to GLP standards.
- Performed quantitative analysis and data validation through flow citometry and microscopy imaging, aligning with QC documentation practices.
- Strengthened scientific writing and reporting accuracy via manuscript preparation and structured experimental records.
Researcher
Nano Lab
, Brazil
08.2018 - 04.2022
- Conducted cell culture, including 2D monolayers and 3D spheroids, ensuring viability through assays and spheroid formation via hanging drop technique.
- Prepared buffers, media, and chemical solutions adhering to GLP and biosafety guidelines.
- Operated laboratory equipment, including biosafety cabinets, CO₂ incubators, centrifuges, spectrophotometers, fluorescence and confocal microscopes, ultrasonic baths, and micropipettes.
- Performed nanoparticle labeling and uptake experiments, conducting fluorescence imaging and quantitative analysis.
- Maintained lab organization by managing inventory control, waste disposal, sterilization of materials, and equipment cleaning.
- Recorded experimental data accurately in lab notebooks while assisting in troubleshooting protocols.
- Supported undergraduate students and research teams with routine laboratory tasks and experimental procedures.
M.Sc. in Biological Sciences
NanoBio Lab
, Brazil
03.2020 - 03.2022
- Developed 3D spheroids from human cervical adenocarcinoma cells and performed nanoparticle uptake studies.
- Executed experimental protocols, analyzed results, and contributed to scientific publications.
Education
PhD - BIOLOGICAL SCIENCES
UFPE - PERNAMBUCO FEDERAL UNIVERSITY
01-2024
M.SC. - BIOLOGICAL SCIENCES
UFPE - PERNAMBUCO FEDERAL UNIVERSITY
01-2022
B.SC. - BIOMEDICINE
UFPE - PERNAMBUCO FEDERAL UNIVERSITY
01-2019
Skills
- Quality Control & Compliance: GLP, GMP awareness, BSL‑2 biosafety procedures, documentation accuracy, deviation reporting, SOP adherence
- Analytical Techniques: Fluorescence and confocal microscopy, spectrophotometry, quantitative imaging analysis
- Cell-Based Assays: 2D and 3D cell culture, spheroid formation, viability assays, uptake assays
- Sample & Material Handling: Sterilization, autoclaving, waste management, buffer and solution preparation
- Equipment Operation: CO₂ incubators, biosafety cabinets, centrifuges, spectrophotometers, ultrasonic baths, micropipettes, general QC instrumentation
- Documentation & Data Integrity: Lab notebook management, experimental recordkeeping, inventory control
- Laboratory Support: Sample preparation, routine testing, troubleshooting protocols, maintaining controlled environments
Languages
- Portuguese (Native)
- English (Fluent)
Additional Academic Experience
American Journal of Life Sciences (2025-present), Archives of Cancer Science and Therapy, Colloids and Surfaces B: Biointerfaces, World Journal of Clinical Oncology, Biological Regulators and Homeostatic Agents, Current Gene Therapy, Published peer-reviewed articles and conference abstracts., Recipient of multiple academic awards and honorable mentions.
Timeline
PhD in Biological Sciences
Nano Lab
04.2022 - 12.2022
M.Sc. in Biological Sciences
NanoBio Lab
03.2020 - 03.2022
Researcher
Nano Lab
08.2018 - 04.2022
PhD - BIOLOGICAL SCIENCES
UFPE - PERNAMBUCO FEDERAL UNIVERSITY
M.SC. - BIOLOGICAL SCIENCES
UFPE - PERNAMBUCO FEDERAL UNIVERSITY
B.SC. - BIOMEDICINE
UFPE - PERNAMBUCO FEDERAL UNIVERSITY