Statistical and Data Analysis: R, IBM SPSS, SAS, JMP, Graph Pad Prism, Excel (advanced data analysis techniques, pivot tables, data visualization), Power Bi Office Suite: Microsoft Word, Microsoft PowerPoint, Microsoft Access, Microsoft Publisher
Ranjit Kumar Das
Summary
Aspiring Research Scientist with a solid foundation in biotechnology and molecular biology, cultivated through extensive graduate-level research and hands-on laboratory experience. Over 7 years of academic involvement, demonstrating proficiency in SOPs, laboratory techniques, and data analysis. Known for exceptional organizational and time management skills, effectively balancing research responsibilities with teaching duties. Collaborative team player recognized for strong problem-solving abilities and commitment to excellence. Eager to transition into a research scientist role within the pharmaceutical industry, aiming to contribute innovative solutions and positive societal impacts.
Overview
11
11
years of professional experience
Work History
Graduate Research Assistant
Dr. Roy's Lab
- Current
- Planned and conducted comprehensive research and analysis to examine the role of STAT1 and IFIT3 Genes in HIV-Associated Neurocognitive Disorder (HAND) and Alzheimer's disease
- Utilized RT-PCR for gene expression analysis, specifically STAT1 and IFIT3 in SH-SY5Y cells
- Performed immunocytochemistry to observe the expression of STAT1 and IFIT3 gene expression as well as immunohistochemistry to observe the expression of IFIT3 gene expression in APP/PS1 mice model
- Used ImageJ for quantification of IFIT3 and STAT1 gene and protein expression
- Conducted Western blot analysis to examine STAT1 and IFIT3 protein expression in SH-SY5Y, Human Astrocytes cells, and Mice tissue
- Performed multiplex ELISA assays to measure proinflammatory cytokines in response to HIV-1 virus and antiretroviral drugs
- Executed P24 assays for assessing HIV-1 viral load in Humanized mice and human astrocytes
- Executed cytotoxicity assays on mammalian cells, assessing cell viability post-treatment with Tat, amyloid beta, and cART drugs; findings informed the development of novel therapies targeting specific cellular mechanisms
- Measured reactive oxygen species (ROS) in SH-SY5Y cells after exposure to Tat protein, cART, and amyloid beta
- Isolated human peripheral blood mononuclear cells (PBMCs) from blood samples
- Used Olympus Flowview FV10i Confocal microscope for capturing the Immunocytochemistry and Immunohistochemistry images
- Extracted DNA and RNA from mammalian cells and mice tissue
- Conducted literature reviews, managed time and resources of technical staff, and guided and supervised bachelor students in laboratory practicals and internships
- Animal model: Conducted experiments using Humanized NSG (hu-NSG) mouse models, managing a cohort of 35 mice
- Administered HIV-1 virus via intraperitoneal injection and tested various HIV-1 drugs using tail vein injections
- Performed blood collection through Mandibular and Cardiac puncture techniques
- Developed strong skills in detailed dissection and organ harvesting for further analysis.
Graduate Research Assistant
Professor Asim Dutta Roy's Lab
01.2018 - 01.2020
- Conducted comprehensive analysis to determine the dose-dependent effect of fruitflow consumption on platelet function and blood pressure
- Identified the cardioprotective properties of a Norwegian raspberry
- Prepared fruit extract using freeze-dried method and platelet-rich plasma from Human blood sample
- Conducted platelet aggregation study using different types of berries
- Determined IC50 using different fruit extractions (Raspberry, Kiwino)
- Measured platelets factor-4 (PF4) using the PF4 assay kit using a HuPF-4 ELISA kit
- Developed and executed platelet aggregation assay using ADP and performed platelet counting via DxH 900 Hematology analyzer
- Executed Thromboxane B2 assays to measure platelet activation and investigate aspirin efficacy, contributing to research on antiplatelet therapy
- Developed collaborations and communicated technical information about platelets and nutrition research with a variety of individuals, including government officials, scientific and academic communities, etc.
Graduate Research assistant
Robert Wilson Lab
01.2015 - 01.2018
- Determination of the Eukaryotic organism content in Roe Deer rumen by using 18s ribosomal RNA (rRNA) sequencing
- Performed DNA extraction from the Roe deer intestinal tissue
- Prepared library for illumina sequencing
- Extracted protein from rumen tissue of the roe deer and performed SDS-APGE gel electrophoresis
- Analyzed sperm cell properties by Microscopy, Computer-assisted sperm analysis (CASA), and flow cytometry
- Performed sperm motility, viability, and acrosomal integrity test
- Carried out metagenomic analysis of Infant gut microbiota
- Investigated the change in infant gut microbiota from 10 to 90 to 360 days of life.
Molecular Biologist
Chevron clinical laboratory
06.2013 - 05.2015
- Coordinated and scheduled the day-to-day activities of the laboratory personnel
- Led team in molecular testing of virus and bacterial infections
- Led the validation of extraction and detection kits for viral RNA, ensuring accuracy and reliability in RNA extraction and detection processes
- Performed RT-PCR machinery optimization
- (Roche light cycler-96, Rotor Gene-Q, Bio-Rad- CFX96) Wrote/provided Standard Operating Procedure (SOP) and on-the-job training to new employees and monitored team performance.
Education
MSc in Biochemistry and Molecular Biology -
University of Texas Rio Grande Valley (UTRGV)
USA, Expected May 2024MSc in Experimental and Commercial Biotechnology -
Inland Norway University of Applied Sciences (INN)
Norway, 2018BSc in Biochemistry and Biotechnology -
University of Science and Technology Chittagong (USTC)
Bangladesh, 2013training certificates
- IACUC animal handling training, University of Texas Rio Grande Valley (UTRGV), 2022
- Radiation and Protection and Radioactivity, Department of Chemistry, University of Oslo, 2019
Software
honors awards
- Poster presentation on The Gerontological Society of America 2022 conference, Indianapolis, USA
- Poster presentation on American Aging Association conference 2023, Oklahoma, USA
- Consecutive 3rd time UTRGV School of Integrative Biological and Chemical Sciences scholarship
skills techniques
Molecular Biology: Agarose Gel Electrophoresis, SDS PAGE, Western Blot, RT-PCR, Purification of DNA, Illumina Sequencing Sample Preparation and Data Analysis.
Cellular Biology: Mammalian Cell Culture, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Protein Extraction and purification.
Animal Husbandry: Mouse Injections (Intramuscular, Intraperitoneal, and Tail Vein Injection), Harvesting of Tissues and Organs, and Blood Collection.
Microscopy: Phase Contrast Microscopy, Fluorescent Microscopy, Confocal Microscopy.
Hematological Assay: Platelet Aggregation Assay, Platelet Counting Via Dxh 900 Hematology Analyzer, Thrombin Generation Assay.
publications
- Evaluation of the equivalence of different intakes of Fruitflow in affecting platelet aggregation and thrombin generation capacity in a randomized, double-blinded pilot study in male subjects, Das, R.K., Datta, T., Biswas, D., BMC Nutr, 2021, https://doi.org/10.1186/s40795-021-00485-5
- Extracts of tamarillo, horned melon, and raspberry, but not extract of pear, inhibit human blood platelet aggregation: Investigating the underlying factors for their differential mechanisms, Agnese Barin, Ranjit K. Das, Nasser E. Bastani, Per Ole Iversen, Asim K. Duttaroy, Journal of Functional Foods, 2023, https://doi.org/10.1016/j.jff.2023.105847
References
References available upon request.
Timeline
Graduate Research Assistant
Professor Asim Dutta Roy's Lab
01.2018 - 01.2020
Graduate Research assistant
Robert Wilson Lab
01.2015 - 01.2018
Molecular Biologist
Chevron clinical laboratory
06.2013 - 05.2015
Graduate Research Assistant
Dr. Roy's Lab
- Current
MSc in Biochemistry and Molecular Biology -
University of Texas Rio Grande Valley (UTRGV)
MSc in Experimental and Commercial Biotechnology -
Inland Norway University of Applied Sciences (INN)
BSc in Biochemistry and Biotechnology -
University of Science and Technology Chittagong (USTC)
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