Raul Pena
Northridge,CA
Summary
I am an N-COMPASS Scholar seeking a full-time internship position in the field of biology where I can not only apply my current knowledge and skills but gain more as well. My current lab experience includes aseptic technique, micropipetting, and plasmid purification. I am a very versatile worker and am able to work well alone or with others.
Overview
2
2
years of professional experience
Work History
Intern
Pathways To Stem Cell Science
07.2023 - 08.2023
- Assisted students doing experiments
- Monitored students to insure proper use of materials
- Aliquot materials for students before experiments
- Conducted procedure on counting muscle stem cells
General Merchandiser
Target
08.2022 - 05.2023
- Responsible for maintaining assigned department organized and all products are in stock for the customer’s convenience
- Back up cashier and back up for online pickup orders
- Assisted customers while on the sales floor
Utility Clerk
Superior Grocers
08.2021 - 07.2022
- Responsible for a variety of roles which were assigned
- Roles include: Parking lot clerk, porter, dairy deli frozen clerk, grocery clerk, meat department clerk, and cashier
- Responsible for providing excellent customer service while making sure the store was safe and in proper condition for customers to shop in
Education
Bachelor of Science - Biotechnology
California State University - Northridge
Northridge, CA05.2025
Skills
- Cell Culture
- Molecular Biology
- Cell culture media preparation
- Mammalian suspension/ Adherent cell line culture
- Cell Counts with hemocytometers
- Thaw and Cryopreserve cell lines
- Passaging cells/ seed by confluency and quantity
- Epifluorescence microscopy
- Cell prep for Flow Cytometry
- Transient transfection GFP
- Stem cell growth MEF feeder and matrigel
- Plasmid DNA extraction
- Professional Skills
- Gel Electrophoresis and PCR
- Subcloning and Bacterial Transformation
- Restriction digest analysis
- SDS-PAGE and Western Blotting
- CRISPR-Cas 9 BioRad
- Preparing and pouring selective LB agar plates
- Bacterial manipulation
- Aseptic Technique
- Laboratory Safety
- Teamwork Abilities
- Time Management
Cell Culture Training
Pathways Advance Stem Cell Techniques
-Derive NSC from hiPSC
-Culture NSC monolayers and neurospheres
-Characterize and differentiate NSC
-Culture, characterize and differentiate MSC
-Culture HIPSC/ESC in MTESR and NutriStem
-Pick and passage hiPSC/hESC colonies
-Passage hiPSC/ESC/Accutase and ReLeSR
-Electroporate NPSCINESC
-Cryopreserve and thaw hiPSC/ESC Characterization for immuncingcal markers, vector integration and pluripotency
- Prepare embryoid bodies (scraped/spin-EB's)
- Differentiate HiPSC/ESC to germ layers
-Quality control monitor hiPSC/hiESC culture
- Electroporate INPSC/MESC
-Comply with ESCRO regulations
- Perform cloning assays
- Reprogram hiPSC/ plasmids, Sendi Virus
- Select, prepare, aliquot hiPSC/hESC reagents
-Morphologically assess hiPSC/hESC colonies
References
Dr. Cindy Malone
Professor of Biology
Director of CIRM N-COMPASS
cmalone@csun@csun.edu
Dr. Victoria Fox
President and Executive Director of Pathways to Stem Cell Science
drvictoriafox@outlook.com
Timeline
Intern
Pathways To Stem Cell Science
07.2023 - 08.2023
General Merchandiser
Target
08.2022 - 05.2023
Utility Clerk
Superior Grocers
08.2021 - 07.2022
Bachelor of Science - Biotechnology
California State University - Northridge
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