RIMA HOSSARY

I worked in the Blood Bank department, where I analyze blood and perform the following tests:

• Type and Screen
• Antibody Identification panels
• Elutions
• Cross match appropriate PRBCs
• Cord blood type and direct Coombs test
• Prepare blood products in a timely manner for patients in need
• Perform the necessary maintenance and quality control procedures for reagents and analyzers.

• I worked in the Immunology and Molecular Biology department where I test for a variety of infectious agents, such as COVID 19, influenza A and B, Trichomonas vaginalis, Neisseria gonorrhoeae, Chlamydia trachomatis, MRSA using PCR on the following instruments BD Max, Panther, Cepheid, Roche 6000, and BioFire
• I am also trained to operate the following analyzers: Diasorin, Optilite, and Inova DSX (which is automated ELISA)
• I performed manual ELISA to identify Heparin Induced Thrombocytopenia antibodies along with various other manual tests such Cyptococcal antigen, RPR, VDRL on CSF, and stool lactoferrin and H
• Pylori; most of which are lateral chromatography tests
• I was the unofficial lead of the evening shift as there was no appointed one
• My supervisor trusted me with projects such as collecting specimens for validation studies, overseeing the evening shift workflow and staff as a whole and reporting back to her with any problems or any improvement ideas.

• I worked in Hematology, Coagulation, Urinalysis, Chemistry and Blood Bank:
• Hematology:
• I ran CBC and reticulocyte count on the Beckman Coulter DxH machine
• I performed peripheral blood and bodily fluid differentials under the microscope
• I performed ESR, sickle cell screening and Kleihauer-Betke test
• Coagulation:
• I ran PT, PTT, Fibrinogen and D Dimer tests and their respective QC on the ACL elite machine
• Urinalysis:
• I ran urinalysis on the Siemens Clinitec analyzer and read the urine specimens that were positive for blood, leukocytes, protein and nitrogen under the microscope
• Chemistry:
• I ran a variety of tests on the Beckman Coulter DXC machine including but not limited to glucose, potassium, magnesium, chloride, direct bilirubin, total bilirubin, hemoglobin A1C, BNP, troponin, cholesterol, triglycerides, variety of drug tests on both serum and urine
• I ran QC and calibrate the reagents when necessary.

• I worked in Hematology, Coagulation, Urinalysis, Chemistry, Blood Bank and some microbiology and I am also trained in phlebotomy:
• Hematology:
• I ran CBC and reticulocyte count on the Beckman Coulter LH series machine
• I read differentials and body fluids under the microscope
• Coagulation:
• I ran PT, PTT, D dimer, fibrinogen on ACL machine and analyzed the results accordingly
• Urinalysis:
• I ran urinalysis on the Siemens Clinitec analyzer and read the urine specimens that were positive for blood, leukocytes, protein, ketones and nitrogen under the microscope
• Chemistry:
• I ran a variety of tests on the Siemens Vista machine including electrolyte, hepatic, lipid panels among others
• I ran QC, calibration, and maintenance as necessary
• Blood Bank:
• I ran type and screen on the ORTHO Provue instrument utilizing the gel method
• I performed immediate spin crossmatches using the tube method and full crossmatches with the gel method
• I performed antigen typing, elution, saline replacement, prewarm, cold panel, direct coombs test, transfusion reaction, emergency release of blood products
• I thawed fresh frozen plasma, cryoprecipitate
• I ordered platelets and performed immediate spin and full cross matches accordingly on PRBCs and allocated the unit (s) to the appropriate patient while paying close attention to the attributes and/or antibodies the patient may possess
• I ran reagent and equipment QC
• Microbiology:
• I performed Gram stain and am able to identy Gram positive and negative cocci and bacilli under the microscope
• I ran PCR tests using GeneXpert instrument in order to identify MRSA and Clostridium difficile
• I ran Influenza A,B and RSV rapid identifying assays
• Phlebotomy:
• I greeted patients and identify myself and the purpose of my visit
• I asked for their consent to draw blood
• After getting their consent, I asked patients to verbally state their name and date of birth and compare that information with my label
• I then compared their ID band with my label checking their name, date of birth and medical record number
• I paid close attention to limb alert bands, wounds, hematomas and/or bruises as well as which arm has an IV line
• I proceeded with finding the best possible vein and drawing the blood in the most painless and uncomfortable way possible for my patient
• I used the correct order of drawing tubes and label my tubes according to the test (s)requested.

• I worked in Hematology, Coagulation, Urinalysis, Chemistry and Blood Bank:
• Hematology:
• I ran CBC and reticulocyte count on the Beckman Coulter DxH machine
• I performed peripheral blood and bodily fluid differentials under the microscope
• I performed ESR, sickle cell screening and Kleihauer-Betke test
• Coagulation:
• I ran PT, PTT, Fibrinogen and D Dimer tests and their respective QC on the ACL elite machine
• Urinalysis:
• I ran urinalysis on the Siemens Clinitec analyzer and read the urine specimens that were positive for blood, leukocytes, protein and nitrogen under the microscope
• Chemistry:
• I ran a variety of tests on the Beckman Coulter DXC machine including but not limited to glucose, potassium, magnesium, chloride, direct bilirubin, total bilirubin, hemoglobin A1C, BNP, troponin, cholesterol, triglycerides, variety of drug tests on both serum and urine
• I ran QC and calibrate the reagents when necessary.

• I was trained to use the Starlims software and I am now a proficient user
• I worked in the processing laboratory in the Serology section for one month where I aliquoted Zika, MMRV and HIV specimens into 2 tubes, one for testing and one for archive
• I was responsible for aliquoting positive and equivocal Zika specimens for ship out to Wadsworth Center
• I organized all the tested Zika specimens in boxes by accession number
• I helped coat the plates with goat anti human IgM for Zika IgM ELISA testing
• I collected over 100 specimens for validation testing of the new Serology machine called Agility
• I helped the technologists find specimens from the archive as well as from the boxes of the tested specimens.

• I translated doctors appointments between doctors and nurses and non english speaking patients.

• I worked in the Coagulation laboratory
• We mainly tested for PT and PTT, fibrinogen, plus certain coagulation factors.

• I rotated in the following laboratories in AUBMC:
• Hematology: I manually stained the slides with Wright and Giemsa stain
• I performed over a 100 CBCs and differential analyses
• I read the automated CBCs and differential analyses done by the Beckman Coulter DX1 machine
• I observed and assisted the technologists along with the pathologists while using the Flow Cytometry analyzer as well as observed the analysis of the results to help with the diagnosis of certain leukemias such as CLL and ruling out others
• Chemistry: I processed the specimens coming in
• Under the supervision of a technologist, I used the Cobas 6000 machine to complete the required tests
• Semen Analysis: I performed all the steps required for semen analysis and examination
• Urine Analysis:
• I conducted all the steps required for collection, transportation, and macroscopic examination of urine
• I performed all biochemical investigation on different urine specimens (reagent strip and other tests)
• I performed all microscopic investigations on different urine samples (Crystals in urine, parasites in urine)
• I examined synovial fluid under a polarized microscope
• Parasitology:
• I conducted the different techniques for identification of intestinal parasites, Protozoa
• Nematodes, Trematodes and Cestodes
• Microbiology: I prepared slides to be read under the microscope using the Gram stain
• I inoculated the tubes and the agars accordingly and read the results that allowed me to identify the bacteria
• I also performed antimicrobial susceptibility tests
• Cytogenetics: I performed the procedure leading up to a karyotype
• Molecular Biology: I only observed the procedures the technologists perform
• Histology: I only observed how tissue is processed and the procedure leading up to preparing slides
• Serology: I performed latex agglutination, immune-chromatography testing, and indirect and direct hemagglutination tests
• I performed an ELISA HIV detection test under the supervision of a technologist
• Blood Bank: I processed the blood donors’ paperwork and guided them to filling the necessary forms
• I took their blood pressure, weight, temperature, and hemoglobin level
• I performed:
• ABO blood type tests
• Rh typing
• Weak D testing
• Direct and indirect antihuman globulin tests
• Immediate spin cross match
• Full cross match

• I ran type and screen, weak D, antigen typing and panels on the Galileo Echo machine utilizing the solid phase testing method
• I manually performed direct coombs test, weak D, fetal screen, elution, cold panel, antigen typing, saline replacement, prewarm, emergency release of blood products and transfusion reaction workup
• I thawed fresh frozen plasma, cryoprecipitate
• I ordered platelets and performed immediate spin and full cross matches accordingly on PRBCs and allocated the unit (s) to the appropriate patient while paying close attention to the attributes and/or antibodies the patient may possess
• I ran reagent and equipment QC
• I've helped improve the SOPM by suggesting a few modifications
• For example, I suggested that the doctor be made aware of the dangerous implications of transfusing a doe patient and be made to sign a form that outlines those risks
• I've also recommended performing a full cross match for doe patients even if the antibody screen was negative since we do not have their full medical history
• I worked closely with the Blood Bank Lead to evenly distribute daily tasks as well as to ensure all work was flowing smoothly and any problems were promptly tackled
• I was handpicked by the Blood Bank supervisor to be her lead but due to the long commute and my ongoing master’s practicum at the time I had to unfortunately decline
• Microbiology:
• I ran Influenza A, B and RSV identifying assays using the GeneXpert PCR instrument
• I ran rapid HIV identifying assay., :
• I ran type and screen, weak D, antigen typing and panels on the Galileo Echo machine utilizing the solid phase testing method
• I manually performed direct coombs test, weak D, fetal screen, elution, cold panel, antigen typing, saline replacement, prewarm, emergency release of blood products and transfusion reaction workup
• I thawed fresh frozen plasma, cryoprecipitate
• I ordered platelets and performed immediate spin and full cross matches accordingly on PRBCs and allocated the unit (s) to the appropriate patient while paying close attention to the attributes and/or antibodies the patient may possess
• I ran reagent and equipment QC
• I've helped improve the SOPM by suggesting a few modifications
• For example, I suggested that the doctor be made aware of the dangerous implications of transfusing a doe patient and be made to sign a form that outlines those risks
• I've also recommended performing a full cross match for doe patients even if the antibody screen was negative since we do not have their full medical history
• Microbiology:
• I ran Influenza A, B and RSV identifying assays using the GeneXpert PCR instrument
• I ran rapid HIV identifying assay.

• Tel: 212-447-2910
• Email: myounis@health.nyc.gov
• Rhodora Basea, 447-2909
• Email: rbasea@health.nyc.gov

• 2916
• Email: syassim@verizon.net
• Dr
• Sami Ramia

• Ext: 4698
• Fax: 961-1-744470
• Email: sramia@aub.edu.lb
• Issam Shafi'
• MLS Seniors'

• Ext: 5207
• Email: is05@aub.edu.lb

• 350000
• Fax: 961-1-744470
• Ext
• 4697
• Email: ka28@aub.edu.lb
• Rolla Khatib

• 350000
• Fax: 961-1-744470
• Ext: 4696
• Email: ra03@aub.edu.lb
• Dr
• Rami Mahfouz

• Of the Molecular Diagnostics Laboratory (Histocompatibility and Molecular Pathology) Director of the Flow Cytometry and Clinical Hematology Laboratory, 5167
• Email: rm11@aub.edu.lb

• Ext: 5216
• Email: rh25@aub.edu.lb
• In collaboration with the Infection Prevention leadership develops, implements, and evaluates the organizational infection prevention program
• Conducts surveillance of hospital acquired infections in accordance with CDC's National Healthcare Safety Network (NHSN) definitions
• Supervises coordinates and participates in activities of employees performing routine standardized biological and biochemical tests as well as more complex procedures on blood that assist physicians in the diagnosis and clinical management of patients, assumes responsibility for recognizing results or problems that require referral to Medical Director or Laboratory Director or Manager, Performs additional tasks as requested by the Chair of Pathology or his designee