Rinki Dasgupta

Genome Editing : Participated in research projects by designing multiplex PCR ,qRT PCR , in vitro whole genome based CRISPR editing protocols and executing experiments.

Drug Discovery : Designed and interpreted high throughput screening results using IncucyteS3 ,multiplex Flow Cytometry assisted Cell Sorter to screen small molecules.

Subcellular organelles organization : Coordinated and managed laboratory projects by isolating subcellular organelles , enabling microfluidic Nano Sight technology with Horiba Size Analyzer isolated organelles extracellular vesicles characterized .

LC-MS Skills : High throughput screening of small molecules uptake by subcellular organelle using Shimadzu's High Performance Liquid Chromatography.

High content Image analysis : Quantitative imaging for tracking subcellular response; apply machine learning to large datasets, and explore advanced imaging modalities like super-resolution microscopy to achieve a more comprehensive validation loop

Big Omics tools and Auto Dock ;Leveraging cornputational data to identify target small molecules for prediction of potential ligand-receptor binding to design experimental priorities.

Scientific Writing : Partnered with PI to assist in writing grant proposals that generated $368,000 NINDS NIH funding for the lab.
Collaboration : Served as primary contact with other researchers, increasing collaboration and communication efficiency by 32%.

Lab Skills : “High-throughput screening of small molecules using the IncuCyte S3 platform and multiplex flow-cytometry–assisted cell sorting.”

Genome editing :Optimized in vitro CRISPR-mediated gene editing , refining sgRNA design and golden gate cloning ,transfection, and validation of CRISPR edits with TIDE analysis using NGS data.

Data Analysis: Oversaw project data and lab-database update cycles, achieving a 99 % data-accuracy rate.

Scope: Provided comprehensive medical care to infants, children, and adolescents.
Responsibilities: Conducted thorough assessments, diagnostics, and therapeutic planning.
Patient Care: Offered outpatient and inpatient services, including examinations, diagnosis, medication prescriptions, vaccinations, and developmental guidance.
Specialized Rotations: Excelled in high-intensity rotations such as pediatric intensive care, neonatal intensive care, and pediatric emergency care, dedicating over 80 hours per week.
Tenure: Successfully served as a resident pediatric physician for a year, leaving a lasting impact on patient well-being and medical excellence