Rohana Liyanage
Fayetteville,AR
Summary
Determined and well-rounded individual with more than 30 years working experience as a mass spectrometry Scientist. Sharp and focused professional offering skills in proteomics, metabolomics and lipidomics.
Overview
36
36
years of professional experience
Work History
Senior Scientist
University Of Arkansas At Fayetteville
01.2002 - Current
- Provide routine mass spectrometry sample analysis for structure determination and purity by using Direct infusing ESI-MS, LC-ESI-MS, GC-MS (Both low and high resolution)
- Provided research support to more than 100 undergraduate students and graduate students planning and executing experiments to maximize quality
- Teach more than 200 graduate and undergraduates mass spectrometry by conducting workshops and classes
- Simultaneously managed several complex projects, meeting all pertinent milestones
- Performed complex troubleshooting and assisted in leading initiatives to solve challenging scientific problems with assays or instrumentation
- Evaluated and implemented new technologies and laboratory techniques to improve quality and robustness of sample analysis
- Collaborated in writing more than ten proposals to obtain instruments and also in research projects involved in mass spectrometry research
Postdoctoral Research Associate
University of Utah
06.1996 - 01.2002
- Conducted research on gas phase ion chemistry to obtain bond energies and reaction cross sections on transition metals and their clusters using guided ion beam tandem mass spectrometry
Research and Teaching Assistant
University Of Illinois, UIC
09.1990 - 04.1996
- Study electronic structure perturbations in HCl/DCl using 2+1 REMPI-TOF-MS
- Conducted laboratories and tutorial classes in general and physical chemistry
Assistant Lecturer
University of Colombo
05.1989 - 06.1990
- Conducted lectures and laboratories in physical chemistry f
- Reviewed teaching activities and identified and implemented strategies to improve student learning outcomes.
Research Chemist
Medical Research Institute
01.1987 - 01.1990
- Conducted research on antibacterial and antifungal compounds in natural products
Education
Ph.D. - Electronic Structure And Perturbations in HCl/DCl
University of Illinois At Chicago
Chicago, IL06.1996
Bachelor of Science - Chemistry
University of Colombo
Colombo, Sri Lanka06.1987
Skills
- H/D exchange mass spectrometry/platform to study protein folding/energies, protein-ligand/protein-protein interactions, protein 3D structure
- Native electrospray mass spectrometry of proteins to study protein and ligand binding interactions
- Limited proteolysis mass spectrometry to study protein 3D structure and accessibility of amino acid residues
- Top down and bottom-up mass spectrometry characterization of antibodies
- LC-ESI-MS (nano, capillary), GC-EI-MS, LC-MALDI-TOF-MS (nano, capillary), TLC-MALDI-TOF-MS
- Targeted/untargeted proteomics, lipidomics and metabolomics
- Bacterial identification and characterization using both protein and lipid profiles using MALDI, ESI, and MAIV MS
- 1D/2D gel electrophoresis and in-gel digestion of proteins for MALDI/ESI mass spectrometry characterization
- Tissue imaging of peptides, proteins, and lipids by MALDI-TOF-MS
- Quantification and identification of fatty acids by using FAMES (fatty acid methyl esters) from complex lipid samples by GC-MS
- Configuring and managing in house protein servers and protein databases
- Familiar with skyline, Mascot, Mascot distiller, scaffold, and scaffold PTM software which are highly valuable in automating protein identification, quantification, and biological pathway determinations
Accomplishments
- Developed New HDX method (PEPS-HDX-MS) to study protein folding
- Developed limited proteolysis MALDI-TOF-MS methodologies to study protein 3D structure
- Developed gel base LC-MS/MS methodologies to improve the protein identifications and quantification from plasma and urine
- Developed methodologies to extract phenolic metabolites from plasma and urine and quantify by using LC-MS MRM triple quadrupole mass spectrometry
- Developed methods to identify biological conditions and systems with minimum sample preparation by using Direct MALDI-TOF-MS, MAIV-FTMS including bacteria.
- Developed methods to understand trapping ions in side ICR cell in FTMS using polyethylene glycol polymers
Affiliations
1994 - 1998 American Chemical Society (ACS),
2002 - Present American Society for Mass Spectrometry (ASMS) member
Timeline
Senior Scientist
University Of Arkansas At Fayetteville
01.2002 - Current
Postdoctoral Research Associate
University of Utah
06.1996 - 01.2002
Research and Teaching Assistant
University Of Illinois, UIC
09.1990 - 04.1996
Assistant Lecturer
University of Colombo
05.1989 - 06.1990
Research Chemist
Medical Research Institute
01.1987 - 01.1990
Ph.D. - Electronic Structure And Perturbations in HCl/DCl
University of Illinois At Chicago
Bachelor of Science - Chemistry
University of Colombo
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