Romario Pusey
New Haven,CT
Summary
Detail-oriented Research Assistant with hands-on experience performing experimental and laboratory work to advance research goals. Drives team success with expertise in data review, reporting and specimen handling. Specializes in Tissue Engineering, Biomaterials and Medical Device Research.
Overview
8
8
years of professional experience
Work History
Research Assistant
Biomaterials and Medical Device Innovation Laboratory
08.2022 - 05.2023
- Co-led research on antithrombotic Artificial Lungs development, Liposomes as drug carrier nanoparticles, Mamalian Cellular proliferation and cytotoxicity, and Hydrogels as immunotherapeutic biomaterials.
- Regularly operated devices such as the Avant Liposome Preparation Kit, Biotek Plate reader, UV-Vis-Spectrophotometer, UVO-Cleaner, Nitric Oxide Analyzer, Floid Cell Imaging Microscope, Leica Microscope, Micropipettes, CO2 Incubator, Scanning ElectronMicroscopy (Trained at Yale University).
- Conducted ELISA and WST assays including Fibrinogen Adsorption and Lactate Dehydrogenase for material thrombosis screening.
- Coated Artificial Lungs using anti-thrombogenic Polysulfobetaine Methacrylate and Dopamine and evaluated their antifouling activity.
- Investigated Liposome encapsulation with Nitric Oxide,co-cultured and passaged Cells with Liposome with Nitric Oxide donor, and tested fluorescence activity of Liposome using Carboxyfluorescein diacetate.
- Prepared and analyzed different concentrations of Hydrogels.
- Monitored Nitric Oxide release from biomaterials using the Sievers 280i Nitric Oxide Analyzer and Lab View Software.
- Research article writing and publication in peerreviewed journals and conference proceedings(https://onlinelibrary.wiley.com/doi/10.1002/ mabi.202200479).
- Extensively used R-studio for statistical analysis, Image J viewer for cellular analysis, and Microsoft Excel for Statistical Analysis.
- Conducting Chromatography techniques for Protein purification in the production of Liposome.
- Prepared presentations on research findings for conferences.
- Developed methods for collecting and organizing qualitative data such as focus groups or open-ended interviews.
- Placed orders for laboratory equipment and supplies
- Inspected finished products for quality and compliance.
- Managed multiple research projects simultaneously while meeting deadlines efficiently.
- Collected, reviewed, and analyzed primary source materials to identify relevant information for projects.
- Adhered to and followed quality standards to preserve integrity of data and findings.
- Conducted tests on equipment and instruments for quality.
Research Lab Volunteer
Biomaterials and Medical Device Innovation Laboratory
10.2021 - 05.2022
- Prepared presentations on research findings for conferences or seminars
- Developed methods for collecting and organizing qualitative data such as focus groups or open-ended interviews
- Placed orders for laboratory equipment and supplies
- Inspected finished products for quality and compliance
- Contacted and coordinated testing with contract laboratories and vendors
- Managed multiple research projects simultaneously while meeting deadlines efficiently
- Collected, reviewed, and analyzed primary source materials to identify relevant information for projects
- Adhered to and followed quality standards to preserve integrity of data and findings
- Shadowed seasoned student researchers on Liposome Projects, Bacteriorhodopsin projects, and Nitric Oxide release projects
- Designed experiments to characterize fluorescein-conjugated Goat-Igg to human Fibrinogen
- Performed literature reviews to identify current trends in the field being studied
- Compiled data into tables, graphs, charts for presentation purposes or publication in journals or magazines
- Monitored and reported on research progress and related activities
- Utilized various library resources to locate relevant sources for research projects
- Conducted data analysis and interpretation of research results, utilizing statistical software packages such as Minitab, and Excel
- Maintained accurate records of all project activities including documentation of experimental results and conclusions drawn from them
- Created detailed reports summarizing the findings from qualitative and quantitative studies
- Adhered to and followed quality standards to preserve integrity of data and findings
- Cultured Mamalian Cells to test against Nitric Oxide drugs loaded in Liposomes
- Conduct WST assays for Lactate Dehydrogenase studies to analyze protein fouling on Medical Devices
- Conduct Fibrinogen ELISA assays on biomaterials for Protein characterization.
Science Teacher
Bustamante High School
01.2019 - 08.2021
- Taught subjects in the science area including Chemistry and Integrated Science, instructed Chemistry Laboratory Activities, and advised students
- Managed Organic Chemistry Lab to ensure all safety standards were followed
- Prepared reagents and other apparatus for Laboratory procedures
- Conducted Organic processes such as saponification, electrophilic aromatic substitution reaction, hydrocarbon production, product purity testing, and synthesis of amines and amides Compounds
- Tutored students on various topic areas of Organic Chemistry
- Collaborated with Lecturers and other instructors to carry out time-constraint tasks.
Organic Chemistry Lab Instructor
Northern Caribbean University
09.2015 - 01.2018
- Prepared materials, solutions and chemicals for experiments
- Managed Organic Chemistry Lab to ensure all safety standards were followed
- Prepared reagents and other apparatus for Laboratory procedures
- Conducted Organic processes such as saponification, electrophilic aromatic substitution reaction, hydrocarbon production, product purity testing, and synthesis of amines and amides Compounds
- Tutored students on various topic areas of Organic Chemistry
- Collaborated with Lecturers and other instructors to carry out time-constraint tasks.
Education
Master of Science - Biomedical Engineering
University of New Haven
West Haven, CT05.2023
Bachelor of Science - Biology
Northern Caribbean University, Manchester
Mandeville, Jamaica12.2017
Skills
- Medical Device Research
- Problem-Solving
- Biosafety Level-2
- Cell culture Techniques
- R-Software & Minitab
- Microsoft Office Suites
- Lab View
- Cell Staining
- Live Cell Imaging
- Cell Growth with Aseptic Technique
- Serial Dilution
- Attention to Detail
- Enzyme Linked Immunosorbent Assay (ELISA)
- Flow Cytometry
- Drug Discovery
- Assay Development
- Biocompatibility Testing
- Tissue Engineering
- Quality Control
- Technical Writing
- Project Management
- Biomedical Instrumentation
- Polymerase Chain Reaction
- Microscopy and Fluorescence Microscopy
- Publications
- Quality Assurance
- Research Paper Writing
- Research Reporting
- Statistical Analysis
- Specimen and Sample Preparation
- Data Entry
- Analytical Thinking
- Matlab
- Research and Publication
- Conducting Assays
- Equipment Maintenance
Timeline
Research Assistant
Biomaterials and Medical Device Innovation Laboratory
08.2022 - 05.2023
Research Lab Volunteer
Biomaterials and Medical Device Innovation Laboratory
10.2021 - 05.2022
Science Teacher
Bustamante High School
01.2019 - 08.2021
Organic Chemistry Lab Instructor
Northern Caribbean University
09.2015 - 01.2018
Master of Science - Biomedical Engineering
University of New Haven
Bachelor of Science - Biology
Northern Caribbean University, Manchester
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