Roshan Karthik Nandhakumar
Bryan,TX
Summary
Master of Biotechnology student at Texas A&M University with hands-on experience in molecular cloning, recombinant protein expression, and immunological assays. Skilled in optimizing enzymatic processes and contributing to research projects, including vaccine development, with a focus on practical problem-solving and clear communication.
Overview
2
2
years of professional experience
Work History
Student Research Assistant
School of Public Health, Texas A&M University
10.2024 - Current
- Conducted Hemagglutination Inhibition (HI) assays on100+ field-collected samples to detect influenza antibodies, adhering to biosafety and testing protocols
- Experienced in preparation and standardization of reagents and samples, including handling and processing serum, virus control antigens, and RBC solutions for immunological assays
Research Associate
Kcat Enzymatic Private Limited
07.2023 - 06.2024
- Optimized process parameters across100+ experiments spanning four projects, resulting in 25% increase in recombinant enzyme expression employing E. coli BL21(DE3) expression system
- Engineered targeted single nucleotide mutations in enzyme-coding genes leveraging PCR mediated site-directed mutagenesis
- Documented and presented project results and analyses to team members, senior management, and clients, ensuring clear communication for effective decision-making
- Procured and maintained laboratory inventory worth10,000 USD, including plastic consumables, media, chemicals, and other essential lab accessories ensuring no resource related work disruptions
Research Intern
Kcat Enzymatic Private Limited
12.2022 - 04.2023
- Performed necessary molecular biology techniques such as Plasmid isolation, Transformation and Electrophoresis30+ times to support protein expression and characterization studies
- Collaborated in the execution of enzyme expression in K. pastoris, ensuring precision and accuracy in all tasks, leading to error-free and reliable results
Education
Master of Biotechnology (PSM) -
Texas A&M University
College Station, TX05-2026
Bachelor of Technology - Biotechnology
PSG College of Technology
College Station, TX04.2023
Skills
TECHNICAL
- Molecular Cloning
- Cell Culture
- Recombinant Protein Expression
- Polymerase Chain Reaction (PCR)
- Gel Electrophoresis (Agarose & SDS-PAGE)
- Western Blotting
- Plasmid Isolation
- DNA & RNA Extraction
- Competent Cell Preparation
- Protein Purification
- HI Assay
-
PROFESSIONAL:
- Good Laboratory Practices (GLP)
- Client Care
- Analytical Writing
- Communication
- Scientific Presentation
- Data collection
- Microsoft365
- Inventory Management
LEADERSHIP ROLES AND VOLUNTEER WORK
- College Biotechnology Association | Executive (Nominated): Oversaw a team of50+ for planning, budgeting, and advertising, for over20 core and non-core, intra- and inter-collegiate events, technical workshops, and guest lectures increasing overall participation by15% to over700 students and staff.
07/2021 - 05/2022
- National Service Scheme (NSS) | Member: Volunteered for clean-up and renovation activities benefitting2 schools and over30 houses of families living under the poverty line as part of NSS, an initiative organized by Indian Ministry of Youth Affairs and Sports.
08/2019 - 05/2020
- High school Interact club | Vice-president (Elected): Spearheaded10+ cultural programs, social awareness marches and donation drives for local orphanages and old age homes, as a core committee member of my school Interact club (School wing of Rotary International).
06/2017 - 06/2017
Timeline
Student Research Assistant
School of Public Health, Texas A&M University
10.2024 - Current
Research Associate
Kcat Enzymatic Private Limited
07.2023 - 06.2024
Research Intern
Kcat Enzymatic Private Limited
12.2022 - 04.2023
Master of Biotechnology (PSM) -
Texas A&M University
Bachelor of Technology - Biotechnology
PSG College of Technology
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