RAHUL TRIPATHI
Montgomery
Summary
Innovative Postdoctoral Researcher specializing in computational biology and machine learning. Expertise in high-dimensional data integration, spatial transcriptomics, and local LLMs for clinical data extraction. Skilled in characterizing tumor immune microenvironments through WGCNA and spatial gradient analysis, with a solid foundation in molecular cloning and pathway overexpression.
Overview
8
8
years of professional experience
Work History
Postdoctoral Research Associate
Alabama State University
Montgomery
01.2026 - Current
- Developed and deployed local LLMs for automating clinical data extraction of Perineural Invasion (PNI) status from 501 pathology reports, prioritizing direct nerve involvement.
- Integrated high-dimensional datasets by building end-to-end pipelines with edgeR/limma, MOFA2, and WGCNA to identify novel regulatory drivers and biomarkers.
- Executed spatial deconvolution and spatial gradient analysis via nerve marker profiling to map cellular architecture and characterize the tumor immune microenvironment.
- Executed spatial gradient analysis and nerve marker profiling to map cellular architecture and characterize tumor immune microenvironment stratified by PNI.
- Designed tumor-focused co-expression networks by filtering datasets to isolate PNI-driven regulatory modules, establishing a computational basis for identifying new therapeutic targets.
Senior Research Fellow
Indian Institute of Wheat and Barley Research
Haryana
12.2017 - 12.2018
- Characterized 112 Loose Smut and 59 Karnal Bunt isolates through DNA extraction, PCR assays, and microscopy, enhancing understanding of pathogen diversity across geographical regions.
- Conducted population genetic structure analysis using STRUCTURE 2.3.4, AMOVA, and linkage disequilibrium (LD), contributing to insights on molecular variation and evolutionary dynamics.
- Contributed to genomic pipeline development for pathogen identification, performing genome-wide SSR mining for Loose Smut (Ustilago segetum tritici), Covered Smut (Ustilago hordei) and Karnal Bunt (Tilletia indica).
- Managed experimental data repositories and biological cultures, including plant sample cultivation for pathogen confirmation, resulting in two co-authored manuscripts in Frontiers in Microbiology.
- Mentored 5 trainees by overseeing technical protocols and providing feedback on workflows, fostering skill development and experimental rigor.
Education
Ph.D. - Biotechnology
Delhi Technological University
Delhi, India12-2025
M.Tech. - Biotechnology
Motilal Nehru National Institute of Technology
Prayagraj, Uttar Pradesh, India12-2016
B.Tech. - Biotechnology
N.C. College of Engineering (Kurukshetra University)
Panipat, Haryana, India12-2010
Skills
- Bioinformatics Analysis
- Omics Integration
- Genomic Engineering
- Translational Research
- Machine Learning
- Artificial Intelligence
- Large Language Models
- Spatial analysis
- Big Data Management
- Systems Biology
- Cell Culture
Publications
- Identification of CXCR4 Inhibitory Activity in Natural Compounds using Cheminformatics-guided Machine Learning Algorithms, Integrative Biology, 01/01/25, Tripathi R, Kumar P, 10.1093/intbio/zyaf004, First-Author Research Publication, 17
- Preliminary study to identify CXCR4 inhibitors as potential therapeutic agents for Alzheimer's and Parkinson's diseases, Integrative Biology, 01/01/23, Tripathi R, Kumar P, 10.1093/intbio/zyad012, First-Author Research Publication, 15
- Free radical biology in neurological manifestations: mechanisms to therapeutics interventions, Environmental Science and Pollution Research, 01/01/22, Tripathi R, Gupta R, Sahu M, Srivastava D, Das A, Ambasta RK, Kumar P, 10.1007/s11356-021-16693-2, First-Author Review, 29
- Unwinding the modalities of necrosome activation and necroptosis machinery in neurological diseases, Ageing Research Reviews, 01/01/23, Gupta R, Kumari S, Tripathi R, Ambasta RK, Kumar P, 10.1016/j.arr.2023.101855, Co-Author Publication
- Protein S-sulfhydration: Unraveling the prospective of hydrogen sulfide in the brain, vasculature and neurological manifestations, Ageing Research Reviews, 01/01/22, Gupta R., Sahu M, Tripathi R, Ambasta RK, Kumar P, 10.1016/j.arr.2022.101579, Co-Author Publication
- Cross talk mechanism of disturbed sleep patterns in neurological and psychological disorders, Neuroscience & Biobehavioral Reviews, 01/01/22, Sahu M, Tripathi R, Jha NK, Jha SK, Ambasta RK, Kumar P, 10.1016/j.neubiorev.2022.104767, Co-Author Publication
- Protective role of anticancer drugs in neurodegenerative disorders: A drug repurposing approach, Neurochemistry International, 01/01/20, Advani D, Gupta R, Tripathi R, Sharma S, Ambasta RK, Kumar P, 10.1016/j.neuint.2020.104841, Co-Author Publication
- Mitochondrial Dysfunction in Metabolic Disorders, Mitochondrial Dysfunction and Nanotherapeutics, 01/01/21, Advani D, Sharma S, Tripathi R, Gupta R, Jaiswal A, Ambasta RK, Kumar P, 10.1016/B978-0-323-85666-9.00015-2, Co-Author Book Chapter
Timeline
Postdoctoral Research Associate
Alabama State University
01.2026 - Current
Senior Research Fellow
Indian Institute of Wheat and Barley Research
12.2017 - 12.2018
Ph.D. - Biotechnology
Delhi Technological University
M.Tech. - Biotechnology
Motilal Nehru National Institute of Technology
B.Tech. - Biotechnology
N.C. College of Engineering (Kurukshetra University)