Rutuja Kulkarni
Gaithersburg,MD
Summary
Versatile and results-driven Clinical Laboratory Assistant with a robust background at Luminis Health Doctors Community Medical Center, excelling in PCR testing and regulatory compliance. Demonstrated expertise in mammalian cell culture and aseptic techniques, alongside a collaborative spirit in research environments. Proven track record in enhancing laboratory efficiency and accuracy, ready to leverage skills in a dynamic new role.
Overview
3
3
years of professional experience
Work History
Clinical Laboratory Assistant
Luminis Health Doctors Community Medical Center
03.2024 - Current
- Carried out weekly quality control (QC) testing, and calibrated laboratory instruments to ensure regulatory compliance.
- Maintained laboratory supplies for routine and emergency testing.
- Conducted PCR tests (GI, RESP, COVID, MI/CSF PCR) using IVD kits, following established protocols.
- Performed phenotypic identification assays, bacterial identification, and antimicrobial susceptibility testing (including BCID).
- Prepared and examined smear preparations, performed culture plating, and utilized spreading plate techniques for bacterial isolation and identification.
- Evaluated microbiological culture plates, and assisted in pathogen identification.
- Expertise in LIMS software (Epic) for proper specimen tracking, accessioning, labeling, and recording.
- Processed and shadow complex tissue samples, including biopsy, frozen tissue, and cytology fluids, for cancer studies.
- Assisted in the collection, handling, and storage of biological tissue samples.
- Performed tissue embedding and sectioning using a microtome for microscopic analysis.
- Conducted Hematoxylin and Eosin (H&E) staining, along with specialized staining techniques (IHC, PAS, Giemsa, histochemical stains).
- Ensured compliance with CLIA, HIPAA, OSHA, and safety guidelines.
- Eligible for ASCP certification, MTS/MLS, and participated in ongoing training.
Research Assistant
Symbiosis School of biological Sciences
Pune, India
05.2022 - 10.2022
- Maintained and cultured the mammalian cell line using advanced aseptic techniques, ensuring optimal cell growth.
- Executed and analyzed cell-based potency assays, ELISA, MTT, MTS, and Western Blot, delivering precise data interpretations.
- Monitored cell survival post-treatments, ensuring data validity by using flow cytometry.
- Performed titration and prepared a potent bacteriophage stock tailored for a specific E. coli.
- Actively assisted undergraduate students in their academic research presentations, guidance in DOE, execution, and data analysis.
- Utilized Microsoft Office, Microsoft Excel, and JMP for streamlined data analysis and reporting.
- Collaborated closely with a diverse team of researchers, students, and faculty in research based on immunology.
Junior Embryologist
Rainbow IVF Center
Agra, India
02.2022 - 05.2022
- Conducted human semen analysis, DFI, vitality test, semen morphology, and preparation of semen samples for ICSI, IUI, and IVF.
- Assisted with oocyte pickups, performed embryo transfer, and embryo biopsy.
- Interpretation and analysis of clinical reports of biopsy studies, and maintained records using Excel and AI tools.
- Performed cryopreservation of human semen, human eggs, and human embryos.
- Conducted independent research on various infertility issues in males and females.
Education
Master of Science - Biotechnology
Northeastern university
Boston, MA12.2023
Master of Science - Biotechnology
Symbiosis International University
India06.2021
Bachelor of Science - Biotechnology
Rajarshi Shahu College of Biotechnology
India04.2019
Skills
- Mammalian cell culture
- Cryopreservation
- Aseptic cell culture
- Primary cell lines handling
- Virus sample handling
- Bacterial culture preparation
- Bacteriophage isolation, purification, characterization
- DNA isolation, RNA isolation
- Protein isolation and purification
- qPCR, ddPCR
- Western blot analysis
- SDS-PAGE
- Primer designing for PCR
- Gold nanoparticles preparation, characterization
- ELISA
- Flow cytometry assay
- Electrophoresis
- MTT assay, MTS assay
- Electron microscopy
- Immunofluorescence
- Confocal microscopy
- BLAST
- RasMol
- PyMOL
- Chimera
- Microsoft Excel
- Microsoft PowerPoint
- JMP software
- DOE tools
- Phenotypic identification assays
- Bacterial identification assays
- Antimicrobial susceptibility testing
- LIMS Epic software
- Hematoxylin and eosin staining, PAS staining,Giemsa staining
- Histochemical staining
- Tissue embedding
Academic Projects
Gold Nanoparticle-Mediated Overcoming of P-glycoprotein Efflux in Triple Negative Breast Cancer: The Therapeutic Potential R6G in conjugation with R6G- Aunps and Verapamil
Department of Pharmaceutical Sciences, Northeastern University, Boston, USA
Formulated and evaluated Gold Nanoparticles for multidrug-resistant breast cancer treatment, Investigated nanoparticle and verapamil impact on MDR1 production in MDR-MB-231 cells via sandwich ELISA, Purified IgG protein from fetal bovine serum using affinity chromatography, detected with SDS-PAGE, Studied particle uptake and colocalization with mitochondria and MDR1 by fluorescence microscopy, Extracted RNA from cultured MDR-MB-231 cells, amplified RNA signal through cDNA synthesis and PCR, Possesses research skills in mammalian cell culturing, Western Blot, Affinity Chromatography, SDS-PAGE, RNA Isolation, RT-qPCR, MTT Assay, and more.
Influence of Bacteriophages on Survival, Activation and Phagocytic Activity of Macrophages,
Symbiosis School of Biological Sciences, Pune, India
Isolated and characterized novel bacteriophage against E. coli to check its immunological response on macrophage cell line, Cultured BALB/c mice cells and U937 macrophage cell line to check immune response after induction of bacteriophage, Performed Animal handling and bleeding and isolation of PBMC, Established flow cytometry based assay to check macrophage cell survival and performed antibody-detecting techniques such as Western Blot, Performed immunological cell-based potency assays and analytical assays like Sandwich ELISA, MTS to check cell survival and cytokine profile
Timeline
Clinical Laboratory Assistant
Luminis Health Doctors Community Medical Center
03.2024 - Current
Research Assistant
Symbiosis School of biological Sciences
05.2022 - 10.2022
Junior Embryologist
Rainbow IVF Center
02.2022 - 05.2022
Master of Science - Biotechnology
Northeastern university
Master of Science - Biotechnology
Symbiosis International University
Bachelor of Science - Biotechnology
Rajarshi Shahu College of Biotechnology
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