Overview
Work History
Education
Skills
Poster presentations
References
Websites
Timeline
Generic

SAILESH PHUYAL

Dunbar,WV

Overview

8
8
years of professional experience

Work History

Microbiology Research Technician

West Virginia State University
, USA
08.2024 - Current
  • River sediment sample collection and preparation
  • Laboratory Equipment Operation (GC, ICP-OES, HPLC, IC), DNA Extraction, quantification, Gel Electrophoresis, PCR
  • Library preparation; downstream processing of microbiome DNA and Data Analysis (R, JMP, and STAMP)

Graduate Research and Teaching Assistant

West Virginia State University
, USA
08.2022 - 07.2024
  • DNA Extraction, quantification, Gel Electrophoresis, qPCR
  • Metagenomics in K-Base; Qiime-2 for 16S rRNA gene
  • R-studio, R, Conda, Cytoscape, JMP, STAMP
  • Taught and oversaw microbiology lab experiments for different groups of nursing students

Application Specialist

Axis Medibiz Pvt. Ltd
Kathmandu, Nepal
03.2020 - 06.2022
  • Provided application and operational training on the proper use of microbial diagnostic machines such as BacT/ALERT 3D, Vitek 2 compact, and RTPCR machines from Biofire and MolBio
  • Ensured customer satisfaction and troubleshooting

Secondary Level School Teacher, (Part-time)

Malpi City School
Kathmandu, Nepal
01.2017 - 03.2022
  • Planned and delivered engaging lessons, fostering a positive and inclusive learning environment
  • Organized and supervised extracurricular activities contributed to the overall development of students
  • Maintained open communication with parents, providing regular updates on student progress

Education

Master of Science - Biotechnology (Environmental Microbiology)

West Virginia State University
West Virginia
07.2024

B. Tech - Biotechnology

Kathmandu University
Dhulikhel, Nepal
10.2015

Skills

Bioinformatics and computational skills
  • QIIME 2
  • K-Base
  • RNA and DNA seq analysis
  • DESeq2
  • Enhanced Volcano
  • R programming
Molecular Biology Techniques
  • PCR
  • DNA/RNA extraction and quantification
  • Gel electrophoresis
  • NGS library preparation
  • PCR-based gene cloning
Protein analysis and cell culture
  • Western blotting
  • ELISA
  • Mammalian cell culture
  • Protein purification
Microbiology and culture techniques
  • Bacterial media preparation
  • Culture techniques
Analytical Chemistry Techniques
  • Gas Chromatography
  • HPLC
  • ICP-OES
  • Ion Chromatography
Transferable/soft skills
  • Scientific writing
  • Presentations
  • Collaboration
  • Teamwork
  • Performance under pressure

Poster presentations

  • Xenobiotic Metabolism in the Kanawha-New River (WV) Watershed: Impacts of Land-Use Practices, Phuyal S, FM Guerrero-Toledo, Gonzalez-Rangel MC, Bhattarai B, Hass A, Rojano F, Huber DH, Association of 1890 Research Directors (ARD) Research Symposium, 04/24, Nashville, TN

References

  • David H. Huber, Professor of Biology (Research Advisor), huberdh@wvstateu.edu
  • Vijay Gurung, Global Customer Service, Lab Automation (Supervisor), vijaygurung@biomerieux.com

Timeline

Microbiology Research Technician

West Virginia State University
08.2024 - Current

Graduate Research and Teaching Assistant

West Virginia State University
08.2022 - 07.2024

Application Specialist

Axis Medibiz Pvt. Ltd
03.2020 - 06.2022

Secondary Level School Teacher, (Part-time)

Malpi City School
01.2017 - 03.2022

Master of Science - Biotechnology (Environmental Microbiology)

West Virginia State University

B. Tech - Biotechnology

Kathmandu University

Similar Profiles

CREATE PROFILE
SAILESH PHUYAL