Samantha Deavila

· Creating and distributing departmental event schedules and flyers for Journal Club, Seminars and "Named Lectures.”

· Coordinating travel arrangements for guest speakers and lecturers to visit our department

· Generating meeting minutes and agendas for monthly faculty meetings

· Administering departmental software licenses to faculty, staff, and students for Graphpad Prism and Biorender.

· Ordering scientific artwork of high resolution images for department display including a commerorative centennial plaque for the Doisy Research Center lobby.

· Coordinating interviews with SLU’s CORE Program Ph.D. candidates on behalf of the Training Committee.

· Involved in the organization of the Inaugural and second Doisy Conference on Coagulation in Saint Louis

· Uploading quotes and documents for review in Agiloft, SLU’s contract system, for capital equipment purchases, collaborations, material transfer agreements, etc.

· Placing electronic orders through SLU information technology services (ITS).

Purified blood coagulation proteins in common pathway using HPC4 immunoaffinity chromatography and FPLC techniques.

Obtained crystal structure of wild type thrombin bound to FPF_cmk substrate.

Conducted active site titration experiments with Hirudin for protein activation assessment.

Performed Western Blots to analyze protein expression levels.

Executed cell culture on BHK adherent cells to maintain viable samples.

Screened and optimized X-ray crystallography experiments using ART Robbins instruments for crystal shipment preparation.

Surveyed alpha-thrombin mutants for kinetic analysis through substrate hydrolysis and PTT assays.

Conducted tissue culturing on tSA-201 HEK cells, including passaging and transfections.

Developed protocol for isolating sinoatrial node cells from mice for FRET labeling.

Mapped neuronal and cardiac HCN channel gene subunits using noncanonical amino acids for dual color labeling.

Prepared solutions and reagents to investigate cellular dynamics of signaling lipids via patch clamping.

Analyzed data from FLIM-FRET images to visualize membrane domains in various cell types.

Operated liquid-handling robots to achieve high-throughput production targets.

Assisted with automated steps in patient diagnostic testing under licensed CLS supervision.

Executed SOPs for Infinium assay protocols to ensure compliance and accuracy.

Conducted maintenance schedules to maintain cleanliness and proper operation of laboratory equipment.

Documented WIP in daily written reports for effective shift communication.

Managed potentially hazardous materials in a BSL 2 environment to uphold safety standards.

● Executing SOP and V&V (Verification and Validation) protocols for DOA (Drugs of Abuse) tests using lateral flow immunoassay devices.

● Conducting V&V studies including pH, temperature flex, specific gravity, cutoff characterization, stability, cross-reactivity, and endogenous and exogenous interference

● Operating laboratory equipment such as lateral flow immunoassay test readers (eCup+ system)

● Creating product builds for R&D experiments

● Documenting V&V study results and assisting senior staff with collecting and documenting data and writing reports including Design Verification Incidents

● Troubleshooting product software bugs, instrumentation malfunctions and design irregularities and discussing improvements with IT, engineering and process improvement teams

● Working in an ISO 13485 medical device, FDA regulated environment and continuously upholding quality

● Preparing gold nanoparticle-antibody conjugate solutions, buffers, and reagents

● Maintaining and purchasing laboratory supplies, equipment, and chemicals in coordination with the procurement department

● Coordinating with EH&S department to ensure lab safety, proper labeling, SDS procurement and adherence to hazardous material disposal

● Trained in Good Documentation Practice (GDP), Good Laboratory practice (GLP), Good Manufacturing Practice (GMP), Good Clinical Practice (GCP)

Conducted PCR with cDNA libraries to generate plasmids for antisense RNA riboprobes.

Created fluorescent reporter constructs through restriction enzyme-based directional cloning.

Generated transgenic ascidians to analyze cell division patterns during embryonic PNS development.

Recapitulated Neurogenin expression in Ciona robusta by comparing in situ hybridization with fluorescently labeled cells.

Fertilized ascidian eggs for electroporation of plasmid constructs and fixed embryos at various stages.

Characterized embryonic cell lineages expressing transgenic transporter genes using fluorescent microscopy.

Executed embryological protocols including gamete extraction, fertilization, electroporation, and embryo culturing.