Samuel Wieczerzak
Summary
Scientist with an educational background in Microbiology and Oceanography. 7 years of professional lab experience. Highly skilled in cell culture, environmental monitoring and training.
Overview
7
7
years of professional experience
1
1
Certification
Work History
Cell Processing Specialist
Novartis Pharmaceuticals
, NJ
04.2021 - Current
- Perform process unit operations ( cell culture, purification, aseptic processing, and cryopreservation) according to standard operating procedures and batch records, while clearly and contemporaneously recording production data and information.
- Member of Intensive Care Unit Work Cell.
- Prepare and maintain cell expansion using incubators, single use bioreactors, and cell automated equipment Operation of equipment such as: NC-200 automated cell counter, Cell Saver 5 autologous blood recovery system.
- Perform routine static environmental monitoring.
- Perform training for various processes.
- Collaborate with engineering and metrology on the calibration and maintenance of equipment.
- Highly knowledgeable in the use of production related IT systems such as LIMS, MES, SAP & UNICORN.
- Responsible for aseptic cell production operations of patient-derived clinical and commercial cellular immunotherapy Kymriah.
- Maintaining knowledge of cGMP regulations and FDA guidance applicable to biologic's and cell therapy manufacturing.
- Communicate with other departments as part of an cross-functional team to maintain high efficiency of production tasks required by shift schedule.
Cell Processing Associate
Novartis Pharmaceuticals
, NJ
12.2019 - 04.2021
- Responsible for preparation, production and storage of media and adherence to the production schedule.
- Highly knowledgeable in the use of production related IT systems such as LIMS, MES, SAP & UNICORN.
- Perform process unit operations according to standard operating procedures and batch records, and record production data and information in a clear, concise, format according to Good Documentation Practices (GDP).
- Perform training on various processes.
- Ability to gown aseptically and work in clean room environments including ISO 5,7 and 8 areas.
Microbiologist
Nubiyota LLC
, NY
10.2018 - 08.2019
- Set up and manage the company's GMP pilot plant facility.
- Responsible for performing and training Environmental Monitoring and collecting water samples.
- Responsible for training and managing a team of six employees who will be responsible or the routine activities in the pilot plant.
- Responsible for establishing relationships with vendors, as well as handling the negotiations for bulk material purchasing.
- Responsible for writing and reviewing SOP's as needed.
- Responsible for developing inventory system for laboratory items such as but not limited to consumables, raw materials and reagents.
Student Researcher
Rutgers–New Brunswick
New Brunswick, NJ
Jun 2016 - Aug 2018
- Worked one on one with the chair of Microbiology, Dr Max Haggblom and collaborators, Dr Lee Kerkhof, Dr Aamani Rupukala.
- Research: Member of the Haggblom cohort researching the degradation patterns of Pharmaceutical Personal Care Products (PPCP's) in various bodies of water that include but are not limited to, the Hudson and Raritan Rivers. Conducted water column analyses of salinity, dissolved oxygen, pH and temperature of various locations along the Raritan River. Pharmaceutical water analysis was done using LC-MS/MS and the degradation of PPCP's was tracked via RP-HPLC. DNA of active enrichment cultures and isolates was obtained via phenol chloroform extraction and purified using Ampur beads. The RNA was then prepped for sequencing with Minion by doing three stages of PCR amplification, an operon amp, an overhang amp and a barcode amp. QA/QC was done after each amplification, and a bead cleanup was done before the subsequent amplification.
- That research has been continued by other members of the lab and subsequently turned into a PhD.
- Presented research progress at Raritan River Consortium 2017.
- Presented research progress at the SEBS annual microbiology symposium 2016, 2017.
Chemistry Tutor
Middlesex County College
Edison, NJ
08.2014 - 05.2016
- Worked one on one and in groups on Basic Chemistry through Organic Chemistry class material.
- Documented student attendance and progress against goals.
Chemistry Lab Technician
Middlesex County College
Edison, NJ
01.2014 - 09.2015
- Prepared reagents for Basic, General and Organic Chemistry labs. Up to 65 labs/wk.
- Instrumentation training/maintenance on NMR, GC, IR, HPLC.
- Accurately inventoried lab chemicals and scheduled waste pick ups.
- Required Yearly RCRA Training.
- Set up and maintain gas lines for anaerobic chamber and Schlenk Lines for fume hood.
Education
Bachelor of Science - Microbiology and Oceanography
Rutgers University
New Brunswick2018
Associate of Science - Biology and Chemistry
Middlesex County College
Edison2016
Skills
- Cell Culture (7 Years)
- Aseptic Technique (7 Years)
- Training and Development (3 Years)
- High Performance Liquid Chromatography (HPLC) (3 Years)
- Gas Chromatography (GC) (2 Years)
- Thin Layer Chromatography ( 2 Years)
Certification
- Wetland Delineation from The Wetland Training Institute.
- Environmental Specialist from OSHA.
- ARC GIS, Mapping and Spatial Analysis Specialization from the University of Toronto.
- CPR & AED.
References
- Dr. Max Haggblom, Distinguished Professor and Chair of Microbiology, Rutgers University. Chief Editor of FEMS. 848-932-5646
- Dr. Aamani Rupukala, Research Scientist at Colgate Palmolive, 732-692-3861
- Dr. Phalguni Ghosh, Chair of Chemistry, Middlesex County College. 732-906-2587
- Dr. Thomas Cullen, Professor & Scientific Advisor Sandoz Pharmaceuticals. 609-627-8180
Timeline
Cell Processing Specialist
Novartis Pharmaceuticals
04.2021 - Current
Cell Processing Associate
Novartis Pharmaceuticals
12.2019 - 04.2021
Microbiologist
Nubiyota LLC
10.2018 - 08.2019
Chemistry Tutor
Middlesex County College
08.2014 - 05.2016
Chemistry Lab Technician
Middlesex County College
01.2014 - 09.2015
Student Researcher
Rutgers–New Brunswick
Jun 2016 - Aug 2018
Bachelor of Science - Microbiology and Oceanography
Rutgers University
Associate of Science - Biology and Chemistry
Middlesex County College
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