Samyuktha Kolavasi
Pflugerville,TX
Summary
Driven Senior Research Assistant with a proven track record at MD Anderson Cancer Center, showcasing expertise in immunotherapy and vaccine efficacy evaluation. Excelled in complex flow cytometry and data analysis, demonstrating meticulous attention to detail and superior project management skills. Achieved significant advancements in cancer research through innovative clinical trials and collaborative team leadership.
Overview
6
6
years of professional experience
Work History
Senior Research Assistant
MD Anderson Cancer Center
Houston, TX
11.2016 - 03.2020
- Evaluated adjuvant properties using OT-1 mice model.
- Utilized alpha-GalCer, CpG, and QS21 adjuvants in various projects.
- Administered intranasal E6/E7 peptide vaccine with adjuvants to test efficacy.
- Evaluated effectiveness of combining vaccine with immunotherapies.
- Collected minimally invasive cervical cytobrush samples during clinical studies.
- Analyzed intratumoral immune cell changes during chemoradiation through kinetic studies, and compared them with peripheral mononuclear cell kinetics.
- Assisted in configuring panels and conducting intricate 16-color panel flow cytometry using the LSRFortessa X20 device.
- Managed the lab supplies, kept inventories, and maintained a clean lab space.
- Interpreted test results and determined need for retesting.
- Attended conferences to present findings from completed studies.
- Supervised junior research assistants on project tasks.
- Participated in weekly team meetings to discuss progress on individual tasks.
- Performed administrative duties such as scheduling meetings, preparing agendas, taking notes during meetings.
Research Technician-III
Baylor College of Medicine
Houston, TX
06.2014 - 10.2016
- Conducted multiple clinical trials involving cancer patients and various treatment methods, using adoptive T cell therapies.
- Processed patient samples including peripheral blood and bone marrow.
- Isolated DNA from frozen and fresh cells, as well as biopsy tissues, using Qiagen mini prep kits.
- Conducted QPCR assay analysis to monitor transgene levels post T-cell infusion at various time points in patients.
- Produced PHA-activated T cells.
- Conducted monitoring of immune responses using PBMCs with ELISPOT assays.
- Conducted quantitative analysis of human anti-mouse IgG antibody levels in serum and plasma samples using HAMA ELISA.
- Conducted Milliplex MAP human cytokine/chemokine magnetic bead panel assay for plasma samples using Luminex 200.
- Documented clinical data for analysis.
- Managed and organized patient records across multiple databases for research purposes.
- Collaborated with research coordinators.
- Experienced with GMP environments.
Education
Masters of Science - Biotechnology
University of Houston- Clear Lake
Houston, TX05.2014
Bachelors of Pharmacy -
Jawaharlal Nehru Technological University
India06.2012
Skills
- Tissue culture
- Cell line maintenance
- Cell media preparation
- Standard Operating Procedure Compliance
- Animal work includes retro-orbital bleeding in mice, tumor induction, intranasal vaccine administration, and intra-tumoral injections
- Tissue collection
- Tumor Immune Profiling
- Surface and intracellular staining
- Flow cytometry
- ELISA
- ELISPOT
- LUMINEX 200
- QPCR
- Gel electrophoresis
- Microsoft Excel
- Microsoft PowerPoint
- EndNote proficiency
- Database searches
- Clinical trial support
- GraphPad Prism
- FlowJo analysis
- Fundamental SQL Knowledge
- Analyzing and synthesizing data
- Patient log maintenance
- Liquid nitrogen log maintenance
- Patient Follow-Up Management
Timeline
Senior Research Assistant
MD Anderson Cancer Center
11.2016 - 03.2020
Research Technician-III
Baylor College of Medicine
06.2014 - 10.2016
Masters of Science - Biotechnology
University of Houston- Clear Lake
Bachelors of Pharmacy -
Jawaharlal Nehru Technological University
Similar Profiles
Camille GibbonsCamille Gibbons
NA at MD Anderson Cancer Center (Clinical Cancer Lab)NA at MD Anderson Cancer Center (Clinical Cancer Lab)
Cynthia WilliamsCynthia Williams
Clinical Nurse, Outpatient at University of Texas MD Anderson Cancer CenterClinical Nurse, Outpatient at University of Texas MD Anderson Cancer Center
Ruby DoRuby Do
Certified Medical Assistant at MD Anderson Cancer Center HospitalCertified Medical Assistant at MD Anderson Cancer Center Hospital
Rasean SpencerRasean Spencer
Charge Nurse at MD Anderson Cancer CenterCharge Nurse at MD Anderson Cancer Center
James Hester JrJames Hester Jr
Student Assistant Mentor at Texas A&m University - KingsvilleStudent Assistant Mentor at Texas A&m University - Kingsville