Seema Chaitanya Niddapu

• Mammalian, BEVS Insect Cell culture and protein expression at Small scale (96 deep well/24 well plates), Midscale (Tubespins, flasks) and Large scale (Shake flasks, Wave bag bioreactors)
• Mammalian transient transfections and protein expression in cell lines: HEK 293, CHO
• BEVS infections viral amplifications and protein Expression in insect cell lines: SF9, Tni, Hi5.
• Supported to develop the Automation of High-throughput mammalian transfections.
• Developed and optimized the midscale mammalian expression workflow.
• Supported the optimization of new cell line Hi5 for production workflow by adapting adherent cells to suspension culture and optimization of media.
• Handled Automated High-throughput liquid handlers for transfections and protein purification.
• Semi-automated Midscale Protein Purification using tip-based column chromatography on Hamilton liquid handling systems
• Cell banking and Expanding cell lines to support research projects
• Organize and maintain the Cell bank and virus inventories.
• Supported process improvements by conducting Tech dev experiments to optimize and troubleshoot Expression/Production workflows.
• Coordinated with the team to execute multiple workflows in parallel, driving informed resource decisions for protein production and expression analysis.
• Established and refined requirements for internal workflow management tools to streamline processes with bioinformatics colleagues
• Writing protocols and reports for the workflows.
• Presentations on findings and pipeline reports in weekly department meetings.
• Instruments - Vicell BLU, Vicell XR, Hamilton Vantage (Automated transfections), Multidrop (Cell dispensing), Spin Vessel, Wavebag bioreactors, Nano drop, SDS PAGE, Hamilton Microlab STAR, Hamilton Vantage(purification)

• Culturing, Expansion of mammalian clonal cell lines (CHO, HEK, DLD1, C2C12, U2OS)
• Maintained 50+ mammalian cell lines at a time by adhering to stringent TATs of different projects
• Cell banking and production of cell stocks and managing of frozen cell stock libraries
• Cell plate generation using Automated liquid Handlers - CyBio, Tempest and Multidrop
• Optimization and Troubleshooting of Cell Line Performance
• Perform Cell count and Viability testing using Scepter, Moxi Z and Cellometer auto T4
• Providing support for high throughput screens and profiles
• Revision and generation of SOPs to capture accurate troubleshooting steps
• Maintenance of Electronic Lab notebook using Lab archives.
• Performed complex troubleshooting and assisted in leading initiatives to solve challenging scientific problems with assays or instrumentation.

Investigating the Knockdown Effects of Genetic Modifiers in Huntington's Disease iPSC derived neurons

• Stem cell culture, maintaining cell lines (iPSC, neural progenitor cells and neurons), passaging, stem cell differentiation to I-neurons, nucleofection of siRNA
• Robotic microscope imaging, Fluorescent microscopy, phenotypic data analysis - neurite length by ImageJ, Neuron survival by Matlab
• Lab duties - Updating lab databases, morphogens aliquoting, stocking, autoclaving, orders, purchases
• Performed duties in Clean room, GLP environment and documentation of the experiments, results

• Development of conditional RNAi mediated gene knockdown in Transgenic Zebrafish models
• Plasmid isolation, Cloning and Transformation into Bacterial cultures
• Identification of zebrafish transgenic lines, Drug treatment for expression, Assessment of gene knockdown, ELISA, Phenotype analysis of transgenic embryos
• Lab Management duties - Updating lab databases, inventory stocks, plasmid cloning, paramecium culture, autoclaving, orders, purchases, maintaining zebrafish facility and feeding

• Aseptic techniques, In process production QC Analytical assay testing, Protein & Polysaccharide by BCA, Lowry and Orcinol assays.
• Maintaining SOP's, GMP documents, batch production records, QC records, validation, training records.
• Log books of equipment maintenance, Maintaining Raw materials, chemicals, glassware, assay kits utility log books, Lab entry books, preparing quality control testing excel sheets, reports.