Selvaraj Ayyamperumal
Scientist
Valhalla,NY
Summary
Researcher with expertise in cell and molecular biology specifically cancer biology, proteostasis, mitochondrial biogenesis, and protein quality control.
Overview
12
12
years of professional experience
Work History
Senior Postdoctoral Researcher
New York Medical College
Valhalla, NY
10.2024 - Current
- We are investigating the carcinogen-induced lung tumorigenesis. Hexavalent chromium (Cr(VI)) reduces the protein level of the DNA polymerase δ subunit p12 in lung epithelial and lung cancer cells by promoting its degradation through the RNF8- and CRL4Cdt2-dependent ubiquitin–proteasome pathway. This loss of p12 shifts Polδ from its tetrameric Polδ4 form to Polδ3, leading to increased DNA damage, stronger activation of the DNA damage response, and reduced cell proliferation and survival after Cr(VI) exposure. These findings show that Polδ4 helps maintain genome stability during Cr(VI)-induced stress and that p12 loss may contribute to chromium-related lung carcinogenesis. We are also studying BaP, NNK and Urethane-induced lung carcinogenesis.
- Performed biological experiments to identify the molecular mechanisms underlying carcinogen-induced lung tumorigenesis.
- Experienced skills: Cell culture techniques, in vivo lung tumor induction in mice, SDS-PAGE western blotting, Fluorescence microscopy, Confocal microscopy, Gene cloning, Agarose gel electrophoresis, CRISPR-Cas knockdown, soft agar assay, RT-PCR, Amplicon sequencing, Mutational analysis, single cell genomics, Seahorse mitostress analysis etc.
Postdoctoral Researcher
IMOl Polish Academy of Science (PAN)
Warsaw
12.2021 - 09.2024
- Mitochondrial dysfunction delays mitochondrial protein import, causing accumulation of non-imported mitochondrial proteins in the cytosol and challenging proteostasis. Cells respond by increasing proteasome activity and molecular chaperones in yeast and C. elegans. In humans, Immunoproteosome is activated upon mitochondrial dysfunction. Still, several consequence mechanisms were not completely understood between mitochondria and UPS.
- Performed biological experiments to identify the molecular mechanisms underlying mitochondria and the Ubiquitin Proteasome System.
- Experienced skills: Cell culture techniques, SDS-PAGE western blotting, Fluorescence microscopy, Confocal microscopy, Gene cloning, Agarose gel electrophoresis, Immunopurification pull-down assays, Mass spec sample preparation and data analysis, AlphaFold protein complex prediction and data analysis.
Doctoral Researcher
JSS College of Pharmacy Ooty (JSS Academy of Higher Education & Research)
Mysore
10.2015 - 05.2021
- Designed and performed experiments to interpret the role of deltacarbolines as anticancer agents targeting to Topoisomerase IIa.
- Computational screening of drug-like compounds targeting Topoisomerase IIa and further tested for in vitro (cytotoxicity, inhibition assays) and in vivo (toxicity and anti-tumor activity).
- Worked on multiple collaborative projects.
- Experienced skills: Computational design of drug-like compounds, screening through ADMET properties, Molecular docking, Molecular simulation, in vitro drug screening, cytotoxicity assays, inhibition assays, in vivo anti-tumor activity.
ICMR Senior Research Fellow
JSS College of Pharmacy Ooty (JSS Academy of Higher Education & Research)
Mysore
06.2019 - 10.2021
- Designed and performed experiments to interpret the role of delta carbolines as anticancer agents.
- In vivo toxicity and anti-tumor activity of delta carboline derivatives for cancer chemotherapy.
DBT Senior Research Fellow
JSS College of Pharmacy Ooty (JSS Academy of Higher Education & Research)
Mysore
12.2016 - 12.2017
- Designed and performed experiments to interpret the role of several carbolines derivatives as anticancer agents.
- Computational screening of drug-like compounds targeting Topoisomerase IIa and further tested for in vitro (cytotoxicity, inhibition assays) and in vivo (toxicity and anti-tumor activity).
DBT Junior Research Fellow
JSS College of Pharmacy Ooty (JSS Academy of Higher Education & Research)
Mysore
12.2014 - 12.2016
- Designed and performed experiments to interpret the role of carbolines as anticancer agents targeting via Topoisomerase IIa.
- Computational screening of drug-like compounds targeting several cancer targets.
Project intern
Madras University
Chennai
08.2013 - 11.2014
- M.Tech Research project entitled "Isolation and Characterization of Anti-Diabetic Compounds from Plants of North East Region of India" under the guidance of Prof.D.Velmurugan, Professor & Head, Centre of Advanced Study In Crystallography and Biophysics, University of Madras, Chennai-600025.
- Worked on crystallization and structure determination of PLA2 and GST1 enzymes.
Education
Doctor of Philosophy - Life Sciences
JSS Academy of Higher Education & Research
01.2021
Master of Technology - Biotechnology
SRM University
01.2014
Master of Science - Plant Biology and Biotechnology
Bharathiyar University
01.2011
Bachelor of Science - Plant Biology and Biotechnology
Bharathiyar University
Languages
English
Tamil
Scientific Publications
- The p12 Subunit Choreographs the Regulation and Functions of Two Forms of DNA Polymerase δ in Mammalian Cells., Xu, D.; Ayyamperumal, S.; Zhang, S.; Chen, J.; Lee, E.Y.C.; Lee, M.Y.W.T., Genes, 2025, 16, 188
- MIA40 modulates cell death induced by Apoptosis-inducing factor 1., Ben Hur Marins Mussulini, Klaudia K. Maruszczak, Selvaraj Ayyamperumal, Piotr Draczkowski, Artur Wnorowski, Mayra A. Borrero-Landazabal, Michal Wasilewski, Agnieszka Chacinska, EMBO Reports, 2024, 1835-1862
- Defective mitochondrial import as a challenge for cellular protein homeostasis., Klaudia K. Maruszczak, Selvaraj Ayyamperumal, Agnieszka Chacinska, FEBS letters, 2023, 597, 1555–1568
- Virtual high throughput screening of natural peptides against ErbB1 and ErbB2 to identify potential inhibitors for cancer chemotherapy., Sunil Kumar Patnaik, Selvaraj Ayyamperumal, Dhananjay Jade, Nagarjuna Palathoti, Krishna Swaroop Akey, Srikanth Jupudi, Michael A. Harrison, Sreenivasan Ponnambalam, Nanjan MJ & Chandrasekar MJN, Journal of Biomolecular Structure and Dynamics, 2023, 10.1080/07391102.2023.2226744
- Preliminary hypothetical assessment and in silico molecular docking of statin to VEGFR2 and VEGFR3 protein complex associated with angiogenesis and lymphangiogenesis in diabetic wounds., Divya Pamu, Selvaraj Ayyamperumal, Munikumar Manne, Vyshnavi Tallapaneni, Chandrasekar Joghee Nanjan Moola, Veera Venkata Satyanarayana Reddy Karri, J Appl Pharm Sci, 2023, 13, Suppl 1, 009–020
- Virtual High Throughput Screening: Potential Inhibitors for the Mycobacterial α subunit of Tryptophan Synthase., Surender Mohan, Dhananjay Jade, Selvaraj Ayyamperumal, Chandrasekar MJN, Sonal Gupta, MJ Nanjan., Molecular Simulation, 2021
- Potential inhibitors for FKBP51: An in-silico study using virtual screening, molecular docking, and molecular dynamics simulation., Sagar barge, Dhananjay Jade, Selvaraj Ayyamperumal, Prasenjit Manna, Jagat Borah, Chandrasekar Moola Joghee Nanjan, Moola Joghee Nanjan, Narayan Chandra Talukdar., Journal of Biomolecular Structure and Dynamics, 2021
- Plant based natural products as quorum sensing inhibitors in E.coli: a critical review., Cardiliya AP, Selvaraj A, Nanjan MJ, Chandrasekar MJN., Mini Reviews in Medicinal Chemistry, 2021
- In silico screening of FDA approved drugs against ACE2 receptor: potential therapeutics to inhibit the entry of SARS-CoV-2 to human cells., Selvaraj Ayyamperumal, Dhananjay Jade, Vyshnavi Tallapaneni, Chandrasekar MJN, Nanjan MJ., Journal of Biomolecular Structure and Dynamics, 2021, http://dx.doi.org/10.1080/07391102.2021.1960892
- Virtual High Throughput Screening: Potential Inhibitors for SARS-CoV-2 PL PRO and 3CL PRO Proteases., Dhananjay Jade, Selvaraj Ayyamperumal, Vyshnavi Tallapaneni, Chandrasekar Moola Joghee Nanjan, Sagar Barge, Surender Mohan, Moola Joghee Nanjan., European Journal of Pharmacology, 2021, 901, 174082
- Distinct Modulation of Wild-Type and Selective Gene Mutated Vitamin D Receptor by Essential Polyunsaturated Fatty Acids., Hari Balaji, A. Selvaraj, Niladri Saha, P. Shyam Sundar, S. Jubie, S. Dhivya and Suresh K. Mohankumar., Mini Reviews in Medicinal Chemistry, 2021, 10.2174/1389557521666210104170408
- Novel δ-Carboline Inhibitors for α-Topoisomerase II: Potential Anti-Cancer Agents., Selvaraj Ayyamperumal, Dhananjay DJ, Surender Mohan, Kaviarasan L, Basappa S, Jubie Selvaraj, Nanjan Moola Joghee, Chandrasekar MJN., Bioinformation, 2021, 17, 1, 249-265
- Targeting a conserved pocket (n-octyl-β-D–glucoside) on the dengue virus envelope protein by small bioactive molecule inhibitors., P Naresh, A Selvaraj, P Shyam Sundar, S Murugesan, S Sathianarayananan, Krishnan Namboori PK, S Jubie., Journal of Biomolecular Structure and Dynamics, 2020
- Design and Synthesis of a Novel Gabapentin-Phosphotidylcholine Conjugate for Targeting to Phospholipase A2 Enzyme through Nanostructured Lipid Carrier., PB Anjali, N Jawahar, S Jubie, Neetu Yadav, A Selvaraj, S Selvamuthukumar., Current Computer-aided Drug Design, 2020, 2020-12-24
- Structural and functional analysis of Chitinase-IV of Brassica juncea: Molecular modelling and Dynamic simulation study., Selvaraj Ayyamperumal, Dhananjay Jade, Surender Mohan, Nanjan MJ, Chandrasekar MJN., Journal of Biomolecular Structure and Dynamics, 2020
- Positive allosteric activation of glial EAAT-2 transporter protein: A novel strategy for Alzheimer's disease., Manisha Reddy, Selvaraj A, Jubie Selvaraj, Chandrasekar MJN, Nanjan Moola Joghee, James Chelliah, Justin Antony., Medical Hypothesis, 2020, September, 109794
- A strategy to treat the breast cancer through inhibiting the overexpression of protein estrogen via schiff base fused coumarin: an in-silico based synthetic approach., Pradeepa T, Selvaraj A, Jubie Selvaraj., International Journal of Pharmaceutical Sciences and Research, 2020, 11, 9, 4349-58
- Early stage repurposing of benzimidazole scaffolds towards breast cancer through in-silico tools., Jubie S, Selvaraj A, Neethu Y, Parvesh B, Puru K, Shreyan S, Chandrasekar MJN., Journal of Pharmaceutical Sciences and Research, 2018, 10, 10, 2419-2423
- Novel HDAC8 inhibitors: A multi-computational approach., M. Manal, K. Manish, D. Sanal, A. Selvaraj, V. Devadasan & M. J. N. Chandrasekar., SAR and QSAR in Environmental Research, 2017, September, 28, 9, 707-733
- Identification of human NMPrtase inhibitors Adenocarpus Manii; An in-silico approach., Blaise K. Nganou, pierre Tane, Alex Nchiozem, Jubie Selvaraj, Selvaraj A, Chandrasekar MJN., International Journal of Pharmaceutical Sciences and Research, 2017, 9, 2, 95-99
Scientific Training
- Artificial Intelligence (AI) and Human Subject Protections, New York Medical College, 2025-01-01
- Confocal microscopy and fluorescence microscope training, Centre of New Technologies, University of Warsaw, 2022-07-04
- Isolation of primary mammary organoids and 3D-culture, whole-mount analysis, Immunohistochemistry, Phenotypic Heterogeneity as a Driver of Cancer Progression, Indian Institute of Science, Bengaluru, 2019-01-07
- Gene editing using CRISPR-CAS, JSS College of Pharmacy, Ooty, 2018-12-13
- Handling infectious pathogens in BSL-3 Facility, Department of Biotechnology, Jawaharlal Nehru University, New Delhi, 2018-04-17
- Cell Cycle Analysis and Apoptosis Assay, Department of Biotechnology, Bhupat, and Jyoti Mehta School of Biosciences, IIT Madras, Chennai, 2017-05-25
- Bioinformatics Workshop on Genomics, Proteomics, Drug Design & High Performance Computing, Supercomputing Facility for Bioinformatics & Computational Biology (SCFBio), IIT Delhi, 2016-08-01
- Cell Culture Techniques, Cell Proliferation Assays, and Gene Expression Analysis by RT-PCR, JSS College of Pharmacy, Ooty, 2016-07-14
Conference Presentations
- Metal Toxicity and Carcinogenesis, Stony Brook Cancer Centre, New York, United States, 2025-01-01, Suppression of the p12 subunit of DNA polymerase δ by hexavalent chromium in lung epithelial and lung cancer cells and its implications in lung carcinogenesis.
- LW 10th Mitochondrion Meeting, Nencki Institute of Experimental Biology, Warsaw, Poland, 2023-06-20, Deubiquitinase UCH-L1 and its link with mitochondrial dysfunction.
- Cologne Spring Meeting, Cologne, Germany, 2023-03-07, Ubiquitin carboxyl-terminal hydrolase L1 and its link with mitochondrial dysfunction.
- EMBO Conference, Srebreno-Dubrovnik, Croatia, 2023-05-21, Deubiquitinase UCH-L1 and its role with mitochondrial dysfunction.
- GRC Conference, Renaissance Tuscany Il Ciocco, Lucca (Barga), LU, Italy, 2023-02-05, Deubiquitinase UCH-L1 and its link with mitochondrial dysfunction.
- One Health India Conference, Department of Biotechnology, Ministry of Science and Technology, Government of India, New Delhi, 2019-02-18, DNA Topoisomerase inhibitors for pathogenic cancer.
- 3rd PAN IIT BIOTECH MEET 2019, IIT Madras, Chennai, 2019-01-31, Novel carboline/carbazole derivatives as anti-cancer agents via induction of apoptosis and cell cycle arrest.
- Cellular Heterogeneity in Cancer Progression, IISc, Bengaluru, 2019-01-05, Novel carboline derivatives as anti-tumour agents via induction of apoptosis.
- EMBL Conference: Cancer Genomics, EMBL Heidelberg, Germany, 2017-11-05, Cancer chemotherapeutic evaluation of some carboline derivatives towards drug design and development.
- World Congress on Pharmaceutical Sciences, Goa, 2017-10-05, Intracellular targeting of Elastin Like Polypeptide-Drug conjugates to solid tumors by phase-structure transition.
- International workshop on Malaria Parasite Biology, ICGEB, New Delhi, 2017-11-29, Topoisomerase; A Novel Anti-Viral Target.
Awards Scholarships
- Researcher Travel Award from Stony Brook Cancer Centre.
- Received Department of Biotechnology, Govt. of India, Junior/Senior Research Fellowships.
- Received Indian Council of Medical Research, Govt. of India, Senior Research Fellowship.
- Received Bioinformatic Studentship Scholarship (From University of Madras).
- Received the award of Outstanding Research Scholars Coordinator.
Personal Information
Title: Senior Postdoctoral Researcher
Timeline
Senior Postdoctoral Researcher
New York Medical College
10.2024 - Current
Postdoctoral Researcher
IMOl Polish Academy of Science (PAN)
12.2021 - 09.2024
ICMR Senior Research Fellow
JSS College of Pharmacy Ooty (JSS Academy of Higher Education & Research)
06.2019 - 10.2021
DBT Senior Research Fellow
JSS College of Pharmacy Ooty (JSS Academy of Higher Education & Research)
12.2016 - 12.2017
Doctoral Researcher
JSS College of Pharmacy Ooty (JSS Academy of Higher Education & Research)
10.2015 - 05.2021
DBT Junior Research Fellow
JSS College of Pharmacy Ooty (JSS Academy of Higher Education & Research)
12.2014 - 12.2016
Project intern
Madras University
08.2013 - 11.2014
Doctor of Philosophy - Life Sciences
JSS Academy of Higher Education & Research
Master of Technology - Biotechnology
SRM University
Master of Science - Plant Biology and Biotechnology
Bharathiyar University
Bachelor of Science - Plant Biology and Biotechnology
Bharathiyar University