Summary
Overview
Work History
Education
Skills
Presentations
Authored Research
Professional Affiliations
References
Timeline
Generic

Shaela Nestor

Silver Spring,MD

Summary

Proficient in microbiology and laboratory quality control, with experience in research, analysis, and data interpretation. Strong expertise in the scientific method, adherence to safety protocols, and the ability to manage complex projects.

Overview

2
2
years of professional experience

Work History

Microbiologist, Laboratory Quality Control

Certis Biologicals
Columbia, MD
09.2024 - Current

40/Week

$60,000/Year

  • Conducted quality assurance assessments on company products to ensure adherence to standards
  • Updated and maintained records with accurate and current data as required
  • Prepared and delivered monthly reports outlining project progress and key performance indicators
  • Evaluated the compatibility of company products with those of partner organizations
  • Supported fermentation processes in the production of company products

Microbiology Laboratory Technician

Cold Regions Research and Engineering Laboratory
Hanover, NH
07.2023 - 07.2024

40 hours/Week

$46,000/Year

  • Researched and proposed new methodology for psychrophilic bacterial extraction and characterization from arctic soil samples
  • Contributed 20-30 bacterial isolates to the ICE-COLD (Innovative, Collaborative, Exploratory Cold regions Organismal Library for Discovery) library
  • Revived, characterized, and conducted various experimentation on 50 bacterial isolates for the DARPA-ICE (Defense Advanced Research Projects Agency- Ice Control of cold Environments) program
  • Formulate quarterly reports of project progress
  • Performed aseptic decontamination of ancient permafrost cores
  • Engage in oral presentations of data collection, efficiency, and experimental alterations monthly
  • Actively engage in safety briefs and training as required

Research Intern

Cold Regions Research and Engineering Laboratory
Hanover, NH
06.2023 - 07.2023
  • Modernized the ICE-COLD 160+ microbial isolate library and updated it with pertinent data
  • Designed and conducted a freeze-thaw cyling experiment on arctic soil samples to extract and charcterize microbes with antifreeze proteins
  • Characterized 12 bacterial isolates that were isolated from the freeze-thaw cycling study
  • Proposed and assessed new protocols for microbial isolation

Laboratory Assistant

College of Coastal Georgia
Brunswick, GA
09.2022 - 05.2023
  • Performed routine laboratory maintenance on various equipment
  • Monitored inventory of laboratory chemicals and materials
  • Structured and prepared 3 labs for current CCGA students
  • Aliquoted and prepared reagents needed for experimentation
  • Monitored lab safety regulations and ensured compliance with safety procedures

Education

Master of Science - Biodefense

George Mason University
Fairfax, VA
05-2026

Bachelor of Science - Biochemistry

College of Coastal Georgia
Brunswick, GA
05-2023

Skills

  • Data visualization through R and Python
  • Microsoft Office
  • DNA extraction and quantification
  • High-Performance Liquid Chromatography
  • Bacterial and phage culturing
  • Environmental sampling
  • Polymerase Chain Reaction
  • Plasmid extraction
  • Gel electrophoresis
  • Bacterial transformation
  • Reagent preparation
  • Microscopy
  • Omnilog Utilization
  • Flow cytometry
  • Nuclear Magnetic Resonance
  • Gas Chromatography-Mass Spectrometry
  • Fourier-transform infrared spectroscopy
  • Inductively Coupled Plasma Atomic Emission Spectroscopy
  • Bomb calorimeter
  • Quality Assurance

Presentations

Elizabeth J. Corriveau, Shaela M. Nestor, Lina J. Bird, Daniel W. Tague, Emily Asenath-Smith, Robyn A. Barbato. “Exploring Arctic Microbes through Freeze-Thaw Culturing.” Poster session presented at: Tri-Service Microbiome Consortium; 2023 September 26-27; Arlington, VA.

Shaela M. Nestor, Elizabeth J. Corriveau, Lindsay I. Gaimaro, Robyn A. Barbato. "Ice Breakers: Using freeze-thaw cycling to culture bacteria from terrestrial ice wedges” Poster session presented at: CRREL Student Science Symposium; 2023 August 10; Hanover, NH.

Shaela Nestor, Kendra Paulson. “Epigenetics of P5CS in Zea may in response to salinity stress." Poster session presented at: Symposium on Undergraduate Research and Collaborative Exploration; 2023 May 1; Brunswick, GA.

Shaela Nestor. "Phage Mopey Gene 13" Poster session presented at: Symposium on Undergraduate Research and Collaborative Exploration; 2023 May 1; Brunswick, GA.

Authored Research

Co-author on a Microbiology Resource Announcement (MRA) currently under review for the American Society for Microbiology Journal

This announcement aims to share the ICE COLD culture collection resource (over 100 specimens) with the scientific community as these microorganisms possess potentially unique properties that could be valuable for biotechnological applications.

Professional Affiliations

Member of the American Society for Microbiology

March 2024 - Present

References

References available upon request.

Timeline

Microbiologist, Laboratory Quality Control

Certis Biologicals
09.2024 - Current

Microbiology Laboratory Technician

Cold Regions Research and Engineering Laboratory
07.2023 - 07.2024

Research Intern

Cold Regions Research and Engineering Laboratory
06.2023 - 07.2023

Laboratory Assistant

College of Coastal Georgia
09.2022 - 05.2023

Master of Science - Biodefense

George Mason University

Bachelor of Science - Biochemistry

College of Coastal Georgia
Shaela Nestor