Summary
Overview
Work History
Education
Skills
Computational Skills
Laboratory Skills
Publications
References
Accomplishments
Languages
Work Availability
Timeline
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Shailesh Karre

Shailesh Karre

Hayward,CA

Summary

Dedicated and highly motivated laboratory scientist well-versed in plant pathology, plant-microbe interactions, and plant physiology-related studies. Excellent multitasker with good communication and problem-solving abilities. Skilled at setting up new plant screening facilities for bio-fertility programs.

Overview

15
15
years of professional experience

Work History

Research Scientist

Pattern Ag
Emeryville, CA
06.2022 - 01.2024
  • Worked as lead scientist for Plant Biology, mentored/managed four junior scientists, and delegated tasks to complete plant biology-related experiments.
  • Designed and maintained a semi-controlled plant growing facility (low-cost and effective grow tents), to perform studies in row crops such as corn and soybean related to biofertility.
  • Developed and implemented new screening protocols to identify crop-beneficial Phosphate solubilizing bacteria (PSBs).
  • Identified potential problems and developed solutions related to research projects.
  • Recorded and reported test results, and produced graphical representations.
  • Provided technical support during presentations of project progress at internal team meetings or with external teams.
  • Collaborated with other researchers from different departments within the organization to develop more effective strategies for conducting experiments.

Postdoctoral Research Fellow

North Carolina State University
01.2020 - 01.2021
  • Identifying endophytic fungi from wheat, maize, and switchgrass and studying their role in disease resistance against wheat and maize fungal pathogens
  • Developed detached leaf assays in Maize and Wheat.

Postdoctoral Research Fellow

North Carolina State University
01.2017 - 01.2021
  • Studied the role of candidate genes (E3 ligase proteins) identified using GWAS for maize hypersensitive response
  • Characterized two maize E3 ubiquitin ligase genes involved in regulating maize hypersensitive response.

Ph.D. in Plant Sciences

McGill University
01.2012 - 01.2016
  • Identification of disease resistance in barley for fusarium head blight based on metabolo-transcriptomics approach
  • Characterization of two candidate genes HvCERK1 and HvWRKY23, and their role in conferring fusarium head blight resistance.

Research Scholar

ICRISAT
01.2011 - 01.2012
  • My research involved molecular and physiological characterization of DREB transgenics in peanuts, and introgression of DREB gene to other peanut genotypes through back crossbreeding.

Graduate student

University of Agricultural Sciences
01.2009 - 01.2010
  • Development of peanut transgenics harboring three regulatory genes such as PDH45, GBF1, and EPSPS using multi-site gateway cloning technology
  • The resultant transgenics exhibited oxidative stress and dehydration stress tolerance.

Education

Ph.D. - Plant Sciences

McGill University

MSc (Agriculture) - Crop Physiology

University of Agricultural Sciences

BSc - Agricultural Sciences

University of Agricultural Sciences

Skills

  • Research Planning, Experiment Design, and execution
  • Experiment Protocol Development
  • Data Analysis and Interpretation of Findings, Report Preparation
  • Laboratory setup and management skills
  • Technical Troubleshooting
  • Analytical Thinking
  • Research and Publication
  • Leading Focus Groups
  • Deadline Adherence
  • Good communication skills
  • Self-Driven and Motivated

Computational Skills

  • Abinitio Software’s like SWISS-MODEL, Phire2, PyMOL
  • MzMine2, METLIN for metabolomic data analysis
  • GraphPad Prism for making graphs and statistical analysis of data
  • SnapGene for cloning simulation
  • Geneious for basic molecular biology
  • Microsoft office

Laboratory Skills

  • In-planta screening assays for biofertility
  • Microphenotron assay
  • Cloning (traditional, gateway, and Golden Gate cloning)
  • QPCR, RT-qPCR, and PCR
  • Bacterial transformation (both E. Coli and Agrobacterium tumefaciens)
  • Protein expression, extraction, SDS-PAGE gel electrophoresis, and western blot
  • Co-Immunoprecipitation assay and Bimolecular Fluorescence assay
  • Development of transgenics (tobacco, peanuts, and arabidopsis)
  • Virus-induced gene silencing (maize, wheat, barley, and potato)
  • CDNA library preparation and generation of expressed sequence tags

Publications

https://scholar.google.com/citations?user=VIUsnW8AAAAJ&hl=en

References

References available upon request

Accomplishments

  • Received 5 fellowships during Ph.D. due to excellent academic and research track record
  • Gold medal awardee in bachelor for being topper of the batch

Languages

English
Full Professional
Hindi
Professional
Telugu
Native/ Bilingual

Work Availability

monday
tuesday
wednesday
thursday
friday
saturday
sunday
morning
afternoon
evening
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Timeline

Research Scientist

Pattern Ag
06.2022 - 01.2024

Postdoctoral Research Fellow

North Carolina State University
01.2020 - 01.2021

Postdoctoral Research Fellow

North Carolina State University
01.2017 - 01.2021

Ph.D. in Plant Sciences

McGill University
01.2012 - 01.2016

Research Scholar

ICRISAT
01.2011 - 01.2012

Graduate student

University of Agricultural Sciences
01.2009 - 01.2010

Ph.D. - Plant Sciences

McGill University

MSc (Agriculture) - Crop Physiology

University of Agricultural Sciences

BSc - Agricultural Sciences

University of Agricultural Sciences
Shailesh Karre