Open To Work
Shankar Pandey
New Haven,USA
Summary
Postdoctoral researcher with a Ph.D. in analytical chemistry, specializing in protein engineering and bioconjugation, Noncanonical amino acid incorporation & expression, Recombinant protein production, Antibody structure prediction, Disulfide engineering, and Biochemical validations. Demonstrated ability to improve conjugation yield by threefold and reduce purification processing time by 30%, enhancing research efficiency and data quality. Author of multiple SOPs to support reproducibility and contribute to peer-reviewed publications.
Overview
17
17
years of professional experience
Work History
Postdoctoral Researcher
Yale University
New Haven, Connecticut
08.2023 - Current
- Improved conjugation yield and recombinant protein yield by threefold through systematic optimization of transient transfection parameters.
- Established stable mammalian cell lines with consistent expression.
- Reduced purification processing time by 30% by implementing streamlined chromatography workflows (Affinity, SEC).
- Developed and validated 3+ reproducible cell-based assays supporting protein and antibody characterization.
- Designed the plasmid, performed molecular cloning, and expressed and purified the recombinant protein.
- Conducted advanced research in molecular biology techniques and methodologies.
- Developed new experimental protocols to enhance research efficiency and accuracy.
- Authored 4+ SOPs to enhance experimental reproducibility.
- Contributed to publications in peer-reviewed journals by writing manuscripts, preparing figures, and editing text.
- Mentored graduate students in research techniques and laboratory protocols.
- Prepared samples for electron microscopy imaging studies.
- Adhered to all regulatory standards and university regulations for reporting.
- Synthesized different bioconjugation chemistry to increase the conjugation yields.
- Utilized advanced statistical and computational techniques for data analysis.
Research & Teaching Assistant
Kent State University
Kent, Ohio
07.2017 - 05.2023
- Developed 8+ bioconjugation chemistry protocols and optimized conjugation yields, enhancing experimental outcomes.
- Led independent research like host-guest chemistry, peptide-peptide interaction, and de-novo screening of drugs, resulting in 6+ first authors and nine contributing authors in peer-reviewed journals.
- Developed a novel technique to investigate the inhibitory effect of different natural products on nucleation of Aβ-oligomer.
- Developed a novel method to screen the small molecules for the mechano-pharmaceutical method.
- Designed the plasmids and purified the recombinant protein for screening of drugs.
- Managed cross-functional research projects with national and international collaborators, facilitating collaboration and advancing research objectives.
- Mentored 4+ graduate and undergraduate students, fostering skill development and contributing to overall lab efficiency.
- Participated actively in regular meetings with fellow researchers to discuss project updates, challenges faced, and lessons learned during ongoing activities.
- Organized research materials, maintaining a well-ordered workspace conducive to productivity.
Lecturer
Oxford College of Science
Chitwan, Nepal
06.2015 - 05.2017
- Developed and delivered engaging lectures on Physical, Organic, Analytical and Inorganic chemistry.
- Implemented innovative teaching techniques to foster critical thinking skills.
- Created and designed quizzes, tests, and projects to effectively assess and enhance student knowledge.
- Evaluated student progress through regular assessments, providing detailed feedback for improvement and growth.
- Mentored students in research projects, guiding them through complex scientific inquiries to develop their research skills.
- Supervised chemistry laboratory safety by implementing strict guidelines and ensuring proper equipment usage.
Lecturer
Kanjirowa National Higher Secondary College
Kathmandu, Nepal
07.2011 - 06.2015
- Designed course materials that improved student understanding and engagement.
- Cultivated critical thinking skills through assignments requiring analysis, synthesis, and evaluation of diverse information sources.
- Evaluated student progress through regular assessments, providing detailed feedback for improvement and growth.
- Collaborated with faculty and staff to create meaningful learning experiences.
- Developed strong relationships with colleagues, collaborating on curriculum development and sharing best practices in teaching strategies.
- Mentored and advised students on career pathways, enhancing their knowledge, skills, and strengths.
M. Sc. Researcher
Tribhuvan University
Kathmandu, Nepal
06.2009 - 07.2011
- Acquired expertise in organic, physical, analytical, inorganic chemistry, and research methodology to enhance experimental design.
- Performed multistep organic synthesis to modify the surface functional group of the polymer.
- Synthesized and characterized the modified and unmodified sugarcane bagasse.
- Evaluated adsorbent effectiveness on methyl red dye adsorption behavior, contributing to understanding of dye removal processes.
- Wrote a comprehensive scientific article and published the findings.
- Performed comprehensive literature reviews to inform and guide research project directions.
Education
Postdoctoral Research Associate - Protein Engineering & Bioconjugation Chemistry
Yale University
CT, USA01-2026
Ph.D. - Analytical Chemistry
Kent State University
OH, USA05-2023
Master of Science - Physical Chemistry
Tribhuvan University
Nepal07-2011
Bachelor of Science - Chemistry
Tribhuvan University
Nepal06-2009
Skills
Technical Skills:
- Bioconjugation Chemistry (CuAAS, SPAAS, NHS ester-amine, APN-thiol, Maleimide-thiol, Bromo/Iodoacetamide-thiol, & Enzymatic)
- Site-specific Conjugation of Nanobody and Nucleic Acids,
- Nonnatural Amino Acid Incorporation and Expression,
- Radioisotope and Fluorophore Labeling,
- Enzyme-linked Immunosorbent Assay (ELISA),
- Molecular Cloning & Protein Engineering,
- Recombinant Protein Expression & Purification,
- DNA & RNA isolation,
- qPCR & PCR (two-step PCR, overlap extension PCR),
- Cell Culture Experience (HUVEC, MAEC, HAEC, COS7, NIH3T3, HEK293T),
- Stable Cell Line Development (iMAEC, teloHAEC),
- Cell-based Assay Development & Validation (adhesion, migration, invasion assay),
- Vector Design and Mutagenesis,
- Bacterial Transformation and Transfections,
- Viral Transduction and Antibiotic Selection,
- DNA Origami Design and Preparation,
- Single-molecule Force Spectroscopy,
- Circular Dichroism (CD) Spectroscopy,
- Immunofluorescence Microscopy (IFM),
- UV Spectroscopy,
- Confocal Microscopy,
- Transmission Electron Microscopy (TEM),
- Nanopore & Sanger Sequencing,
- Affinity, Ion Exchange (IEX) & Size Exclusion Chromatography (SEC),
- GC, HPLC & Analytical Characterization,
- Gel Electrophoresis and Western Blot
Transferable Skills:
- Teamwork,
- Leadership,
- Time management,
- Listening,
- Critical thinking,
- Multitasking,
- Creativity,
- Troubleshooting,
- Problem-solving
Data analysis
- Microsoft Office (Word, Excel, PowerPoint),
- Programming Language (Python and R-studio),
- CorelDRAW,
- Igor,
- GraphPad Prism,
- ImageJ,
- Maya (3D animation)
Portfolio & Profile
www.linkedin.com/in/shankar-pandey-1345b3321
Google Scholar
Presentations & Publications
- Sixteen peer-reviewed scientific articles with seven first authors and nine contributing authors, one book chapter, oral presentation in Graduate research symposium (GRS), Advance materials and liquid crystal institute (AMLCI) material day, and Gordon Research Conference (GRC).
Selected Publications:
- Kun Zhou, Minhwan Chung, Shankar Pandey, Jing Cheng, John T Powell, Qi Yan, Jun Liu, Yong Xiong, Martin A Schwartz, Chenxiang Lin, “DNA nanodevice for analysis of force-activated protein extension and interactions”. Nature Nanotechnology (2025).
- Shankar Pandey, Mathias Bogetoft Danielsen, Yuan Xiang, Zhilei Zhang, Grinsun Sharma, Byeong Tak Jeon, Shixi Song, Yitong Hao, Gunan Zhang, Niels Johan Christensen, Kasper Kildegaard Sorensen, Pernille Harris, Pravin Pokhrel, Richard Cunningham, Min-Ho Kim, Yongsheng Leng, Chenguang Lou, Hanbin Mao, “De novo design of a mechano-pharmaceutical screening platform against formation of individual beta-amyloid oligomers”. Cell Reports Physical Science (2024).
- Shankar Pandey, Shankar Mandal, Mathias Bogetoft Danielsen, Asha Brown, Changpeng Hu, Niels Johan Christensen, Alina Vitaliyivna Kulakova, Shixi Song, Tom Brown, Knud J. Jensen, Jesper Wengel, Chenguang Lou, Hanbin Mao, “Chirality transmission in macromolecular domains”. Nature Communication (2022).
- Shankar Pandey, Yuan Xiang, Dirk Friedrich, Yongsheng Leng, Hanbin Mao, “Direct Measurement of Intermolecular Mechanical Force for Non-specific Interactions between Small Molecules”. Journal of Physical Chemistry Letters (2021).
- Shankar Pandey, Yuan Xiang, Dilanka V. D. Walpita Kankanamalage, Janarthanan Jayawickramarajah, Yongsheng Leng, Hanbin Mao, “Measurement of Single-Molecule Forces in Cholesterol and Cyclodextrin Host-Guest Complexes”. Journal of Physical Chemistry B (2021).
- Shankar Pandey, Yuanyuan Li, Montwan D. Young, Shankar Mandal, Laichun Lu, Jacob T. Shelley, Hanbin Mao, “Cooperative Heteroligand Interaction with G-quadruplexes shows Evidence of Allosteric Binding”. Biochemistry (2020).
- Shankar Pandey, Dilanka V.D Walpita Kankanamalage, Xiao Zhou, Changpeng Hu, Mohammed Enamul Hoque, Lyle Isaacs, Janarthanan Jayawickramarajah, Hanbin Mao, “Chaperone-Assisted Host-Guest Interactions Revealed by Single-Molecule Force Spectroscopy”. Journal of the American Chemical Society (2019).
Volunteer Experiences
- Judge, STEM EXPO 2021, Akron Public Schools' Science, Technology
- Member, Biophysical Society Chapter, Kent State University, 2019-2023
- Vice-president, Leo Club of Bharatpur, Chitwan, Nepal, 2008-2009
Accomplishments
• Graduate Research Award (2022), Kent State University, Kent, OH
• University Fellowship Recipient (2022), Kent State University, Kent, OH
• Taylor Graduate Scholarship (2021), Kent State University, Kent, OH
• University Fellowship Nominee (2020 and 2021), Kent State University, Kent, OH.
• Taylor Graduate Scholarship, Honorable Mention (2020), Kent State University, Kent, OH.
• Nepal Vidyabhusan ‘B’ (2013), Government of Nepal
• P.M. Singh gold medal and Amrit medal (2012), Tribhuvan University, Nepal
• Kriti Sanu Padak from Shess Hera Foundation (2011), Nepal
• Basant Bikram-Gyanu Shah Research Fund Award (2008), Tribhuvan University, Nepal
• University Fellowship Recipient (2022), Kent State University, Kent, OH
• Taylor Graduate Scholarship (2021), Kent State University, Kent, OH
• University Fellowship Nominee (2020 and 2021), Kent State University, Kent, OH.
• Taylor Graduate Scholarship, Honorable Mention (2020), Kent State University, Kent, OH.
• Nepal Vidyabhusan ‘B’ (2013), Government of Nepal
• P.M. Singh gold medal and Amrit medal (2012), Tribhuvan University, Nepal
• Kriti Sanu Padak from Shess Hera Foundation (2011), Nepal
• Basant Bikram-Gyanu Shah Research Fund Award (2008), Tribhuvan University, Nepal
Work Preference
Job Search Status
Open to workWork Type
Full TimePart TimeLocation Preference
On-SiteHybridSalary Range
$78000/yr - $120000/yrTimeline
Postdoctoral Researcher
Yale University
08.2023 - Current
Research & Teaching Assistant
Kent State University
07.2017 - 05.2023
Lecturer
Oxford College of Science
06.2015 - 05.2017
Lecturer
Kanjirowa National Higher Secondary College
07.2011 - 06.2015
M. Sc. Researcher
Tribhuvan University
06.2009 - 07.2011
Postdoctoral Research Associate - Protein Engineering & Bioconjugation Chemistry
Yale University
Ph.D. - Analytical Chemistry
Kent State University
Master of Science - Physical Chemistry
Tribhuvan University
Bachelor of Science - Chemistry
Tribhuvan University