Shannon Hott
Frederick,CO
Summary
Dedicated and experienced laboratory researcher skilled in designing and executing experiments that advance project objectives. Expertise in multidisciplinary technical research and analytical skills, management of laboratory, operations, and mouse colony, contributing significantly to research outcomes and team collaboration.
Overview
16
16
years of professional experience
Work History
Senior Laboratory Researcher
National Jewish Health
Denver, Colorado
04.2018 - Current
- Designed experiments to test hypotheses related to the project's objectives while adhering to ethical standards of research practice.
- Analyzed data using statistical software to identify trends and patterns.
- Collaborated with multidisciplinary teams on research project development.
- Troubleshot experimental issues and adjusted protocols to achieve successful outcomes, contributing to project continuity.
- Maintained mouse colony including census and budget, breeding strategies, genotyping, and communication with veterinarian staff
- Ensured optimal performance and reliability of laboratory equipment through regular maintenance and calibration.
- Documented research findings in compliance with regulatory standards and protocols.
- Trained new staff on laboratory procedures and safety guidelines, providing technical guidance and mentorship to junior researchers to enhance team competency.
- Presented findings at scientific conferences and meetings with colleagues.
Research Lab
University of Northern Colorado
08.2017 - 12.2017
- Studied cell adhesion components that contribute to choroid fissure closure in Zebrafish
- Zebrafish colony and breeding, Conducted polymerase chain reaction (PCR) experiments to analyze genetic material., maintenance of lab reagents and materials
- Collaborated with faculty to analyze research findings and develop presentations.
- Organized and maintained research databases for easy access and retrieval of information.
- Participated in team meetings to discuss progress and share insights on projects.
- Presented findings for faculty, facilitating discussions on research progress.
Laboratory Intern
University of Northern Colorado
08.2016 - 08.2017
- Cell culture and treatments, qPCR, primer design, flow cytometry, microscopy, maintenance of lab equipment, reagents, and materials
- Conducted experiments following established protocols in a university laboratory setting.
- Documented experimental procedures and results for ongoing research initiatives.
- Maintained cleanliness and safety standards within the laboratory environment at the university.
- Developed presentations summarizing findings from laboratory experiments.
Safety Consultant
Weld County Welding
01.2016 - 01.2017
- Conducted regular inspections to identify potential hazards in the workplace.
- Assisted in upholding safety protocols and ensuring compliance with regulations to mitigate risks.
- Monitored safety equipment and ensured proper usage among employees.
- Reported safety violations in the field and educated employees on safety practices to promote a culture of safety.
- Documented safety incidents and contributed to investigations to implement corrective actions.
Receptionist/ Front Office
Carden Academy
01.2011 - 01.2013
- Welcomed visitors at reception, ensuring a friendly atmosphere. and provided them with assistance.
- Answered multi-line phone system and directed calls efficiently.
- Coordinated meetings, including room setup and necessary materials preparation.
- Maintained a neat reception area by organizing materials and tidying up furniture.
- Processed incoming and outgoing mail with attention to detail.
- Maintained office supplies inventory and ordered replacements.
- Updated and recorded customer or client information to maintain accounts.
Education
B.S. - Molecular Biology
University of Northern Colorado
Greeley, CO12-2017
Majored in Biology -
Regis University
Denver, CO05-2006
Skills
- Flow cytometry
- Mouse bronchoscopy
- Mouse colony breeding, overseeing cost and budget of colony, genotyping
- Cell culture
- Maintenance of cell lines, incubators, medias
- Maintainence of laboratory equipment, supplies, reagents
- Intratracheal instillations, Intraperitoneal, subQ, and IV injections
- Organ harvest
- Microsoft Office, Excel, Powerpoint, Prism, Flowjo, MSD software
- IF/histology, microscopy
- ELISA
- Mesoscale
- Fluorescent antibody staining
- RNA isolation and qPCR
- Live Cell Fluorescent Microscopy
- BSL2 and BSL3 experience
- Clinical processing and organization of Covid samples
- Murine bone marrow harvests and transplant surgery
- Western Blot
- Processing of Human bronchoalveolar lavage
- RNAscope Histology
- Chimeric mouse generation
- Interviewing and training new hires
- Collaboration on multi-lab projects
Timeline
Senior Laboratory Researcher
National Jewish Health
04.2018 - Current
Research Lab
University of Northern Colorado
08.2017 - 12.2017
Laboratory Intern
University of Northern Colorado
08.2016 - 08.2017
Safety Consultant
Weld County Welding
01.2016 - 01.2017
Receptionist/ Front Office
Carden Academy
01.2011 - 01.2013
B.S. - Molecular Biology
University of Northern Colorado
Majored in Biology -
Regis University