SHARMISTA PRATTIPATI
Greensboro,NC
Summary
Meticulous and analytical Researcher with hands-on experience in cell based toxicity assays, microscopic and analytical techniques. Adaptive team player with in-depth knowledge of experimental design, data collection and problem-solving.
Overview
13
13
years of professional experience
Work History
Adjunct Chemistry Instructor
North Carolina Agricultural And Technical State University
08.2021 - Current
- Engaged students and promoted intellectual curiosity through hands-on experiments and PowerPoint presentations to provide positive attitude towards scientific processes.
- Instructed students in lab by teaching safety protocols for proper use of chemicals and safe disposal of hazardous materials.
- Participated in department meetings to provide input to colleagues about student achievement and improvement.
- Maintained current knowledge of chemistry and effective teaching methodologies.
- Constructed and administered tests and exams to measure student learning.
Post-doctoral Research Associate
Joint School Of Nanoscience And Nanoengineering
Greensboro, NC
12.2020 - 07.2021
- Toxicity evaluation of metal oxide nanomaterials with focus on mechanistic evaluation in lung epithelial cell lines using Neutral Red Uptake (NRU) and Lactose Dehydrogenase (LDH) assays
- Performed Enzyme-linked Immunosorbent assay (ELISA) to evaluate mechanism of toxicity.
Research Assistant
Joint School Of Nanoscience And Nanoengineering
01.2016 - 12.2020
- Toxicity Evaluation of Indium compounds (Indium oxide nanoparticles and InCl) on human lung Epithelial (A549) and endothelial (EA.hy926) cells -Characterization of nanoparticles using SEM, ICP-OES, FT-IR, DLS, zeta potential, and surface area
- In vitro cytotoxicity assessment by membrane integrity (LDH) and cell viability (Alamar blue) evaluation, and reactive oxygen species (ROS) production for dose-response assessment
- Cell culture was carried out in bio clean room following biosafety levels
- Cellular uptake, internalization and other changes from nanomaterial exposure and risk assessments were evaluated by ICP-OES, Confocal microscopy and CytoViva dark field microscopy
- Dissolution studies of InO nanoparticles in simulated lung fluids and performed DLS, ICP-OES and SEM on the post analysis centrifuge filtered fluid
- Conducted Epithelial (A549) and endothelial (EA.hy926) 2D cell culture studies to analyze the transport of Indium compounds through the air blood barrier
- TEER measurements to find the resistance of the barrier and ICP-OES to quantitatively analyze the Indium crossing the air blood barrier
- Statistical analysis of cytotoxicity data using ECHIP software and Minitab.
- Planned, modified and executed research techniques, procedures and tests.
- Attended seminars and symposiums to improve overall knowledge and understanding.
Synthetic Chemist
Syngenta Crop Protection
Greensboro, NC
10.2012 - 12.2013
- Worked as Synthetic Chemist in Process,
- Developed methods to synthesize crop protecting pesticides and herbicides resulting in effective cost reduction and product improvement
- Worked closely with the product integrity team in evaluating the risk to human health
- Established standard operating protocols for GC/MS. -Created technical reports and detailed record keeping in electronic notebook for the experiments conducted.
Research Assistant
North Carolina A&T State University
Greensboro, NC
01.2010 - 06.2012
- Synthetic structuring of Dopa mimetics for inhibition of the activity of the enzyme tyrosinase in the biosynthetic pathway of Melanin
- Synthesized amino acid derivatives of 3, 4 dimethoxy acetophenone, 6-methoxy tetralone and 5-methoxy indanone derivatives with the methoxy groups on the cyclic ring of ketones by using acid hydrolysis
- Synthesized hydroxyl amino acid derivatives of the ketones by decarboxylation and demethylation using acid hydrolysis, which is believed to have pharmacological activity
- Analyzed and interpreted all the products using HPLC, FTIR, NMR, GC/MS and UV-Vis
- Mentored undergraduates in synthesizing and analyzing organic compounds related to hydroxyl amino acid derivatives of ketones using analytical instruments GC/MS, NMR and HPLC.
Graduate Teaching Assistant
08.2009 - 05.2012
- Conducted undergraduate General Chemistry labs for 3 years involving Acid-Base titrations, determining pH using pH meter, viscosity using viscometer, specific gravity using hydrometer and density of liquids
- Tutored undergraduate level General chemistry course for 3 years.
Quality control Chemist
Heritage Pharmaceuticals Pvt. Ltd
06.2008 - 06.2009
- For 1 year in analyzing and interpreting the solid dosage forms using HPLC, GC/MS, UV-Vis and dissolution apparatus.
Education
PhD - Nanoengineering
North Carolina A&T State University
Master of Science - Chemistry
North Carolina A&T State University
Bachelor of Pharmacy - undefined
Jawaharlal Nehru Technological University
Skills
Cell culture: Mono and 2D cell culture
In vitro cytotoxicity assays: Neutral Red Uptake (NRU), Membrane integrity (LDH), Reactive oxygen species (ROS), Cell viability (Alamar blue)
Immunoassay: ELISA
Microscopy: Confocal microscopy, Scanning electron microscopy (SEM), Cytoviva Dark field microscopy
Analytical: HPLC, GC-MS, ICP-OES, FTIR, Raman, UV-Vis, FAAS, NMR,DLS, zeta potential analyzer and Column Chromatography
Computer Skills- Windows OS, Proficient in using MS Office, Matlab and Minitab
PUBLICATIONS
Mortensen, N. P., Pathmasiri, W., Snyder, R. W., Caffaro, M. M., Watson, S. L., Patel, P. R., Beeravalli, L., Prattipati, S., Aravamudhan, S., Sumner, S. J., & Fennell, T. R. (2022). Oral administration of TiO2 nanoparticles during early life impacts cardiac and neurobehavioral performance and metabolite profile in an age- and sex-related manner. Particle and fibre toxicology, 19(1), 3.
Mortensen, N.P.; Moreno
Mortensen, N.P.; MorenoCaffaro, M.; Aravamudhan, S.;Beeravalli, L.; Prattipati, S.; Snyder, R.W.; Watson, S.L.; Patel, P.R.; Weber,F.X.; Montgomery, S.A.; et al. Simulated Gastric Digestion and InVivo Intestinal Uptake of Orally Administered CuO Nanoparticles and TiO2E171 in Male and Female Rat Pups. Nanomaterials 2021,11, 1487.
Manuscripts in Preparation
Prattipati, S., Aravamudhan, S., Toxicity and uptake assessment of Indium materials (InO and InCl from InP wafer etching) of interest and in current use in the semiconductor industry.
Prattipati, S., Aravamudhan, S., Coculture and monoculture studies to evaluate translocation of inhaled InO NPs and InCl across the air blood barrier.
Prattipati, S., Aravamudhan, S., Dark Field Hyperspectral Imaging as a tool to analyze the intracellular localization of InO and InCl in cells.
Timeline
Adjunct Chemistry Instructor
North Carolina Agricultural And Technical State University
08.2021 - Current
Post-doctoral Research Associate
Joint School Of Nanoscience And Nanoengineering
12.2020 - 07.2021
Research Assistant
Joint School Of Nanoscience And Nanoengineering
01.2016 - 12.2020
Synthetic Chemist
Syngenta Crop Protection
10.2012 - 12.2013
Research Assistant
North Carolina A&T State University
01.2010 - 06.2012
Graduate Teaching Assistant
08.2009 - 05.2012
Quality control Chemist
Heritage Pharmaceuticals Pvt. Ltd
06.2008 - 06.2009
PhD - Nanoengineering
North Carolina A&T State University
Master of Science - Chemistry
North Carolina A&T State University
Bachelor of Pharmacy - undefined
Jawaharlal Nehru Technological University