Sivasangari Balakrishnan
Medford, Tiruchirappalli,Massachusetts, Tamil Nadu
Overview
14
14
years of post-secondary education
Work History
Associate professor
Dept of Biochemistry
Research Assistant Professor
Dr,Prabhakaran
Associate Director
University of Illinois
Chicago
- 60612 E-mail: praba.biopioneer@gmail.com, Research Resources Center Bioanalytics, Biophysics, and Cytomics Division Director, Flow Cytometry Core Shared Resources Core Leader, UI Cancer Center bganesh@uic.edu tel: 312-3550742, 312-996-2775
Education
Post-doctoral Research Associate - undefined
University of Illinois
01.2020 - 05.2022
Ph.D - Biochemistry
Bharathidasan University
01.2010 - 01.2016
M.Phil - Biochemistry
SASTRA University
01.2008 - 01.2009
M.Sc. - Biochemistry
SASTRA University
01.2006 - 01.2008
B. Sc. - Biochemistry
Mother Teresa University
01.2003 - 01.2006
Accomplishments
- University of Illinois Postdoctoral Research Associate- 2020-2022
- TCR independent Treg expansion to ameliorates autoimmune diseases Type 1 diabetes mellitus, Systemic lupus erythematosus
- Immunophenotyping to study dose dependent effect of OX40-Jagged1 on thymic Treg expansion
- Characterize the clones to express OX40 receptor in human peripheral blood to expand Treg to achieve therapeutic relevance
- Studied TFH dependent b-cell mediated immune responses in the pathogenesis of lupus nephritis (LN)
- Explore the role of OX40 L -Jagged 1 in amelioration of LN
- Junior Research Fellow- Department of Science & Technology 2011-2013
- Senior Research Fellow- Department of Science & Technology 2013-2014
- Worked in independent Department of Science & Technology sponsored project “Role of Class II histone deacetylases in the regulation of insulin gene transcription
- 2011-2014
- Explored the role of class II HDACs 4,5,7,9 expression at different concentration of glucose
- Studied the correlation of functional inactivation of class 2 HDACs in insulin gene expression “Effect of Acute inflammation on histone H3 modifications in Rat testis and its implications in Testicular Steroidogenesis”
- Characterized the molecular mechanisms of the inhibitory role of TNF-α on testicular steroidogenesis and gonadal functions
- TNF-α suppressed histone H3 acetylation as well as methylation, and concomitantly, increased the histone deacetylases activity in Leydig cells
- DAX1 as the key mediator of the TNF-α effect and demonstrated that knockdown of DAX1 restored normal steroidogenesis
- Summary of Ph.D
- Thesis Study on glucose-dependent expression of PAX6 and HDAC7 in the regulation of pancreatic β- cell function
- The paired homeodomain-containing transcription factor, paired box6 (PAX6), has shown to play pivotal roles in beta-cell function, including insulin biosynthesis and secretion
- However, glucose regulation of PAX6 and its role in pancreatic beta-cell function is not well understood
- Studies on the pathways of intracellular signal transduction that regulate Pax6 expression demonstrated that glucose mediated activation of PKC and JNK/p38 MAPK pathway down-regulates Pax6 expression in pancreatic beta-cells
- Suppression of Pax6 in high glucose, in turn, decreases the expression of PTEN while increasing cyclins A1/D1
- This may be one of the possible mechanisms subserving the high glucose-induced cell viability in beta-cells
- High glucose significantly increased histone H3 acetylation and concurrently decreased total HDAC activity
- Further, it was found that HDAC7 was significantly down-regulated in high glucose treated pancreatic beta-cells
- Experiments from siRNA mediated knockdown suggest that PAX6 regulates HDAC7 expression in response to glucose concentrations
- Furthermore, glucose by itself modulates the subcellular localization of HDAC7 through PKD1 mediated phosphorylation
- The chemical inhibition of HDAC7 significantly increased insulin mRNA levels at low glucose concentrations, suggesting its vital role in regulation of insulin gene transcription
- M Phil, Research work
- Clinical correlation among Non-alcoholic fatty liver disease, Type-2 Diabetes mellitus, Hepatocellular carcinoma 1)Haematological parameters such as WBC count, platelet count and Hb% were studied
- 2) Fasting blood glucose, post prandial blood glucose serum protein, Albumin: Globulin ratio and bilirubin were determined
- 3) Activities of liver marker enzymes such as serum transaminases (SGOT and SGPT) and alkaline phosphatase were studied 4) Alterations in the lipid profile including total cholesterol, triglycerides and
- HDL / LDL ratio were also studied
- Publications, Cell & Molecular Biology: Mammalian cell culture-Primary cell isolation(T,B, Macrophages, Dentritic cells ) and Maintaining immortalized cell lines, RNA isolation, cDNA preparation, RT-qPCR, Agarose Gel Electrophoresis, Mammalian cell transfection
- Cell fractionation, Polyacrylamide Gel Electrophoresis, Blotting techniques - Western blot, Indirect ELISA, Immunoprecipitation
- PBMC isolation, Isolation of CD3+, CD4+, Treg
- Immunofluorescence-Fixation (Cells & tissue), Embedding, Staining, Sectioning, Brdu staining, Confocal imaging
- Flow cytometry (upto 8 lasers)-Immunophenotyping, Apoptosis and necrosis, Cell cycle analysis
- Molecular (gene) Cloning, Colorimetric& Luminometric assays, Cell viability assays, Liquid chromatography
- Computational skills: Gene promoter analysis, Sequence alignment, Protein homology & Ab initio modeling and visualization tools, GraphPad Prism
- Workshops participated
- Hands-on training on High-Performance Liquid Chromatography and Mass spectrometry at Annamalai University, India
- National workshop on methods in InVitro Toxicology at Bharathidasan University
- Tiruchirappalli
- Workshops handled
- Handled a technical session in the National workshop on Basic Molecular Biological Techniques in QUEST-2013 at Bharathidasan University
- Tiruchirappalli, India.
Timeline
Post-doctoral Research Associate - undefined
University of Illinois
01.2020 - 05.2022
Ph.D - Biochemistry
Bharathidasan University
01.2010 - 01.2016
M.Phil - Biochemistry
SASTRA University
01.2008 - 01.2009
M.Sc. - Biochemistry
SASTRA University
01.2006 - 01.2008
B. Sc. - Biochemistry
Mother Teresa University
01.2003 - 01.2006
Associate professor
Dept of Biochemistry
Research Assistant Professor
Dr,Prabhakaran
Associate Director
University of Illinois
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