SM Ahasanul Kobir
Chaska,USA
Summary
Scientist with excellent productive research experience in multi-disciplinary areas of life sciences. Strong technical & problem-solving skills in virology (virus propagation, purification, quantification, detection, vaccine development, viral clearance assay, BSL2), microbiology(diagnostic & assay development), molecular biology(gene manipulation, cloning, gene expression, DNA microarray, NGS library preparation), cell biology(bacteria & mammalian cells), immunology, recombinant protein engineering, expression and purification, therapeutic drug development and in-vivo preclinical animal model study (pulmonary disease), infectious disease( tuberculosis and cholera), and industrial lab setting.
Demonstrated acumen in effective SOP development followed by ISO, ICH, FDA guidelines, independently experimental plan, assay development and validation, conceptual and innovative thinking, problem-solving, data analysis and results interpretation, report writing, and presented results to the higher management.
Experienced in team leading and project management (Certified Professional Project Manager), strong builder of collaborations in different aspects of industrial, basic, and translational research leading to long lasting 8 research projects, 2 pulmonary therapeutic drugs development, vaccine development, $1.2M Viral Clearance lab establishment and 12 scientific publications.
Overview
14
14
years of professional experience
Work History
Consultant
UAMS
05.2023 - Current
- Mentored lab students & stuffs for protein engineering and purification, assay development, innovative thinking, trouble shooting, root-cause analysis, result interpretation and analysis.
- Established a system wide interrogative signaling network using genomic, proteomic and biochemical techniques to identify a druggable biomarker to yield a new avenue to study and treat TNBC(triple-negative breast cancer) with precision medicine therapies.
Virologist, Lead Scientist
Bio-Techne corporation
03.2022 - 03.2023
- Established viral clearance lab (1.2 M USD project), leading team & bi-weekly meetings, managed multiple projects, independently designed, executed, and drew conclusions from scientific experiments, performed problem-solving, root cause analysis, results analysis & interpretation, report & SOPs development and assay validation, equipment (IQ, OQ, PQ) validations, as well as presented results to senior management.
- All developed SOPs were followed by ICH/FDA/ISO 13485 guidelines and company’s QA policy.
- Developed experimental design for virus detection & quantification, mammalian cell bank and virus bank (BSL 2 lab), scaling down of GMP antibody/protein production process & viral clearance evaluation during GMP protein/antibody production process.
- Techniques: Molecular biology (DNA/RNA extraction, primer/probe design, cDNA synthesis, qPCR and one step/two steps qRT-PCR), mammalian cell bank (CHO-S, HEK293, Vero, A9, PG-4, Mv1Lu), BSL2 viruses (xMulV, VSV, MVM, PRV) propagation, concentration (TFF, Amicon concentrator, ultracentrifugation) high titer virus stock, virus quantification (TCID50 assay, plaque assay, qPCR), viral clearance analysis (cytotoxicity, interference, qPCR, AKTA pure)
- Collaboration with GMP protein/ antibody development and gene therapy department units.
Technical Consultant
ICDDR,B
08.2018 - 12.2021
- Designed multi-epitope based in silico vaccine against West Nile virus and multi drug resistant strains of Mycobacterium tuberculosis by combining immunoinformatic and reverse vaccinology approaches
- Established molecular detection method for Mycobacterium Tuberculosis laboratory setting
- Mentored lab staff to develop experimental protocols, discuss scientific issues and solve experimental problems in molecular biology, cell biology, and immunology.
Postdoctoral Associate
UPMC Hillman Cancer Center
12.2016 - 07.2018
- Developed live cell gene expression analysis model using CRISPR/Cas9 to tag multi color fluorescence proteins into different loci in same chromosome to detect live cell gene expression after drug treatments
- Lentivirus vector construction, packaging, purification, concentration & infection (BSL-2)
- Stable cell line development using recombinant vector construction, transfection, lentiviral transduction, multicolor flow cytometry and selected biomarker (HUVEC, HEK293, U2os)
- Automated cell counter, ELISA, western blotting, IHC, PCR/RT-qPCR, DNA sequencing, multi-color flow cytometry, fluorescence anisotropy, TIRF, FRET, and confocal fluorescence microscopy techniques were used in the project.
Postdoctoral Associate
UPMC Heart and Vascular Institute
06.2015 - 06.2016
- Therapeutic drug development for pulmonary hypertension (PAH), i) mTOR kinase inhibitor 'PP242' ii) integrin-linked kinase1 inhibitor 'Cpd22' using in vivo preclinical animal study
- Independently planned, prepared, and managed in vivo pre-clinical trail from protocol development, data analysis, manuscript writing to publication
- Executed cell line development using gene cloning (Indel, SNP), plasmid/siRNA/shRNA transfection, clonal selection markers, sequencing, fluorescence imaging, automated cell counter
- Recombinant protein construction , expression, purification, optimize critical upstream and downstream protein purification parameters
- Protein functional determination using immunoblotting, IHC, IF, ELISA, luciferase assay, RT-qPCR
- Assay development and validation (proliferation /apoptosis/motility), NGS library preparation, generating CRISPR mutant mice and genotyping,
- Supervised lab students and stuffs.
Research Associate
The Scripps Research Institute
09.2014 - 05.2015
- Investigated the mechanism of bacterial cell wall homeostasis and its effect on innate immune system.
- Techniques: Gene cloning (insertion/deletion, SNP), fluorescence localization study, gene expression analysis (q-PCR, beta-gal assay), recombinant protein construction (HIS/GST-tag), expression and purification (affinity chromatography, AKTA pure), DNA sequencing, and antimicrobial drug sensitivity test
- Optimize critical upstream and downstream protein purification parameters
- Protein functional analysis (western blotting, protein-DNA binding affinity, co-Ip, radioactive protein phosphorylation assay)
Postdoctoral Fellow
Autonomous University of Madrid
01.2014 - 08.2014
- Investigated the molecular mechanism of RecA (DNA recombinase) phosphorylation in DNA damage and repair process in bacterial cells and/or in spore formation.
- Techniques: Gene cloning (insertion/deletion, SNP), gene expression analysis, recombinant protein fermentation (bench top bioreactor 10 L) & purification (IEC, HA column, HPLC), pDNA purification, radioactive protein phosphorylation assay etc
- Mentored lab co-workers, PhD and master students.
Graduate Research Student
INRA / CNRS
11.2009 - 06.2013
- Investigated the molecular mechanisms of bacterial genetics and signal transduction, focusing on the regulatory phenomena based on Ser/Thr protein phosphorylation and their role in cellular process, using metabolic engineering, functional genomics, transcriptomics & proteomics studies (B.subtilis, E.coli)
- Quantitative phosphoproteomics analysis of bacterial Ser/Thr kinases and phosphatases
- All projects are based on protein engineering, expression, purification, and these proteins qualitative and quantitative functional analysis.
- Molecular biology- Cloning (Indel, SNP), PCR/qPCR, gene regulation, gene expression analysis (qPCR b-gal assay, HTP live cell array), DNA/RNA purification, mRNA extraction and transcriptomic analysis, metabolic engineering, DNA sequencing, genomics, fluorescence microscopy etc
- Protein engineering & purification -Constructed protein expression vectors, proteins expression, purification (IEX/SEC/affinity chromatography, AKTA pure), proteomics, and protein characterization..
- Assay development (competence, sporulation, biofilm, motility, enzyme production, antibiotic sensitivity) and supervised master’s students, and lab co-workers.
Education
Certified Professional Project Manager -
University of ST. Thomas
Minneapolis01.2023
Ph.D. - Molecular Genetics
Université Paris-Sud
Paris, France01.2013
M.Sc - Food Technology And Processing
AgroParisTech
Paris, France01.2009
M.Sc - Molecular Genetics And Physiology
Linkoping University
Sweden01.2008
M.Sc - Food And Nutrition
University of Dhaka
Bangladesh01.2004
B.Sc - Biochemistry And Molecular Biology
Aligarh Muslim University
India01.2001
Skills
- Recombinant virus vector construction, and characterize DNA& RNA virus
- Virus propagation, concentration & purification (TFF, Amicon concentrator, ultracentrifuge), quantification (TCID50 & plaque assay, DNA/RNA extraction, cDNA synthesis, qPCR, RT-qPCR), cytotoxicity, high titer virus stock preparation (VSV, MuLV, lentivirus, MVM, PRV and more) BSL-2 lab
- Viral clearance assay to evaluate GMP protein/antibody production process
- Epitope based vaccine construction
- Immunoinformatics, bioinformatics
- Multicolor Flow cytometry, confocal fluorescence microscopy
- ELISA, western blotting, IHC, IF
- Molecular Biology (CRISPR, gene (insertion/deletion, SNP), vector construction, cloning, gene expression analysis (HTP live cell array, qPCR, beta gal)
- Functional genomics, transcriptomics, proteomics, NGS library preparation
- Many microbiological assay development and validation (SOPs & equipment (IQ, OQ, PQ)
- Bacterial & mammalian cell culture (Vero, PG-4, A9, Mv1lu, U2os, HEk293, CHO, HUVEC)
- In vivo preclinical animal model study
- Maintain GMP & GLP, ICH & FDA guidelines
- Industrial laboratory setup
- Project management
- Mentoring & critical thinking
- Experimental planning & execution
- Strong analytical & problem-solving
- Quantitative & qualitative analysis
- Report writing & SOP development
- Root-cause analysis
- Aseptic practice
- Computer/bioinformatics tools Microsoft Office, Photoshop, Image J, SAS, R, Python, FlowJo, SnapGene, Vector NTI, Neb cutter, BLAST, ClustalW2, ExPASy, VaxiJen v20, TMHMM, NetCTL, BLASTp, STRING etc
Personal Information
Citizenship: US Citizen
Timeline
Consultant
UAMS
05.2023 - Current
Virologist, Lead Scientist
Bio-Techne corporation
03.2022 - 03.2023
Technical Consultant
ICDDR,B
08.2018 - 12.2021
Postdoctoral Associate
UPMC Hillman Cancer Center
12.2016 - 07.2018
Postdoctoral Associate
UPMC Heart and Vascular Institute
06.2015 - 06.2016
Research Associate
The Scripps Research Institute
09.2014 - 05.2015
Postdoctoral Fellow
Autonomous University of Madrid
01.2014 - 08.2014
Graduate Research Student
INRA / CNRS
11.2009 - 06.2013
Certified Professional Project Manager -
University of ST. Thomas
Ph.D. - Molecular Genetics
Université Paris-Sud
M.Sc - Food Technology And Processing
AgroParisTech
M.Sc - Molecular Genetics And Physiology
Linkoping University
M.Sc - Food And Nutrition
University of Dhaka
B.Sc - Biochemistry And Molecular Biology
Aligarh Muslim University
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